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    POLYPEPTIDES TARGETING GLYCOSYLATED MUC2 PROTEINS, METHODS OF SYNTHESIS, THEIR NUCLEIC ACIDS AND USES THEREOF
    7.
    发明公开
    POLYPEPTIDES TARGETING GLYCOSYLATED MUC2 PROTEINS, METHODS OF SYNTHESIS, THEIR NUCLEIC ACIDS AND USES THEREOF 有权
    ON糖化蛋白MUC2多肽LOOKING，合成，及其核酸和方法及其用途

    公开(公告)号：EP2753639A1

    公开(公告)日：2014-07-16

    申请号：EP12755887.2

    申请日：2012-09-07

    申请人： Institut Pasteur

    发明人： MARTEYN, Benoit ,  COIC, Yves-Marie ,  BALEUX, Françoise ,  SANSONETTI, Philippe

    IPC分类号： C07K14/195 ,  C07K14/335 ,  A61K38/16 ,  C12N1/15 ,  C12N1/21 ,  C12N5/10 ,  G01N33/68 ,  G01N33/58

    CPC分类号： C07K14/335 ,  C07K7/08 ,  C07K14/195 ,  C07K14/315 ,  C07K2319/60 ,  C07K2319/61 ,  C12P21/02 ,  G01N33/6893

    摘要： The invention relates to polypeptides, defined through a consensus sequence, having a length from 10 to 80 amino-acid residues, and whose polypeptidic sequence comprises or consists of the consensus sequence P1 (X
    a )P3(X
    b )P5(X
    c )P6(X
    d )P7, presenting specific patterns. The polypeptides of the invention target glycosylated Muc2 proteins. The invention also relates to methods of synthesis of such polypeptides, to their nucleic acids and uses thereof. The polypeptidic sequence of the polypeptides of the invention can be part of the N-terminal sequence of a mucus-binding (MUB) domain, especially a mucus-binding (MUB) domain of several species. The invention also relates to chimeric molecule(s) comprising such polypeptides, which are labelled, and vectors, especially plasmids and population of cells or composition comprising polypeptides of the invention. Synthesis methods encompass biotechnological or chemical production. Polypeptides of the invention can be used in staining experiments, as a probe or marker for staining Muc2 protein(s) contained in mucus layer(s), to detect in vitro mucus production or mucus composition in human colon or monitoring any one of the following disease conditions: neoplasic disease(s), including mucinous carcinoma(s), gastric cancer(s) or colorectal cancer(s), especially colon cancer(s), cystic fibrosis, intestine inflammatory disease(s) such as inflammatory bowel disease (IBD) and ulcerative colitis. The invention also relates to a method for manufacturing a medicament. In a particular embodiment, use of polypeptides of the invention can be made for marking neutrophiles.

    摘要翻译： 本发明涉及通过共有序列定义的多肽，具有10至80个氨基酸残基，且其对多肽类序列包含或共有序列P1（XA）P3（X B）P5（X c）中的besteht长度 P6（X D）P7，呈现特定图案。 本发明目标的多肽糖基化的蛋白质MUC2。 因此，本发明涉及谋求多肽及其核酸及其用途的合成方法。 本发明的多肽的多肽性序列可以是粘液结合（MUB）结构域的N-末端序列的一部分，尤其是爱粘液结合（MUB）几个物种的结构域。 因此，本发明涉及嵌合分子（多个）被标记包括搜索多肽和载体，质粒特别喜欢和细胞或组合物包含本发明的多肽的群体。 合成方法包括生物技术和化工生产。 本发明的多肽可以在染色实验中使用，作为探针或标记物用于染色包含在粘液层（一个或多个）MUC2蛋白（S），以检测在体外粘液产生或在人结肠粘液组合物或监测以下中的任何一个 疾病病症：肿瘤性疾病（一个或多个），包括粘液癌（S），胃癌（S）或结肠直肠癌（S），特别是结肠癌（S），囊性纤维化，肠的炎性疾病（一个或多个）：如炎性肠疾病（ IBD）和溃疡性结肠炎。 因此本发明涉及一种用于制造药物的方法。 在特定实施例中，使用本发明的多肽可用于标记的中性粒细胞进行。