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    METHOD OF SELECTING BINDING MOLECULE
    2.
    发明公开
    METHOD OF SELECTING BINDING MOLECULE 审中-公开
    SELEKTIONSVERFAHRENFÜRBINDUNGSMOLEKÜLE

    公开(公告)号：EP1429143A1

    公开(公告)日：2004-06-16

    申请号：EP02760625.0

    申请日：2002-08-13

    申请人： Institute for Antibodies Co., Ltd.

    发明人： KUROSAWA, Yoshikazu ,  AKAHORI, Yasushi ,  HIRONO, Yukari ,  KAKITA, Mai ,  SUZUKI, Kazuhiro ,  OKUNO, Yoshinobu

    IPC分类号： G01N33/50 ,  A61K45/00 ,  A61K39/395 ,  G01N33/15

    CPC分类号： G01N33/54353 ,  C07K16/00 ,  C07K16/005 ,  C07K16/1018 ,  G01N33/53 ,  G01N33/5306 ,  G01N33/536

    摘要： A binding molecule that binds to a binding target substance is selected by introducing an affinity linker into the target binding substance and capturing the substance with a binding partner that binds to the affinity linker. For example, an antibody that binds to an influenza virus antigen can be easily concentrated by labeling a sugar chain present in the antigen with an affinity linker, such as biotin, and then recovering the antigen by binding them to streptavidin. The method for selecting binding molecules of the present invention is also useful in the screening of neutralizing substances.

    摘要翻译： 通过将亲和连接体引入目标结合物质并用与亲和连接子结合的结合配偶体捕获物质来选择与结合靶物质结合的结合分子。 例如，结合流感病毒抗原的抗体可以通过用亲和接头如生物素标记存在于抗原中的糖链来容易地浓缩，然后通过将其与链霉亲和素结合来回收抗原。 选择本发明的结合分子的方法也可用于筛选中和物质。

    METHOD OF CLASSIFYING ANTIGEN, METHOD OF IDENTIFYING ANTIGEN, METHOD OF OBTAINING ANTIBODY OR ANTIBODY SET, METHOD OF CONSTRUCTING ANTIBODY PANEL AND ANTIBODY OR ANTIBODY SET AND USE OF THE SAME
    4.
    发明公开
    METHOD OF CLASSIFYING ANTIGEN, METHOD OF IDENTIFYING ANTIGEN, METHOD OF OBTAINING ANTIBODY OR ANTIBODY SET, METHOD OF CONSTRUCTING ANTIBODY PANEL AND ANTIBODY OR ANTIBODY SET AND USE OF THE SAME 有权
    一种用于识别潜在的诊断或治疗性抗体的流式细胞术方法

    公开(公告)号：EP2078732A1

    公开(公告)日：2009-07-15

    申请号：EP07790510.7

    申请日：2007-07-09

    申请人： Institute for Antibodies Co., Ltd.

    发明人： SUGIOKA, Atsushi ,  SUGIURA, Mototaka ,  AKAHORI, Yasushi ,  HAYASHI, Nobuhiro ,  TAKASAKI, Akihiko ,  MORITA, Miwa ,  KUROSAWA, Gene ,  SUMITOMO, Mariko ,  TSUTSUMI, Susumu ,  OGAWA, Keiko ,  MATSUDA, Kazuki ,  MURAMATSU, Chiho ,  SATOU, Noriko ,  AZUMA, Masachika ,  UKAI, Yoshinori ,  SUZUKI, Kazuhiro ,  KUROSAWA, Yoshikazu ,  TANAKA, Miho ,  SHIRAISHI, Mamoru

    IPC分类号： C07K16/28 ,  C12N15/09 ,  C12Q1/02 ,  G01N33/574

    CPC分类号： C07K16/30 ,  C07K16/005 ,  C07K16/28 ,  C07K16/2803 ,  C07K16/2863 ,  C07K16/2896 ,  C07K16/303 ,  C07K16/32 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/73 ,  C07K2317/732 ,  C12N15/1138 ,  C12N2310/14 ,  G01N33/57492 ,  G01N33/6854

    摘要： It is intended to provide a method whereby a plural number of antibodies against cell surface antigens are quickly classified and to provide a method whereby antigens of the thus classified antibodies are quickly identified. Further, it is intended to provide a method of promoting the utilization of the useful data obtained by the above methods. Furthermore, it is intended to provide an antibody which is effective in treating or diagnosing cancer. Namely, a method of classifying antibodies which comprises: (1) the step of preparing a plural number of antibodies respectively recognizing cell surface antigens; (2) the step of bringing each of these antibodies into contact with a cell of the same species; (3) the step of analyzing each of the cells having been treated in the step (2) by flow cytometry and thus obtaining data indicating the reactivity of each antibody with its cell surface antigen; and (4) the step of comparing the thus obtained data and classifying the individual antibodies depending on the similarity. A method of identifying antigens which further comprises: (5) the step of selecting one to several antibodies from each antibody group formed in the step (4) and identifying antigens thereof; and (6) on the assumption that antigens of the antibodies belonging to a single antibody group are the same or highly related to one another, making relations between the antigens having been identified in the step (5) and the antibody groups to thereby identify the antigens. An antibody against HER1, an antibody against HER2, an antibody against CD46, an antibody against ITGA3, an antibody against ICAM1, an antibody against ALCAM, an antibody against CD147, an antibody against C1qR, an antibody against CD44, an antibody against CD73, an antibody against EpCAM and an antibody against HGFR, each obtained by using the above methods.

    摘要翻译： 它的目的是提供其中针对细胞表面抗原的抗体有多个被分类快速，是提供一种方法，由此将这样分类的抗体的抗原迅速识别的方法。 此外，它的目的是提供电视节目预告亭的方法通过上述方法获得的有用数据的利用。 进一步，它的目的是提供所有这是有效治疗或诊断癌症的抗体。 即，分类的抗体的方法，其包括：（1）制备抗体的分别认识细胞表面抗原的复数数的步骤; （2）使每个合成抗体与相同物种的细胞接触的步骤; （3）分析每个细胞的步骤中被处理，在步骤（2）通过流式细胞术和由此获得数据指示与它的细胞表面抗原，每种抗体的反应性; 和（4）比较由此获得的数据，并且根据该相似性的单个抗体分类的步骤。 识别抗原，另外还包括如下步骤：（5）选择一个，以从在步骤（4）以及它们的抗原识别形成的每个抗体组几种抗体的步骤; 和（6）在假设没有属于单个抗体组抗体的抗原相同或高度彼此相关，使得抗原之间的关系，在步骤已被鉴定（5）和抗体组，从而识别所述 抗原。 对HER1的抗体，以针对HER2抗体，对抗CD46抗体关于对ITGA3抗体抗ICAM-1抗体对ALCAM抗体针对CD147的抗体，对抗C1qR抗体对抗CD44抗体关于对CD73抗体， 针对EpCAM的并针对HGFR抗体抗体，每个通过使用上述方法获得的。

    METHOD OF CONSTRUCTING CAMEL ANTIBODY LIBRARY
    5.
    发明公开
    METHOD OF CONSTRUCTING CAMEL ANTIBODY LIBRARY 审中-公开
    VERFAHREN ZUM ERSTELLEN EINER KAMEL-ANTIKÖRPERBIBLIOTHEK

    公开(公告)号：EP1433793A1

    公开(公告)日：2004-06-30

    申请号：EP02763029.2

    申请日：2002-09-13

    申请人： Institute for Antibodies Co., Ltd.

    发明人： HONDA, Toshio ,  AKAHORI, Yasushi ,  KUROSAWA, Yoshikazu

    IPC分类号： C07K16/18 ,  C12N15/09

    CPC分类号： C07K16/40 ,  C07K16/00 ,  C07K16/005 ,  C07K2317/22 ,  C07K2317/52 ,  C07K2317/569

    摘要： The present invention provides camel antibody libraries that maintain in vivo diversity of camelid antibody variable region genes. The in vivo diversity of antibody variable region genes can be accomplished by, for example, mixing genes derived from a plurality of animals or modifying gene amplification conditions. Conventional methods yield only VHHs with limited repertoire diversity. However, the present invention provides libraries comprising genes encoding functional VHHs with sufficient repertoire size. According to the present invention, libraries that enable to freely obtain VHHs against arbitrary antigens are provided. VHHs have excellent solubility and stability, and show a reactivity that usually cannot be expected from tetrameric IgGs.

    摘要翻译： 本发明提供了保持骆驼抗体可变区基因的体内多样性的骆驼抗体文库。 抗体可变区基因的体内多样性可以通过例如混合来自多个动物的基因或修饰基因扩增条件来实现。 常规方法仅产生具有有限谱库多样性的VHH。 然而，本发明提供了包含编码具有足够谱库大小的功能性VHH的基因的文库。 根据本发明，提供能够自由获得针对任意抗原的VHH的文库。 VHH具有优异的溶解性和稳定性，并且显示通常不能从四聚体IgGs预期的反应性。