公开(公告)号：EP0822992A1

公开(公告)日：1998-02-11

申请号：EP96911209.0

申请日：1996-02-27

申请人： Intelligene Ltd.

发明人： ASHER, Nathan ,  TIKOCHINSKI, Yaron ,  KRUPP, Guido ,  GRINBERG, Jacob ,  FRIEDMANN, Adam

IPC分类号： C12N15 ,  C12N9 ,  C12Q1 ,  G01N33

CPC分类号： G01N33/581 ,  C12N15/113 ,  C12N2310/121 ,  C12N2310/321 ,  C12N2310/53 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6865 ,  C12Q2521/337 ,  C12Q2525/143 ,  C12Q2525/301 ,  C12Q2525/149 ,  C12N2310/3521

摘要： The present invention concerns a method for detecting the presence of a catalytically active ribozyme in a medium. The detection of the catalytically active ribozyme may be a goal by itself, or the ribozyme may serve as a reporter for the presence of other biomolecules in an assayed sample. The detection is carried out in a catalytic system wherein the presence of the active ribozyme serves to produce other active ribozyme in a positive-feedback amplificatory manner.