公开(公告)号：EP1732021A1

公开(公告)日：2006-12-13

申请号：EP05721340.7

申请日：2005-03-23

申请人： Bio-Think Tank Co., Ltd.

发明人： MORISHITA, Shinichi ,  YAMADA, Tomoyuki ,  NAITO, Yuki

IPC分类号： G06F19/00 ,  C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G06F17/30

CPC分类号： G06F19/20 ,  C12Q1/6869 ,  G06F19/22 ,  C12Q2525/149

摘要： It is intended to efficiently determine a base sequence specifically appearing in an expression gene. For this, providing that the expression gene consists of exons (301)...(306) and especially that exon (301) is united with exon (302) and exon (302) with exon (303), an aggregate of base sequences (401) (403) being a union of exon base sequences (301)...(305) and a boundary base sequence obtained by uniting together base sequences(404) and (405) and base sequences (406) and (407) respectively existing over boundaries between exon (301) and exon (302) and between exon (302) and exon (303) is formed, and the aggregate is searched. If a base sequence is one specifically appearing in the expression gene, the number of search results is 1 and otherwise, the number is plural.

摘要翻译： 旨在有效地确定特异性出现在表达基因中的碱基序列。 为此，提供表达基因由外显子（301）...（306）组成，特别是外显子（301）与外显子（302）和外显子（302）与外显子（303）结合，碱基序列 （401）（403）是通过将基本序列（404）和（405）以及碱基序列（406）和（407）组合在一起而获得的外显子碱基序列（301）...（305）和边界碱基序列 分别存在于外显子（301）和外显子（302）之间以及外显子（302）和外显子（303）之间的边界上，并且搜索聚集体。 如果碱基序列是表达基因中特定出现的碱基序列，则搜索结果数为1，否则为数。

公开(公告)号：EP0822992A4

公开(公告)日：2000-08-09

申请号：EP96911209

申请日：1996-02-27

申请人： INTELLIGENE LTD

发明人： ASHER NATHAN ,  TIKOCHINSKI YARON ,  KRUPP GUIDO ,  GRINBERG JACOB ,  FRIEDMANN ADAM

IPC分类号： C12N15/09 ,  C12N9/00 ,  C12N15/113 ,  C12Q1/68 ,  G01N33/58 ,  C12N15/00

CPC分类号： G01N33/581 ,  C12N15/113 ,  C12N2310/121 ,  C12N2310/321 ,  C12N2310/53 ,  C12Q1/6816 ,  C12Q1/682 ,  C12Q1/6865 ,  C12Q2521/337 ,  C12Q2525/143 ,  C12Q2525/301 ,  C12Q2525/149 ,  C12N2310/3521

摘要： The present invention concerns a method for detecting the presence of a catalytically active ribozyme in a medium. The detection of the catalytically active ribozyme may be a goal by itself, or the ribozyme may serve as a reporter for the presence of other biomolecules in an assayed sample. The detection is carried out in a catalytic system wherein the presence of the active ribozyme serves to produce other active ribozyme in a positive-feedback amplificatory manner.

公开(公告)号：EP2917349A2

公开(公告)日：2015-09-16

申请号：EP13792230.8

申请日：2013-11-06

申请人： President and Fellows of Harvard College ,  Trustees of Boston University

发明人： GREEN, Alexander, A. ,  YIN, Peng ,  COLLINS, James, J.

IPC分类号： C12N15/113 ,  C12N15/115 ,  C12Q1/68 ,  C12N15/67

CPC分类号： C12N15/11 ,  C12N15/113 ,  C12N15/115 ,  C12N15/67 ,  C12N2310/17 ,  C12N2310/531 ,  C12N2320/11 ,  C12N2320/12 ,  C12Q1/6816 ,  C12Q1/6897 ,  C12Q2525/143 ,  C12Q2525/149 ,  C12Q2525/301

摘要： The invention provides novel and versatile classes of riboregulators, including inter alia activating and repressing riboregulators, switches, and trigger and sink RNA, and methods of their use for detecting RNAs in a sample such as a well and in modulating protein synthesis and expression.

摘要翻译： 本发明提供新颖和多功能的类型的核糖调节剂，包括尤其是激活和抑制核糖体调节剂，开关和触发和汇集RNA，以及它们用于检测样品如孔中的RNA和调节蛋白质合成和表达的方法。

公开(公告)号：EP2241638B1

公开(公告)日：2013-11-27

申请号：EP10168634.3

申请日：2003-04-11

申请人： Esoterix Genetic Laboratories, LLC

发明人： Shuber, Anthony

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2523/125 ,  C12Q2549/119 ,  C12Q2525/149

公开(公告)号：EP1527199A4

公开(公告)日：2006-02-15

申请号：EP03746704

申请日：2003-04-11

申请人： EXACT SCIENCES CORP

发明人： SHUBER ANTHONY P

IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2523/125 ,  C12Q2549/119 ,  C12Q2525/149

摘要： The invention provides amplification-based methods for detecting hypermethylated or hypomethylated nucleic acid in heterogeneous biological samples, e.g., stool. A screening procedure based on the detection of hypermethylation, preferably at multiples genes, provides a means for detecting various diseases, e.g., colorectal cancer. By using chimeric primers that contain a 5' non-specific portion, the specificity and efficiency of the nucleic acid amplification is improved. Methods of the invention are especially useful in detection of rare events in a heterogeneous sample.

公开(公告)号：EP1474504A2

公开(公告)日：2004-11-10

申请号：EP03708879.6

申请日：2003-01-24

申请人： Pointilliste, Inc.

发明人： AULT-RICHE, Dana ,  KASSNER, Paul, D.

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： C40B30/04 ,  C12Q1/6837 ,  G01N33/54306 ,  G01N33/58 ,  G01N33/6803 ,  G01N2458/10 ,  C12Q2563/131 ,  C12Q2525/149 ,  C12Q2563/149

摘要： Provided are the use of collections of binding proteins, called capture agents, and their cognate binding partners, called tagged protein libraries, herein for profiling samples. Methods for generating the capture agents, tagged protein libraries and samples are also provided.

公开(公告)号：EP1451355A1

公开(公告)日：2004-09-01

申请号：EP02788472.5

申请日：2002-11-11

申请人： Compugen Ltd.

发明人： LEVANON, Erez ,  POLLOCK, Sarah ,  NEMZER, Sergey ,  SHOSHAN, Avi ,  KHOSRAVI, Rami ,  WALACH, Shira ,  LEVINE, Zurit ,  BERNSTEIN, Jeanne ,  DAHARI, Dvir ,  WASSERMAN, Alon ,  ROTMAN, Galit

IPC分类号： C12Q1/68

CPC分类号： G06F19/18 ,  C12Q1/6837 ,  G06F19/20 ,  G06F19/22 ,  C12Q2525/149

摘要： A method of identifying putative naturally occurring antisense transcripts is provided. The method is effected by (a) computationally aligning a first database including sense-oriented polynucleotide sequences with a second database including expressed polynucleotide sequences; and (b) identifying expressed polynucleotide sequences from the second database being capable of forming a duplex with at least one sense-oriented polynucleotide sequence of the first database, thereby identifying putative naturally occurring antisense transcripts.

公开(公告)号：EP1062367A4

公开(公告)日：2003-07-30

申请号：EP99914694

申请日：1999-02-22

申请人： JOHNSON & JOHNSON RES PTY LTD

发明人： TODD ALISON ,  FUERY CAROLINE J ,  CAIRNS MURRAY

IPC分类号： C12N15/09 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12Q1/6816 ,  C12Q1/6823 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6867 ,  C12Q2545/114 ,  C12Q2537/143 ,  C12Q2521/337 ,  C12Q2525/149 ,  C12Q2525/161 ,  C12Q2563/125 ,  C12Q2531/125 ,  C12Q2531/113

摘要： This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.

公开(公告)号：EP3216870A1

公开(公告)日：2017-09-13

申请号：EP17167258.7

申请日：2013-11-06

申请人： President and Fellows of Harvard College ,  Trustees of Boston University

发明人： Green, Alexander A. ,  Yin, Peng ,  Collins, James

IPC分类号： C12N15/113 ,  C12N15/11 ,  C12Q1/68 ,  C12N15/67

CPC分类号： C12N15/11 ,  C12N15/113 ,  C12N15/115 ,  C12N15/67 ,  C12N2310/17 ,  C12N2310/531 ,  C12N2320/11 ,  C12N2320/12 ,  C12Q1/6816 ,  C12Q1/6897 ,  C12Q2525/143 ,  C12Q2525/149 ,  C12Q2525/301

摘要： The invention provides novel and versatile classes of riboregulators, including inter alia activating and repressing riboregulators, switches, and trigger and sink RNA, and methods of their use for detecting RNAs in a sample such as a well and in modulating protein synthesis and expression.

摘要翻译： 本发明提供了新型和通用类型的核糖调节剂，尤其包括激活和抑制核糖调节剂，开关以及触发和汇聚RNA，以及它们用于检测样品例如孔中的RNA和调节蛋白质合成和表达的方法。

公开(公告)号：EP2241638A1

公开(公告)日：2010-10-20

申请号：EP10168634.3

申请日：2003-04-11

申请人： Genzyme Corporation

发明人： Shuber, Anthony

IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

CPC分类号： C12Q1/686 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6848 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/154 ,  C12Q2531/113 ,  C12Q2525/161 ,  C12Q2523/125 ,  C12Q2549/119 ,  C12Q2525/149

摘要： The invention provides amplification-based methods for detecting hypermethylated or hypomethylated nucleic acid in heterogeneous biological samples, e.g., stool. A screening procedure based on the detection of hypermethylation, preferably at multiples genes, provides a means for detecting various diseases, e.g., colorectal cancer. By using chimeric primers that contain a 5' non-specific portion, the specificity and efficiency of the nucleic acid amplification is improved. Methods of the invention are especially useful in detection of rare events in a heterogeneous sample.

摘要翻译： 本发明提供了用于检测异构生物样品例如粪便中的高甲基化或低甲基化核酸的基于扩增的方法。 基于超甲基化检测，优选多倍基因的筛选方法提供了检测各种疾病例如结肠直肠癌的手段。 通过使用含有5'非特异性部分的嵌合引物，提高了核酸扩增的特异性和效率。 本发明的方法在异质样品中罕见事件的检测中特别有用。