公开(公告)号：EP1198448A2

公开(公告)日：2002-04-24

申请号：EP00950379.8

申请日：2000-07-11

申请人： Ivax Corporation

发明人： JEKKEL, Antonia ,  AMBRUS, Gabor ,  ILKOY, Eva ,  HORVATH, Ildiko ,  KONYA, Attila ,  SZABO, Istvan, Mihaly ,  NAGY, Zsuzsanna ,  HORVATH, Gyula ,  MOZES, Julianna ,  BARTA, Istvan ,  SOMOGYI, Gyorgy ,  SALAT, Janos ,  BOROS, Sandor

IPC分类号： C07C69/74 ,  C07C61/12 ,  C07C67/02

CPC分类号： C12R1/29 ,  C12P7/42 ,  C12P17/06

摘要： The present invention relates to a new microbial process for the preparation of the compound of formula (I) from a compound of general formula (II) wherein R+ stands for an alkali metal or ammonium ion, by the submerged cultivation of the strain able to 6β-hydroxylate a compound of formula (II) in aerobic fermentation and by the separation and purification of the product of formula (I) formed in the course of the bioconversion, which comprises cultivating a strain of the genera Micromonospora able to 6β-hydroxylate a compound of the general formula (II), wherein R+ is as defined above, on a nutrient medium containing assimilable carbon and nitrogen sources and mineral salts at 25-32 °C, thereafter feeding the substrate to be transformed into the developed culture, then fermenting the substrate until the end of bioconversion, then separating the compound of formula (I) from the culture broth and, if desired, purifying the same.