公开(公告)号：EP2741072A4

公开(公告)日：2015-07-08

申请号：EP12819852

申请日：2012-07-31

Applicant: JAPAN SCIENCE & TECH AGENCY

Inventor： SHIGEKAWA HIDEMI ,  TAKEUCHI OSAMU

IPC: G01N21/17 ,  B82Y35/00 ,  G01N21/55 ,  G01N21/63 ,  G01Q60/12 ,  H01J37/244 ,  H01J37/26

CPC classification number: G01N21/55 ,  B82Y35/00 ,  G01N21/1717 ,  G01N21/636 ,  G01N2021/1719 ,  G01N2021/1725 ,  G01N2021/1789 ,  G01N2021/1791 ,  G01N2201/0697 ,  G01Q60/12 ,  H01J37/244 ,  H01J37/26 ,  H01J2237/24507 ,  H01J2237/24564 ,  H01J2237/2818

Abstract: A pump probe measuring device (1) comprises: an ultrashort optical pulse laser generator (2) for generating a first ultrashort optical pulse train which is a pump light (3a), second and third ultrashort optical pulse trains (3b), (3c) which are probe lights; an optical shutter unit (6) to which the second and the third ultrashort pulse trains (3b), (3c) are introduced; and a detecting unit (20) including an irradiation optical system (8) for directing the pump light (3a), the first probe light (3b) and the second probe light (3c) to a sample (7), a sensor (11) for detecting a probe signal from the sample (7), and a phase-sensitive detecting means (12) connected to the sensor (11). An optical shutter control unit (10) periodically modulates the delay time of the first probe light (3b) and that of the second probe light (3c) with respect to the pump light (3a), and the modulated first and second probe lights (3a), (3b) illuminate the sample (7) alternately to detect the probe signals from the sample (7) by the phase-sensitive detecting means (12) in synchronization with the periodic modulation signal of the delay time.

公开(公告)号：EP1306005A4

公开(公告)日：2005-12-07

申请号：EP01953338

申请日：2001-07-31

Applicant: JAPAN SCIENCE & TECH AGENCY

Inventor： AKIRA SHIZUO ,  TAKEUCHI OSAMU

IPC: A01K67/027 ,  C07K7/08 ,  C07K14/705 ,  C12N5/10 ,  C12N15/09 ,  C12N15/85 ,  C12Q1/02 ,  C12Q1/68 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/569 ,  G01N33/68 ,  C12N5/00 ,  C12N15/00

CPC classification number: C12N15/8509 ,  A01K67/0276 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0337 ,  C07K14/705 ,  G01N33/5088

Abstract: Nonhuman animal models non-responsive to a myoplasma-origin lipoprotein/lipopeptide; and a method of screening an inhibitor or a promoter for the response to a mycoplasma-origin lipoprotein by using these animal models. A nonhuman animal model (for example, TLR6 knockout mouse) having the deletion of the gene function encoding a protein specifically recognizing a mycoplasma-origin lipoprotein such as TLR6 on its chromosome is constructed. By using this nonhuman animal model non-responsive to the mycoplasma-origin lipoprotein or immunocytes (macrophage, etc.) originating in this nonhuman animal model, a test substance and the mycoplasma-origin lipoprotein, the response to the mycoplasma-origin lipoprotein in the above-described non-human animal model or immunocytes is assayed and evaluated, thereby screening an inhibitor or a promoter for the response thereto.

公开(公告)号：EP2090880A4

公开(公告)日：2012-09-12

申请号：EP07832710

申请日：2007-11-28

Applicant: JAPAN SCIENCE & TECH AGENCY

Inventor： SHIGEKAWA HIDEMI ,  TAKEUCHI OSAMU

IPC: G01Q30/02 ,  B82Y35/00 ,  G01B7/00 ,  G01N21/27 ,  G01N21/39 ,  G01N21/63 ,  G01Q60/10

CPC classification number: G01Q30/02 ,  G01N21/636

公开(公告)号：EP1460410A4

公开(公告)日：2005-04-06

申请号：EP02803925

申请日：2002-11-25

Applicant: JAPAN SCIENCE & TECH AGENCY

Inventor： SHIGEKAWA HIDEMI ,  TAKEUCHI OSAMU ,  YAMASHITA MIKIO ,  MORITA RYUJI

IPC: G01J1/42 ,  G01J3/28 ,  G01J3/433 ,  G01N21/64 ,  G01Q30/00 ,  G01Q30/02 ,  G01Q60/10 ,  G01Q60/24 ,  G01N13/12 ,  G01N13/16 ,  G12B21/22

CPC classification number: G01J3/433 ,  G01J3/2889 ,  G01J2001/4242 ,  G01N21/6408 ,  G01N21/6458 ,  G01N2021/6415 ,  G01Q30/02 ,  G01Q60/12 ,  Y10S977/85

Abstract: An apparatus for measuring a physical phenomenon caused by light excitation having an extreme resolution in both time and space domains, particularly a delay time modulation time-resolved probe scanning microscope. The apparatus comprises an ultra-short light laser pulse generator (2), a delay modulation circuit (6) for splitting an ultra-short light laser pulse (3) generated by the ultra-short light laser pulse generator (2) into two pulses and frequency-modulating (ω) the delay time td between the two ultra-short light laser pulses (4, 5), a scanning probe microscope (17), and a lock-in detector (8) for detecting lock-in of a probe signal of the microscope (17) with a modulation frequency (ω) of the delay time. The delay-time dependence of the probe signal (11) on the delay time is determined as a change of rate without being influenced by the fluctuation of the intensity of the ultra-short light laser pulse (3) and without thermal expansion and thermal contraction of the chip of the probe (19) caused by the application of the ultra-short light laser pulse (3). With a femtosecond-order time resolution and an angstrom-order space resolution, a light excitation physical phenomenon depending on the delay time between ultra-short light pulses can be measured.