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公开(公告)号：EP1062367B1
公开(公告)日：2008-11-12
申请号：EP99914694.7
申请日：1999-02-22
CPC分类号： C12Q1/686 , C12Q1/6816 , C12Q1/6823 , C12Q1/6844 , C12Q1/6853 , C12Q1/6867 , C12Q2545/114 , C12Q2537/143 , C12Q2521/337 , C12Q2525/149 , C12Q2525/161 , C12Q2563/125 , C12Q2531/125 , C12Q2531/113
摘要： This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.
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公开(公告)号：EP1546402A1
公开(公告)日：2005-06-29
申请号：EP03793475.9
申请日：2003-09-04
发明人： ARNDT, Gregory Martin , CAIRNS, Murray , TRAN, Nham , LAI, Angela
CPC分类号： C12N15/10 , C12N15/111 , C12N15/1132 , C12N15/1135 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： The present invention provides methods of producing dsDNA molecules that can be used to mediate RNA interference (RNAi). These methods include the production of hairpin DNAs including random sequences, and the use of convergent promoters to co-express sense and antisense RNAs. As such, the invention allows the production of random short hairpin RNA (shRNA) and amsll interfering RNA (siRNA) expression libraries for forward genetic screening.
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3.发明公开
公开(公告)号：EP1062367A1
公开(公告)日：2000-12-27
申请号：EP99914694.7
申请日：1999-02-22
CPC分类号： C12Q1/686 , C12Q1/6816 , C12Q1/6823 , C12Q1/6844 , C12Q1/6853 , C12Q1/6867 , C12Q2545/114 , C12Q2537/143 , C12Q2521/337 , C12Q2525/149 , C12Q2525/161 , C12Q2563/125 , C12Q2531/125 , C12Q2531/113
摘要： This application provides methods of detecting and quantitatively determining a target nucleic acid sequence in a sample, which comprise contacting the sample with a primer and a zymogene which encodes, but which itself is the anti-sense sequence of, a catalytic nucleic acid sequence, so that when the target is present, a single amplified nucleic acid molecule is produced which comprises the sequences of both the target and catalytic molecules. This invention further provides a method of simultaneously detecting the presence of a plurality of target nucleic acid sequences in a sample. Finally, this invention provides molecules and kits for practicing the instant methods.
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