公开(公告)号：EP3162809A4

公开(公告)日：2018-01-24

申请号：EP15812576

申请日：2015-06-25

申请人： JSR CORP ,  JSR LIFE SCIENCES CORP

发明人： MOMIYAMA MASAKI ,  TSUDA SACHIKO ,  NORINOBU TOMOYA

IPC分类号： C07K1/22 ,  B01D15/08 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28 ,  B01J20/32 ,  C07K16/00 ,  G01N30/26 ,  G01N30/88

CPC分类号： C07K1/22 ,  B01D15/08 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  C07K14/195 ,  C07K16/00 ,  G01N30/26 ,  G01N30/88

摘要： Provided is an affinity chromatography carrier that suppresses nonspecific adsorption of impurities, suppresses a decrease in dynamic binding capacity due to long-term storage, and has high storage stability. The affinity chromatography carrier includes a solid support including a copolymer including (M-1) more than 20 parts by mass and 99.5 parts by mass or less of a structural unit derived from an epoxy group-containing monovinyl monomer with respect to and (M-2) 0.5 to 80 parts by mass of a structural unit derived from a polyvinyl monomer with respect to all structural units; ring-opened epoxy groups obtained by subjecting the epoxy groups to the ring-opening ; and a ligand coupled to the solid support, and is such that after a certain column vessel is packed with the affinity chromatography carrier and then purged with a 2 M NaCl-containing 20 mM sodium phosphate buffer with a pH of 7.5, allowing the column to stand at 40°C for 7 days increases pH in the column by at most 2.

公开(公告)号：EP3162809A1

公开(公告)日：2017-05-03

申请号：EP15812576

申请日：2015-06-25

申请人： JSR CORP ,  JSR LIFE SCIENCES CORP

发明人： MOMIYAMA MASAKI ,  TSUDA SACHIKO ,  NORINOBU TOMOYA

IPC分类号： C07K1/22 ,  B01D15/08 ,  B01J20/24 ,  C07K16/00 ,  G01N30/26 ,  G01N30/88

CPC分类号： C07K1/22 ,  B01D15/08 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  C07K14/195 ,  C07K16/00 ,  G01N30/26 ,  G01N30/88

摘要： Provided is an affinity chromatography carrier that suppresses nonspecific adsorption of impurities, suppresses a decrease in dynamic binding capacity due to long-term storage, and has high storage stability. The affinity chromatography carrier includes a solid support including a copolymer including (M-1) more than 20 parts by mass and 99.5 parts by mass or less of a structural unit derived from an epoxy group-containing monovinyl monomer with respect to and (M-2) 0.5 to 80 parts by mass of a structural unit derived from a polyvinyl monomer with respect to all structural units; ring-opened epoxy groups obtained by subjecting the epoxy groups to the ring-opening ; and a ligand coupled to the solid support, and is such that after a certain column vessel is packed with the affinity chromatography carrier and then purged with a 2 M NaCl-containing 20 mM sodium phosphate buffer with a pH of 7.5, allowing the column to stand at 40°C for 7 days increases pH in the column by at most 2.

摘要翻译： 本发明提供一种抑制杂质的非特异性吸附，抑制因长期保存而引起的动态结合能力降低，且保存稳定性高的亲和性色谱载体。 亲和色谱载体包含固体载体，所述固体载体包含相对于（M-1）含有大于20质量份和小于等于99.5质量份的来自含环氧基的单乙烯基单体的结构单元的（M- 2）相对于全部结构单元为0.5〜80质量份源自聚乙烯基单体的结构单元; 通过对环氧基进行开环而得到的开环环氧基; 以及与固体支持物偶联的配体，并且使得在用亲和层析载体填充某个柱容器之后，然后用pH值为7.5的含有2M NaCl的20mM磷酸钠缓冲液清洗，使柱 在40℃静置7天使柱中的pH增加至多2。

公开(公告)号：EP3076170A4

公开(公告)日：2017-09-06

申请号：EP14865665

申请日：2014-11-27

申请人： JSR CORP ,  JSR LIFE SCIENCES CORP

发明人： MOMIYAMA MASAKI ,  TSUDA SACHIKO ,  OKANO YUSUKE ,  SHIOTANI TOMONORI ,  KAWADA YUKO ,  TSUDA RYOU ,  KAWAI HIROSHI

IPC分类号： B01J20/289 ,  B01D15/38 ,  B01J20/32 ,  G01N30/02 ,  G01N30/88

CPC分类号： B01J20/289 ,  B01D15/3804 ,  B01J20/321 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  G01N30/02

公开(公告)号：EP2562178A4

公开(公告)日：2013-08-14

申请号：EP11765573

申请日：2011-03-29

申请人： JSR CORP

发明人： TAMORI KOUJI ,  ABE TAKAYOSHI ,  OKANO YUSUKE ,  MOMIYAMA MASAKI ,  KAWAI HIROSHI ,  HYUGAJI SATOSHI ,  WANG YONG

IPC分类号： C07K1/22 ,  B01D15/38 ,  B01J20/286 ,  C07K1/18 ,  C07K16/00 ,  C08F8/00 ,  C08F20/20 ,  C08F20/32 ,  C08F222/10

CPC分类号： C07K1/18 ,  B01D15/3804 ,  B01D15/3809 ,  B01J20/286 ,  C07K1/22 ,  C08F222/1006 ,  C08F220/20 ,  C08F2220/325 ,  C08F2220/281 ,  C08F220/58

公开(公告)号：EP2339339A4

公开(公告)日：2016-10-12

申请号：EP09816172

申请日：2009-09-24

申请人： JSR CORP

发明人： TAMORI KOUJI ,  FUKUTA TETSUO ,  MIYAJI MASAAKI ,  WANG YONG ,  ABE TAKAYOSHI ,  OKANO YUUSUKE ,  MOMIYAMA MASAKI ,  KAWAI TAKAHIRO

IPC分类号： G01N30/88 ,  B01J20/286 ,  C07K1/22 ,  C07K14/31

CPC分类号： C07K1/22 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274 ,  Y10T428/29

摘要： An affinity chromatography packing material includes porous mother particles that include a copolymer of a monomer mixture including a crosslinkable vinyl monomer and an epoxy group-containing vinyl monomer, a ligand being bound to the porous mother particles, and the porous mother particles including a ring-opening epoxy group produced by ring-opening of the epoxy group included in the porous mother particles.

公开(公告)号：EP2574631A4

公开(公告)日：2013-12-04

申请号：EP11759404

申请日：2011-03-23

申请人： JSR CORP

发明人： TAMORI KOUJI ,  FUKUTA TETSUO ,  MIYAJI MASAAKI ,  WANG YONG ,  ABE TAKAYOSHI ,  OKANO YUSUKE ,  MOMIYAMA MASAKI ,  KAWAI TAKAHIRO

IPC分类号： C07K17/08 ,  B01J20/32 ,  C07K1/22 ,  C07K19/00 ,  C12N15/09 ,  G01N30/88

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/286 ,  B01J20/288 ,  B01J20/3219 ,  B01J20/3274 ,  C07K2317/10

摘要： Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier. €ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒ€ƒR-R 2 €ƒ€ƒ€ƒ€ƒ€ƒ(1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R 2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R 2 .