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公开(公告)号：EP0690132A1
公开(公告)日：1996-01-03
申请号：EP94909286.0
申请日：1994-03-09
发明人： EDA, Yasuyuki , MAEDA, Hiroaki , MAKIZUMI, Keiichi , SHIOSAKI, Kouichi , OSATOMI, Kiyoshi , KIMACHI, Kazuhiko , HIGUCHI, Hirofumi , TOKIYOSHI, Sachio
CPC分类号： C07K16/1063 , A61K38/00 , C07K2317/24
摘要： The invention provides a clinically useful monoclonal antibody which can recognize the conserved region of the V3-PND region of a glycoprotein antigen (gp120) having a molecular weight of about 120,000 Da and contained in the coat of a human immunodefiency virus (HIV) and which can neutralize a wide variety of HIV variants, or a fragment of the above antibody, and chimera and humanized antibodies derived from the above antibody. It is possible to prepare a monoclonal antibody having the activity of neutralizing various HIV variants by using as the immunogen several types of peptides having a PND tip region containing a GPGR sequence having a high conservability in the PND region of the gp120 of an HIV. It is possible to obtain a chimera antibody or an altered antibody having an anti-HIV neutralization activity effective for clinical application by transplanting a fragment of a gene coding for the variable region of the above monoclonal antibody or the complementarity-determining region of that region to a human antibody gene.
摘要翻译： 本发明提供临床有用的单克隆抗体，其可以识别分子量为约120,000Da且包含在人免疫缺陷病毒（HIV）的外壳中的糖蛋白抗原（gp120）的V3-PND区域的保守区域，并且其中 可以中和各种HIV变体，或上述抗体的片段，以及衍生自上述抗体的嵌合体和人源化抗体。 可以制备具有中和各种HIV变异体的活性的单克隆抗体，该单克隆抗体具有在HIV的gp120的PND区域中具有包含具有高保守性的GPGR序列的PND末端区域的几种类型的肽作为免疫原。 可以通过将编码上述单克隆抗体的可变区的基因的片段或该区域的互补决定区域移植到具有对临床应用有效的抗HIV中和活性的嵌合体抗体或改变的抗体 人抗体基因。
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公开(公告)号：EP0678523B1
公开(公告)日：2004-09-15
申请号：EP93901547.5
申请日：1993-01-14
发明人： MAEDA, Hiroaki , KIMACHI, Kazuhiko , EDA, Yasuyuki , SHIOSAKI, Kouichi , OSATOMI, Kiyoshi Pana Haitsu 102 , TOKIYOSHI, Sachio
IPC分类号： C07K16/10 , A61K39/395 , C12P21/08 , C12N15/13
CPC分类号： C07K16/1063 , A61K38/00 , C07K2319/00
摘要： The invention relates to a gene fragment which codes for the variable regions of an antibody having an activity of neutralizing human immunodeficiency virus (HIV), a novel recombinant anti-HIV antibody expressed by using the above gene, and a process for preparing the same. A mouse-human chimeric antibody and a modified mouse-human antibody, having an activity of neutralizing HIV, are obtained by determining a specific nucleic acid sequence of a gene fragment which codes for the variable regions of the H and L chains of an antibody having an activity of neutralizing HIV and artificially fusing a DNA synthesized on the basis of the determined sequence with a gene which codes for human immunoglobulin. The novel anti-HIV antibody is useful for treating and preventing AIDS.
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公开(公告)号：EP0690132B1
公开(公告)日：2003-12-17
申请号：EP94909286.0
申请日：1994-03-09
发明人： EDA, Yasuyuki , MAEDA, Hiroaki , MAKIZUMI, Keiichi , SHIOSAKI, Kouichi , OSATOMI, Kiyoshi , KIMACHI, Kazuhiko , HIGUCHI, Hirofumi , TOKIYOSHI, Sachio
CPC分类号： C07K16/1063 , A61K38/00 , C07K2317/24
摘要： The invention provides a clinically useful monoclonal antibody which can recognize the conserved region of the V3-PND region of a glycoprotein antigen (gp120) having a molecular weight of about 120,000 Da and contained in the coat of a human immunodefiency virus (HIV) and which can neutralize a wide variety of HIV variants, or a fragment of the above antibody, and chimera and humanized antibodies derived from the above antibody. It is possible to prepare a monoclonal antibody having the activity of neutralizing various HIV variants by using as the immunogen several types of peptides having a PND tip region containing a GPGR sequence having a high conservability in the PND region of the gp120 of an HIV. It is possible to obtain a chimera antibody or an altered antibody having an anti-HIV neutralization activity effective for clinical application by transplanting a fragment of a gene coding for the variable region of the above monoclonal antibody or the complementarity-determining region of that region to a human antibody gene.
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4.发明公开
公开(公告)号：EP0678523A1
公开(公告)日：1995-10-25
申请号：EP93901547.5
申请日：1993-01-14
发明人： MAEDA, Hiroaki , KIMACHI, Kazuhiko , EDA, Yasuyuki , SHIOSAKI, Kouichi , OSATOMI, Kiyoshi Pana Haitsu 102 , TOKIYOSHI, Sachio
CPC分类号： C07K16/1063 , A61K38/00 , C07K2319/00
摘要： The invention relates to a gene fragment which codes for the variable regions of an antibody having an activity of neutralizing human immunodeficiency virus (HIV), a novel recombinant anti-HIV antibody expressed by using the above gene, and a process for preparing the same. A mouse-human chimeric antibody and a modified mouse-human antibody, having an activity of neutralizing HIV, are obtained by determining a specific nucleic acid sequence of a gene fragment which codes for the variable regions of the H and L chains of an antibody having an activity of neutralizing HIV and artificially fusing a DNA synthesized on the basis of the determined sequence with a gene which codes for human immunoglobulin. The novel anti-HIV antibody is useful for treating and preventing AIDS.
摘要翻译： 本发明涉及编码具有中和人免疫缺陷病毒（HIV）活性的抗体的可变区的基因片段，使用上述基因表达的新型重组抗HIV抗体及其制备方法。 具有中和HIV活性的小鼠 - 人嵌合抗体和修饰的小鼠 - 人抗体通过确定编码具有下列特征的抗体的H链和L链的可变区的基因片段的特定核酸序列来获得： 中和HIV并基于确定的序列合成的DNA与编码人免疫球蛋白的基因人工融合的活性。 新型抗HIV抗体可用于治疗和预防艾滋病。
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