公开(公告)号：EP2508599B1

公开(公告)日：2016-04-20

申请号：EP10834471.4

申请日：2010-11-11

Applicant: Japan Science and Technology Agency

Inventor： KOBAYASHI Kazuto

IPC: C12N15/00 ,  A61K35/76 ,  A61K38/00 ,  A61K48/00 ,  A61P25/16 ,  C07K14/145 ,  C07K14/16 ,  C12N5/10 ,  C12N7/02 ,  C12N15/09

CPC classification number: A61K48/0075 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/16045 ,  C12N2740/16052 ,  C12N2760/20122 ,  C12N2760/20222 ,  C12N2810/6081

公开(公告)号：EP2508599A1

公开(公告)日：2012-10-10

申请号：EP10834471.4

申请日：2010-11-11

Applicant: Japan Science and Technology Agency

Inventor： KOBAYASHI Kazuto

IPC: C12N15/00 ,  A61K35/76 ,  A61K38/00 ,  A61K48/00 ,  A61P25/16 ,  C07K14/145 ,  C07K14/16 ,  C12N5/10 ,  C12N7/02 ,  C12N15/09

CPC classification number: A61K48/0075 ,  C07K14/005 ,  C12N15/86 ,  C12N2740/16045 ,  C12N2740/16052 ,  C12N2760/20122 ,  C12N2760/20222 ,  C12N2810/6081

Abstract: Provided is a lentiviral vector system which sustains a high-frequency retrograde transportation ability in animal brain and has a higher titer. A kit for preparing a retrograde transport viral vector, which comprises: (1) a packaging plasmid containing the gag gene and pol gene of HIV-1; (2) a packaging plasmid containing an accessory gene of HIV-1; (3) a transfer plasmid containing a target gene; and (4) an envelope plasmid containing, as an envelope gene, a gene encoding a fused polypeptide comprising the extracellular domain of rabies virus glycoprotein (RV-G), the transmembrane domain of rabies virus glycoprotein (RV-G) or vesicular stomatitis virus glycoprotein (VSV-G) and the intracellular domain of vesicular stomatitis virus glycoprotein (VSV-G).

Abstract translation: 提供了一种在动物脑中维持高频逆行运输能力并具有较高滴度的慢病毒载体系统。 一种用于制备逆行转运病毒载体的试剂盒，其包括：（1）含有HIV-1的gag基因和pol基因的包装质粒; （2）含有HIV-1辅助基因的包装质粒; （3）含有靶基因的转移质粒; （4）包含作为包膜基因的包含狂犬病病毒糖蛋白（RV-G）细胞外结构域的融合多肽的基因，狂犬病病毒糖蛋白（RV-G）或水泡性口炎病毒的跨膜结构域的包膜质粒 糖蛋白（VSV-G）和水泡性口炎病毒糖蛋白（VSV-G）的细胞内结构域。

公开(公告)号：EP2644697B1

公开(公告)日：2018-12-26

申请号：EP11843579.1

申请日：2011-11-25

Applicant: Japan Science and Technology Agency

Inventor： KOBAYASHI Kazuto

IPC: C12N15/00 ,  A61K35/76 ,  A61K48/00 ,  A61P25/00 ,  A61P25/16 ,  C12N5/10 ,  C12N15/09 ,  C12N7/02 ,  C07K14/005 ,  C12N15/86

CPC classification number: C12N15/86 ,  A61K48/0058 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/03 ,  C12N2740/16045 ,  C12N2740/16071 ,  C12N2760/20122 ,  C12N2760/20222 ,  C12N2810/6081

Abstract: The purpose of the present invention is to provide a lentiviral vector system having a higher titer, while sustaining an excellent retrograde transport ability particularly in the brain. The present invention relates to a kit for preparing a retrograde transport viral vector comprising: (1) a packaging plasmid containing the gag gene and the pol gene of HIV-1; (2) a packaging plasmid containing an accessory gene of HIV-1; (3) a transfer plasmid containing an target gene (a transgene); and (4) an envelope plasmid containing, as an envelope gene, a gene encoding a fused polypeptide comprising a fused extracellular domain consisting of the N-terminal region of an extracellular domain of rabies virus glycoprotein (RV-G) and the C-terminal region of an extracellular domain of vesicular stomatitis virus glycoprotein (VSV-G), a transmembrane domain of RV-G orVSV-G, and an intracellular domain of VSV-G., and the like.

公开(公告)号：EP2644697A1

公开(公告)日：2013-10-02

申请号：EP11843579.1

申请日：2011-11-25

Applicant: Japan Science and Technology Agency

Inventor： KOBAYASHI Kazuto

IPC: C12N15/00 ,  A61K35/76 ,  A61K48/00 ,  A61P25/00 ,  A61P25/16 ,  C12N5/10 ,  C12N15/09 ,  C12N7/02

CPC classification number: C12N15/86 ,  A61K48/0058 ,  C07K14/005 ,  C07K2319/00 ,  C07K2319/03 ,  C12N2740/16045 ,  C12N2740/16071 ,  C12N2760/20122 ,  C12N2760/20222 ,  C12N2810/6081

Abstract: The purpose of the present invention is to provide a lentiviral vector system having a higher titer, while sustaining an excellent retrograde transport ability particularly in the brain.
The present invention relates to a kit for preparing a retrograde transport viral vector comprising:
(1) a packaging plasmid containing the gag gene and the pol gene of HIV-1;
(2) a packaging plasmid containing an accessory gene of HIV-1;
(3) a transfer plasmid containing an target gene (a transgene); and
(4) an envelope plasmid containing, as an envelope gene, a gene encoding a fused polypeptide comprising a fused extracellular domain consisting of the N-terminal region of an extracellular domain of rabies virus glycoprotein (RV-G) and the C-terminal region of an extracellular domain of vesicular stomatitis virus glycoprotein (VSV-G), a transmembrane domain of RV-G orVSV-G, and an intracellular domain of VSV-G., and the like.