公开(公告)号：EP1909104B1

公开(公告)日：2010-07-21

申请号：EP06746921.3

申请日：2006-05-26

申请人： Japan Science and Technology Agency ,  National University Corporation Kagoshima University ,  Suda, Yasuo

发明人： SUDA, Yasuo ,  NISHIMURA, Tomoaki ,  KISHIMOTO, Yuko ,  NAKAGAWA, Hiromi

IPC分类号： G01N33/543 ,  G01N33/553 ,  G01N33/58 ,  C07K1/22

CPC分类号： C07K17/14 ,  B82Y5/00 ,  C07K1/22 ,  G01N33/54346 ,  G01N33/553 ,  G01N33/587 ,  G01N2400/00

摘要： It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling.

公开(公告)号：EP1909104A1

公开(公告)日：2008-04-09

申请号：EP06746921.3

申请日：2006-05-26

申请人： Japan Science and Technology Agency ,  National University Corporation Kagoshima University ,  Suda, Yasuo

发明人： NISHIMURA, Tomoaki ,  KISHIMOTO, Yuko ,  NAKAGAWA, Hiromi ,  SUDA, Yasuo

IPC分类号： G01N33/543 ,  C07K1/22 ,  G01N33/553

CPC分类号： C07K17/14 ,  B82Y5/00 ,  C07K1/22 ,  G01N33/54346 ,  G01N33/553 ,  G01N33/587 ,  G01N2400/00

摘要： It is intended to provide a stable novel sugar-immobilized metal nanoparticle capable of easily immobilizing a sugar chain, a method for measuring sugar-protein interaction easily and at a low cost using the same without labeling, and a method for simply recovering a protein from a sugar-protein interactant. A maltose-immobilized gold nanoparticle was obtained by binding a ligand complex, in which maltose and a linker compound had been bound to each other, to a gold nanoparticle. By adding this maltose-immobilized gold nanoparticle to a dilution series of concanavalin A, a sugar-protein interactant of maltose and ConA was formed, and red-purple color derived from a colloidal solution of maltose-immobilized gold nanoparticle disappeared. That is, sugar-protein interaction could be confirmed by visual observation without labeling.

摘要翻译： 它的目的是提供一个稳定的新颖糖固定化的金属纳米颗粒能够容易地固定的糖链中，用于测量糖 - 蛋白质相互作用容易且使用相同的无标记的低成本的方法，以及用于简单地从回收的蛋白质的方法 糖蛋白相互作用物。 麦芽糖固定金纳米粒子通过什么结合的配体复合物，其中麦芽糖和左复合已被绑定到海誓山盟，到金纳米粒子获得。 通过添加该麦芽糖固定化的金纳米颗粒的稀释系列伴刀豆球蛋白A的，麦芽糖和刀豆的糖 - 蛋白相互作用物从麦芽糖固定化金纳米颗粒的胶体溶液衍生什么形成，并且红紫色消失。 即，糖 - 蛋白质相互作用可通过无标记视觉观察来确认。