公开(公告)号：EP1645621A1

公开(公告)日：2006-04-12

申请号：EP04730647.7

申请日：2004-04-30

申请人： Japan Science and Technology Corporation

发明人： YASUDA, Kenji ,  TAKAHASHI, Kazunori

IPC分类号： C12M1/00 ,  C12N15/09 ,  C12N5/06

CPC分类号： C12M47/04 ,  B01L3/502761 ,  B01L2300/0681 ,  B01L2400/0421 ,  B01L2400/043 ,  B01L2400/0439 ,  B01L2400/086

摘要： A cell separation apparatus which do not damage a cell sample, prevent the exhaustion of an electrode to which an electric voltage is applied in the separation of a cell, and does not cause the clogging of channels over a long period of term for cell separation, is provided.
The cell separation apparatus of the present invention comprises a means for moving a cell within a cell separating space by applying an external force to cells in the cell separating space from outside, and channels for separating and discharging the cell, which thus does not damage a cell sample and prevents the exhaustion of an electrode due to its electrolysis. As a means for applying an external force, a gel electrode containing electrolyte is preferred to apply an electric voltage to a cell separating space.
The cell separation apparatus may further comprise a filter disposed at upstream of a channel through which a sample to be introduced into a cell separating space is passed, whereby impurities can be captured so that the clogging of channels is prevented.

摘要翻译： 不破坏细胞样品的细胞分离装置，在细胞分离中防止施加电压的电极的耗尽，并且不会长期使细胞堵塞而使细胞分离， 被提供。 本发明的细胞分离装置包括：通过向细胞分离空间内的细胞施加外力而在细胞分离空间内移动细胞的装置，以及不会破坏细胞分离空间的通道 细胞样品并防止由于电解而导致的电极耗尽。 作为施加外力的手段，优选含有电解质的凝胶电极向电池分离空间施加电压。 细胞分离装置还可以包括设置在通道上游的过滤器，通过该过滤器将待引入细胞分离空间的样品通过，从而可以捕获杂质，从而防止通道堵塞。

公开(公告)号：EP1609851A1

公开(公告)日：2005-12-28

申请号：EP04712723.8

申请日：2004-02-19

申请人： Japan Science and Technology Corporation

发明人： HATTORI, Akihiro ,  YASUDA, Kenji

IPC分类号： C12M3/00 ,  C12M1/38 ,  C12N5/06 ,  G01N33/48

CPC分类号： C12M23/16 ,  B01J2219/00317 ,  B01J2219/00441 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00743 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/12 ,  B01L2300/163

摘要： There is provided a processing apparatus and a processing method for micro-chamber for cell culture having a desired culturing space.
The micro-chamber processing apparatus for cell culture of the present invention comprises a micro-chamber comprising no more than one absorption layer and at least one gel layer, in this order, laminated on a transparent base plate having no conspicuous absorbency in visible and infrared regions, and at least one light source, said absorption layer having absorbency in visible and infrared regions, and said gel-like material being a substance which has a gel dissolution temperature of 100 degree C or less, solates when heated and is in a gel state at room temperature and has absorbency for a specific wave length of visible and infrared regions, and said light source having a monochromatic light in said specific wave length, wherein said light source is disposed such that it irradiates on said absorption layer and /or said gel layer, with the exception that when no absorption layer is provided, at least two layers each composed of a gel-like material are laminated on said transparent base plate.

摘要翻译： 本发明提供一种处理装置及用于微腔具有所需培养空间的处理方法用于细胞培养。 用于本发明的细胞培养物的微腔室处理设备包括一个微室，其包含不超过一个吸收层和至少一个凝胶层，以该顺序，层叠在不具有显着的吸收性的透明基板 在可见和红外区域，以及至少一个光源，所述具有吸收层在可见和红外区域吸收，并且所述凝胶状材料是其具有100摄氏度或更低，solates当加热和凝胶溶解温度的物质 是在室温下为凝胶状态，并具有吸收可见光区域和红外区域的特定波长，并具有在所述特定波长单色光，所述光源worin所述光源设置检查做到了照射所述吸收层上 和/或所述凝胶层，不同之处做了当没有吸收层被设置，每一个至少两层的凝胶状材料构成的层叠在透明说 NT底板。

公开(公告)号：EP1344817A1

公开(公告)日：2003-09-17

申请号：EP01997537.4

申请日：2001-11-22

申请人： JAPAN SCIENCE AND TECHNOLOGY CORPORATION

发明人： YASUDA, Kenji ,  KANEKO, Kunihiko ,  YOMO, Tetsuya ,  INOUE, Ippei ,  WAKAMOTO, Yuichi ,  MORIGUCHI, Hiroyuki

IPC分类号： C12M1/34 ,  C12M1/00 ,  C12M1/12 ,  C12M1/26 ,  C12M1/38 ,  C12M1/42

CPC分类号： C12M25/02 ,  B01L3/502 ,  C12M25/00 ,  C12M41/36 ,  C12M41/46 ,  G01N35/028 ,  G01N35/1002 ,  G02B21/34

摘要： Novel technical means is provided with a cell culture container having a cell culture region made of a hole formed on a substrate, a semi-permeable membrane covering a top plane of the cell culture region and a culture medium replacement part provided over the semi-permeable membrane, means for supplying a cell culture medium into the cell culture container, and microscopic optical means for enabling long-term observation of the cell within the cell culture region. The novel technical means makes it possible to culture a cell group originating from a particular single cell, to perform culture and observation while identifying cells to be subjected to interaction during the process of culturing cells, to spray a substance which interacts with cells, for example, a drug such as a signal substance, onto only a particular cell in a cell group which is being cultured so that cells are cultured at a constant cell density, and observe a difference in variation between the particular cell and other cells. There is also provided novel means which makes it possible to collect only a cell assuming a particular state and perform analysis or biochemical measurement of a gene of the cell, an expressed mRNA and the like.