公开(公告)号：EP3405788A1

公开(公告)日：2018-11-28

申请号：EP17701827.2

申请日：2017-01-19

Applicant: General Electric Company

Inventor： PULEO, Christopher, Michael ,  KOVACS, Ernest, William ,  DAVIS, Brian, Michael

IPC: G01N33/50 ,  B01L3/00 ,  G01N33/543

CPC classification number: G01N33/54326 ,  B01L3/00 ,  B01L3/50273 ,  B01L3/502753 ,  B01L2200/0668 ,  B01L2200/141 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2400/043 ,  G01N33/505 ,  G01N33/54346 ,  G01N33/56972

Abstract: Described are multi-functional beads and methods to capture rare cells directly from low-volume biological samples and perform both functional and genomic assays from those cells. This is accomplished using a multifunctional capture bead that allows co-localization of both the single cell capture element and the molecular assay components. When combined with a digital microfluidic platform this enables encoding and/or barcoding of specific single cells.

公开(公告)号：EP3383534A1

公开(公告)日：2018-10-10

申请号：EP15804624.3

申请日：2015-11-30

Applicant: Rqmicro AG

Inventor： SCHAFFHAUSER, Daniel ,  KESERUE, Hans-anton ,  AKEPSIMAIDIS, Georgios

IPC: B01L3/00

CPC classification number: B01L3/502761 ,  B01L3/502715 ,  B01L3/502776 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/043 ,  B01L2400/086

Abstract: A microfluidic device (1) comprising, a pallet, having a first surface (4a) and second, opposite, surface (4b); the first surface (4a) having defined therein, a main channel (5), and one or more inlet subsidiary channels (6a,6b) each of which is in fluid communication with the main channel (5) at a first junction (7) which is located at one end of the main channel (5), and corresponding one or more outlet subsidiary channels (8a,8b) each of which is in fluid communication with the main channel (5) at a second junction (9) which is located an second, opposite, end of the main channel (5); wherein the depth (‘d’) of the one or more inlet subsidiary channels (6a,6b) and the depth (‘χ’) of the one or more outlet subsidiary channels (8a,8b) is less than the depth (‘f) of the main channel (5) so that there is step (106a,106b, 108a, 108b) defined at the first junction (7) and at the second junction (9); the second, opposite, surface (4b) having defined therein a groove (15) which can receive a means for generating a magnetic field, wherein the groove (15) is aligned with, and extends parallel to, the main channel (5). There is further provided a corresponding assembly and method of extracting ferromagnetic, paramagnetic and/or diamagnetic particles from a sample.

公开(公告)号：EP2732878B1

公开(公告)日：2018-09-12

申请号：EP12193347.7

申请日：2012-11-20

Applicant: QIAGEN GmbH

Inventor： Focke, Maximilian, Dr. ,  Breitkopf, Lothar ,  Kowalewski, Karen ,  Singer, Thorsten, Dr.

IPC: B01L9/06 ,  G01N35/00

CPC classification number: B01L9/06 ,  B01L2200/028 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/043 ,  B01L2300/0829 ,  B01L2300/12 ,  B01L2400/043 ,  B03C1/0332 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  G01N35/0098

Abstract: Magnetic rack system comprising a holder (1) having trough-holes for receiving tubes (3), a base (4) having a plurality of receptacles (5) with at least one magnet (7), wherein trough-holes in the holder are arranged relative to the base such that each tube can be positioned in a respective receptacle in a pre-determined position relative to the magnet, and an adapter (8), wherein the adapter is designed to allow transmission of motion to the tubes (2) positioned in the holder (1).

公开(公告)号：EP3232203A4

公开(公告)日：2018-07-18

申请号：EP15866519

申请日：2015-12-11

Applicant: PHC HOLDINGS CORP

Inventor： OKAMOTO FUSATOSHI ,  JOHNO MASAHIRO

IPC: G01N35/00 ,  B01L3/00 ,  G01N35/08 ,  G01N35/10 ,  G01N37/00

CPC classification number: G01N35/00069 ,  B01L3/50273 ,  B01L2200/0621 ,  B01L2200/0668 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0406 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0457 ,  G01N35/00 ,  G01N35/00584 ,  G01N35/0098 ,  G01N35/08 ,  G01N35/1016 ,  G01N37/00 ,  G01N2035/00495

Abstract: Provided is a substrate for sample analysis, which involves transportation of a liquid through rotational motion, the substrate for sample analysis including: a substrate including a rotation axis; a first chamber (101), which is positioned in the substrate and includes a first space configured to retain the liquid; a second chamber (102), which is positioned in the substrate and includes a second space configured to retain the liquid discharged from the first chamber; and a first flow passage (111), which is positioned in the substrate, includes a path configured to connect the first chamber and the second chamber to each other, and is capable of being filled with the liquid retained in the first space through a capillary phenomenon, in which the first flow passage (111) has a first opening (111c) and a second opening (111d), the first opening (111c) and the second opening (111d) are connected to the first chamber (101) and the second chamber (102), respectively, and the first opening is positioned on a side closer to the rotation axis (110) than the second opening, in which the first space includes a first region, which is connected to the first opening (111c) and includes a portion extending from the first opening toward a side farther from the rotation axis, and in which the first space of the first chamber (101) has a capacity larger than a capacity of the first flow passage (111).

公开(公告)号：EP3259386A4

公开(公告)日：2018-07-11

申请号：EP16752901

申请日：2016-02-16

Applicant: UNIV MICHIGAN REGENTS

Inventor： CHUNG MENG TING ,  SONG YUJING ,  MCHUGH WALKER M ,  CHEN PENGYU ,  KURABAYASHI KATSUO ,  CORNELL TIMOTHY T ,  SHANLEY THOMAS P

IPC: C40B40/10 ,  B01L3/00 ,  G01N33/543

CPC classification number: G01N33/54373 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0877 ,  B01L2300/123 ,  B01L2300/161 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0442 ,  B01L2400/0457 ,  B01L2400/0487 ,  C40B40/10 ,  G01N33/54346

Abstract: Provided herein are systems and methods for assays. In particular, provided herein are systems and methods for performing high throughput immunoassays. Embodiments of the present disclosure provide multiplex capable LSPR immunoassays that meet a need for rapid (e.g., near real time), accurate immunoassays (e.g. for use in beside diagnostics). The LSPR assays are as accurate as existing ELISA assays but provide the advantage of increased speed and multiplex capability. In addition, the LSPR immunoassays are able to analyze small volumes of complex patient samples (e.g., serum).

公开(公告)号：EP3215271A4

公开(公告)日：2018-06-13

申请号：EP15856708

申请日：2015-11-03

Applicant: MASSACHUSETTS GEN HOSPITAL

Inventor： KAPUR RAVI ,  SMITH KYLE C ,  TONER MEHMET

IPC: G01N35/08 ,  A61K35/28 ,  B01L3/00 ,  C12M1/12 ,  C12N5/0787 ,  G01N15/00 ,  G01N15/02 ,  G01N15/06

CPC classification number: B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493

Abstract: A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

公开(公告)号：EP2561354B1

公开(公告)日：2018-06-06

申请号：EP11772698.4

申请日：2011-04-21

Applicant: DNAE Group Holdings Limited

Inventor： ESCH, Victor, C. ,  DRYGA, Sergey, A. ,  CLARIZIA, Lisa-jo, Ann

IPC: G01N33/48

CPC classification number: G01N33/54326 ,  A61B5/055 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2400/043 ,  C07K1/22 ,  C07K16/1267 ,  C12N13/00 ,  C12N15/1013 ,  C12Q1/6806 ,  C12Q1/689 ,  C12Q2565/633 ,  G01N24/08 ,  G01N33/54333 ,  G01N33/56911 ,  G01N2333/195 ,  G01N2446/20 ,  G01R33/465 ,  Y10T428/2982 ,  C12Q2565/629 ,  C12Q2563/143

Abstract: The invention generally relates to using magnetic particles and alternating magnet fields to separate a target analyte from a sample. In certain embodiments, methods of the invention involve contacting a sample with magnetic particles including first moieties specific for a target analyte, thereby forming target/particle complexes in the sample, flowing the sample through a channel including second moieties attached to at least one surface of the channel, applying alternating magnetic fields to the flowing sample to result in target/particle complexes being brought into proximity of the surface to bind the second moieties and unbound particles remaining free in the sample, binding the target/particle complexes to the second moieties, and washing away unbound particles and unbound analytes of the sample.

公开(公告)号：EP2149610B1

公开(公告)日：2018-05-16

申请号：EP07730404.6

申请日：2007-03-26

Applicant: Fundacion Gaiker ,  Ikerlan

Inventor： VERDOY BERASTEGUI, Dolores ,  OLABARRIA DE PABLO, Garbiñe ,  RUANO LÓPEZ, Jesús, Miguel ,  BERGANZO RUIZ, Javier

IPC: C12Q1/68 ,  B01J19/00 ,  G01N33/50 ,  B01L3/00 ,  B01L7/00

CPC classification number: B01L7/52 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/14 ,  B01L2300/1827 ,  B01L2400/043 ,  B01L2400/0487

Abstract: The present invention relates to a method and a portable device to detect a genetic material in a biological sample using the technique known as PCR (Polymerase Chain Reaction). The device comprises a reaction chamber which incorporates means of heating arranged to heat said chamber. The detection method is characterized in that the main phases are performed in the reaction chamber. The miniaturized system has the object of increasing efficiency, simplicity of use and the portability of the PCR in comparison with laboratory scale analysis.

公开(公告)号：EP3270141A3

公开(公告)日：2018-05-02

申请号：EP17187723.6

申请日：2012-03-07

Applicant: Université Laval ,  Genepoc Inc.

Inventor： PEYTAVI, Régis ,  CHAPDELAINE, Sébastien

IPC: G01N21/07 ,  G01N35/00 ,  B01L3/00 ,  B01F15/02 ,  B01L9/00 ,  C12Q1/68 ,  G01N21/03 ,  G01N21/64 ,  G01N35/04 ,  B01F13/00

CPC classification number: B01L3/50273 ,  B01F3/0861 ,  B01F13/0059 ,  B01F15/0212 ,  B01F15/0233 ,  B01F2215/0037 ,  B01L3/502715 ,  B01L3/502746 ,  B01L7/525 ,  B01L9/527 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2200/0605 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/147 ,  B01L2200/16 ,  B01L2300/023 ,  B01L2300/043 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/069 ,  B01L2300/0803 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/18 ,  B01L2400/0409 ,  B01L2400/043 ,  B01L2400/0677 ,  B01L2400/0683 ,  B01L2400/082 ,  C12Q1/686 ,  G01N21/0332 ,  G01N21/07 ,  G01N21/645 ,  G01N35/00029 ,  G01N35/00871 ,  G01N2021/0325 ,  G01N2035/00158 ,  G01N2035/00495 ,  G01N2035/0091 ,  G01N2035/0451

Abstract: A fluidic centripetal apparatus (1) for testing components of a biological material in a fluid, said fluidic centripetal device having a shape adapted to be received within a rotatable holder, said rotatable holder having a center of rotation and an outer edge. The apparatus comprising: a fluidic component layer (3) having fluidic features on at least a front face, said fluidic features including an entry channel for circulating said fluid, said entry channel (302) being coupled to a intake (5) receptacle outlet; a retention chamber (403), said retention chamber being coupled to said entry channel via said intake receptacle outlet for receiving said fluid into said retention chamber; a container wholly provided in said retention chamber and containing a liquid diluent, and a bottom component layer (4) bonded to a rear of said fluidic component layer thereby creating a fluidic network through which said fluid flows under centripetal force.

公开(公告)号：EP2515121A4

公开(公告)日：2018-04-04

申请号：EP10837485

申请日：2010-12-08

Applicant: HITACHI HIGH TECH CORP

Inventor： UEMATSU CHIHIRO ,  MAESHIMA MUNEO

IPC: B01L3/02 ,  C12M1/00 ,  C12N15/09 ,  G01N35/10

CPC classification number: G01N35/10 ,  B01L3/0275 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/0829 ,  B01L2400/043 ,  G01N35/0098 ,  G01N2035/1048 ,  Y10T436/25

Abstract: This invention concerns a sample processing device capable of efficiently recovering biological molecules, such as nucleic acids or proteins. The sample processing device is capable of placing a reaction container having a plurality of reaction sites, and it comprises a nozzle mechanism with a nozzle capable of attaching and removing a dispenser tip for dispensing a solution into the reaction sites of the reaction container and a magnetic tip for generating a magnetic field that allows magnetic beads to migrate to a space among the plurality of reaction sites in the reaction container, and a drive control unit controlling the nozzle mechanism.