公开(公告)号：EP0823477A1

公开(公告)日：1998-02-11

申请号：EP97904587.9

申请日：1997-02-20

申请人： Japan Tobacco Inc.

发明人： UEKI, Jun, Japan Tobacco Inc., Plant Breed. ,  MORIOKA, Shinji, Japan Tobacco Inc.

IPC分类号： C12N15/11 ,  C12N15/55 ,  C12N5/10 ,  C12N9/16 ,  A01H1/00 ,  A01H5/00

CPC分类号： C12N15/1137 ,  C12N9/16 ,  C12N15/8247 ,  C12N2310/111 ,  C12Y301/04004

摘要： A method for artificially changing the composition of phospholipids produced by cells is disclosed. According to the method of the invention, the host cell is transformed with a recombinant DNA having an antisense gene of phospholipase D gene, which antisense gene is expressed in the host cell to produce a mRNA that hybridizes with mRNA of phospholipase D gene in the host cell thereby inhibiting expression of the phospholipase D gene.

摘要翻译： 一种用于人工改变由细胞产生的磷脂的组合物的方法是游离缺失盘。 。根据本发明的方法中，所述宿主细胞转化了具有的磷脂酶D基因的反义基因，该基因的反义基因在宿主细胞中过表达，以产生的mRNA做了重组DNA与磷脂酶D基因的mRNA杂交在宿主 从而细胞抑制磷脂酶D基因的表达。