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公开(公告)号：EP2142673B1
公开(公告)日：2013-05-22
申请号：EP08733324.1
申请日：2008-04-04
发明人： TODD, Alison, Velyian , MOKANY, Elisa , DOAN, Tram, Bich , YOUNG, Paul, Ean , SWAIN, Helen, Anne
CPC分类号： C12Q1/6811 , C12N15/111 , C12N2310/127 , C12N2320/11 , C12Q1/6816 , G01N2333/9005 , C12Q2565/1015 , C12Q2537/149 , C12Q2537/143 , C12Q2521/501 , C12Q2521/337 , C12Q2521/301 , C12Q2537/163 , C12Q2537/162
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2.发明公开
公开(公告)号：EP1960554A1
公开(公告)日：2008-08-27
申请号：EP06841303.8
申请日：2006-12-07
发明人： RIMSKY, Laurence Tatiana , DE BAERE, Inky Paul Madeleine , MAES, Bart Anna Julien , DE BETHUNE, Marie-Pierre T.M.M.G , KRAUS, Guenter , MOKANY, Elisa , TODD, Alison Velyian
IPC分类号： C12Q1/70
CPC分类号： C12Q1/703
摘要： The present invention relates to methods and means for the evaluation of HIV replicat ive capacity in a given environment. In particular, the invention provides a growth competition assay that can determine relative viral fitness using a recombinant tagged HIV-1 virus system. The methods rely on plasmid vectors, amplicons, primers and probes, and the generation of replication-competent viruses therefrom. Said methods and materials may find use in multiple fields including diagnostics, drug screening, pharmacogenetics and drug development.
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公开(公告)号：EP1948822B1
公开(公告)日：2011-07-13
申请号：EP06790343.5
申请日：2006-10-06
CPC分类号： C07H21/04 , C12N15/111 , C12N2310/12 , C12N2320/10 , C12N2330/30 , C12Q1/6811 , C12Q1/6818 , C12Q1/682 , C12Q1/6823 , C12Q2537/125 , C12Q2525/161 , C12Q2521/337 , C12Q2525/205
摘要： The present invention relates to Multicomponent Nucleic Acid Enzymes (MNAzymes) and methods for their use. MNAzymes comprise two or more oligonucleotide components which self-assemble in the presence of one or more MNAzyme assembly facilitator molecules to form a catalytically active structure. Compositions for making MNAzymes, and collections of MNAzymes are provided. Also provided are methods for using MNAzymes for the detection, identification and/or quantification of one or more targets. The methods can be practiced in solution-based assays or in assays where one or more reaction components are attached to a support structure. The methods allow for multiplexing the MNAzyme detection to detect multiple targets in a single reaction. Also provided are kits for making the compositions, and for practicing the methods provided herein.
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4.发明授权
公开(公告)号：EP1960554B1
公开(公告)日：2011-06-08
申请号：EP06841303.8
申请日：2006-12-07
发明人： RIMSKY, Laurence Tatiana , DE BAERE, Inky Paul Madeleine , MAES, Bart Anna Julien , DE BETHUNE, Marie-Pierre T.M.M.G , KRAUS, Guenter , MOKANY, Elisa , TODD, Alison Velyian
IPC分类号： C12Q1/70
CPC分类号： C12Q1/703
摘要： The present invention relates to methods and means for the evaluation of HIV replicat ive capacity in a given environment. In particular, the invention provides a growth competition assay that can determine relative viral fitness using a recombinant tagged HIV-1 virus system. The methods rely on plasmid vectors, amplicons, primers and probes, and the generation of replication-competent viruses therefrom. Said methods and materials may find use in multiple fields including diagnostics, drug screening, pharmacogenetics and drug development.
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公开(公告)号：EP1948822A1
公开(公告)日：2008-07-30
申请号：EP06790343.5
申请日：2006-10-06
CPC分类号： C07H21/04 , C12N15/111 , C12N2310/12 , C12N2320/10 , C12N2330/30 , C12Q1/6811 , C12Q1/6818 , C12Q1/682 , C12Q1/6823 , C12Q2537/125 , C12Q2525/161 , C12Q2521/337 , C12Q2525/205
摘要： The present invention relates to Multicomponent Nucleic Acid Enzymes (MNAzymes) and methods for their use. MNAzymes comprise two or more oligonucleotide components which self-assemble in the presence of one or more MNAzyme assembly facilitator molecules to form a catalytically active structure. Compositions for making MNAzymes, and collections of MNAzymes are provided. Also provided are methods for using MNAzymes for the detection, identification and/or quantification of one or more targets. The methods can be practiced in solution-based assays or in assays where one or more reaction components are attached to a support structure. The methods allow for multiplexing the MNAzyme detection to detect multiple targets in a single reaction. Also provided are kits for making the compositions, and for practicing the methods provided herein.
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公开(公告)号：EP2142673A1
公开(公告)日：2010-01-13
申请号：EP08733324.1
申请日：2008-04-04
发明人： TODD, Alison, Velyian , MOKANY, Elisa , DOAN, Tram, Bich , YOUNG, Paul, Ean , SWAIN, Helen, Anne
CPC分类号： C12Q1/6811 , C12N15/111 , C12N2310/127 , C12N2320/11 , C12Q1/6816 , G01N2333/9005 , C12Q2565/1015 , C12Q2537/149 , C12Q2537/143 , C12Q2521/501 , C12Q2521/337 , C12Q2521/301 , C12Q2537/163 , C12Q2537/162
摘要： The present invention relates to methods that utilise multi-component nucleic acid complex (MNA complex) cascades. The MNA complexes may have cleavage or ligase activity. Further, the invention provides cascades which may include one or more DNAzymes. The invention also provides methods which use these cascades for the identification, detection and quantification of targets.
摘要翻译： 本发明涉及利用多组分核酸复合物（MNA复合物）级联的方法。 MNA复合物可能具有切割或连接酶活性。 此外，本发明提供了可包含一种或多种脱氧核酶的级联。 本发明还提供了使用这些级联来识别，检测和定量目标的方法。
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公开(公告)号：EP2066817A1
公开(公告)日：2009-06-10
申请号：EP07815323.6
申请日：2007-10-05
发明人： TODD, Alison, Velyian , MOKANY, Elisa , BIRKETT, Donald, John , DOAN, Tram, Bich , REID, Christopher, Roland
IPC分类号： C12Q1/70
CPC分类号： C12Q1/6818 , C12Q1/682 , Y02P20/582 , C12Q2565/1015 , C12Q2537/125 , C12Q2521/337 , C12Q2521/501
摘要： The present invention relates to compositions and methods that allow the manipulation of the catalytic activity of multi-component nucleic acid complexes. Further, the invention provides methods which use these compositions and methods to create molecular sensors, molecular switches, and/or modulators or propagators of autocatalytic self-replicating cascades and other iterative processes. More particularly, the invention relates to compositions allowing self-assembly of active and inactive multicomponent nucleic acid complexes, methods of making such compositions, and methods for use.
摘要翻译： 本发明涉及允许操纵多组分核酸复合物的催化活性的组合物和方法。 此外，本发明提供了使用这些组合物和方法来创建分子传感器，分子开关和/或自催化自复制级联和其他迭代过程的调制器或传播器的方法。 更具体地说，本发明涉及允许活性和失活多组分核酸复合物自组装的组合物，制备这种组合物的方法以及使用方法。
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