公开(公告)号：EP1602720B1

公开(公告)日：2010-11-03

申请号：EP04713206.3

申请日：2004-02-20

申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute

发明人： KOYANAGI, Satoshi, Chemo-sero-therap. res.inst. ,  SUGAWARA, Keishin, Chemo-sero-therap. res.inst. ,  MIYATSU, Yoshinobu, Chemo-sero-therap. res. inst. ,  MURAKAMI, Toshio, Chemo-sero-therap. research inst ,  MAEDA, Toshihiro, Chemo-sero-therapeut. res.inst. ,  MIZOKAMI, Hiroshi, Chemo-sero-therap. res.inst.

IPC分类号： C12N15/09 ,  C12P21/02 ,  C07K14/435

CPC分类号： C07K14/43531 ,  C12N15/70 ,  C12P21/02

摘要： It is intended to provide a foreign protein free from any expression inducer and a process for producing the same. Namely, a process for producing a foreign protein involving the step of proliferating a recombinant Escherichia coli, which expresses the foreign protein under the control by a promoter capable of inducing the expression due to the temperature shift, by arbitrarily culturing at a low temperature and then culturing the strain at a high temperature in the absence of an expression inducer to thereby allow the expression of the foreign protein, or the step of culturing the above Escherichia coli strain at a high temperature and thus effecting the proliferation of the cells and the expression of the foreign protein at the same time; and a foreign protein free from any expression inducer which is obtained by this process. Particular examples of such foreign proteins include main tick allergens, Actinogacillus pleuropneumoniae-origin secretory macrophage toxin and a protective antigen against Erysipelothrix rhusiopathiae-infection.

公开(公告)号：EP1602720A1

公开(公告)日：2005-12-07

申请号：EP04713206.3

申请日：2004-02-20

申请人： Juridical Foundation, The Chemo-Sero-Therapeutic Research Institute

发明人： KOYANAGI, Satoshi, Chemo-sero-therap. res.inst. ,  SUGAWARA, Keishin, Chemo-sero-therap. res.inst. ,  MIYATSU, Yoshinobu, Chemo-sero-therap. res. inst. ,  MURAKAMI, Toshio, Chemo-sero-therap. research inst ,  MAEDA, Toshihiro, Chemo-sero-therapeut. res.inst. ,  MIZOKAMI, Hiroshi, Chemo-sero-therap. res.inst.

IPC分类号： C12N15/09 ,  C12P21/02

CPC分类号： C07K14/43531 ,  C12N15/70 ,  C12P21/02

摘要： A heterologous protein free from an inducer and a method for producing said protein are provided. A method for producing a heterologous protein which comprises the step of optionally culturing at low temperature recombinant E. coli cells expressing a heterologous protein under control of a promoter capable of inducing expression through temperature shift and then culturing at high temperature said recombinant E. coli cells in the absence of an inducer to thereby allow for expression of said heterologous protein, or the step of culturing at high temperature said recombinant E. coli cells to thereby simultaneously allow for both cell proliferation and expression of said heterologous protein, and the heterologous protein obtained by said method that is free from an inducer. Such heterologous protein may include a major mite allergen, a secretary macrophage toxin from Actinobacillus pleuropneumoniae and a surface protective antigen (SpaA) of Erysipelothrix rhusiopathiae.

摘要翻译： 提供了不含诱导物的异源蛋白质和生产所述蛋白质的方法。 一种产生异源蛋白质的方法，其包括在低温下表达异源蛋白质的重组大肠杆菌细胞在能够通过温度转移诱导表达，然后在高温下培养的启动子的控制下培养的步骤，所述重组大肠杆菌细胞 在不存在诱导物从而允许所述异源蛋白质的表达的情况下，或在高温下培养所述重组大肠杆菌细胞从而同时允许所述异源蛋白质的细胞增殖和表达的步骤，并且获得的异源蛋白质 通过所述方法不含诱导剂。 这种异源蛋白质可以包括主要的螨变应原，来自胸膜肺炎放线杆菌的秘密巨噬细胞毒素和Erysipelothrix rhusiopathiae的表面保护性抗原（SpaA）。