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11 条记录 (耗时 0.007 秒)

    MODIFIED AMADORIASE CAPABLE OF ACTING ON FRUCTOSYL HEXAPEPTIDE
    1.
    发明公开
    MODIFIED AMADORIASE CAPABLE OF ACTING ON FRUCTOSYL HEXAPEPTIDE 审中-公开
    ZUR WIRKUNG AUF FRUCTOSYLHEXAPEPTIDFÄHIGE，MODIFIZIERTE AMADORIASE

    公开(公告)号：EP2843050A4

    公开(公告)日：2016-03-02

    申请号：EP13781492

    申请日：2013-04-26

    申请人： KIKKOMAN CORP

    发明人： ICHIYANAGI ATSUSHI ,  MASAKARI YOSUKE

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/10 ,  C12Q1/48

    CPC分类号： C12Q1/26 ,  C12N9/0032 ,  G01N33/723 ,  G01N2333/90672

    摘要： This invention provides an amadoriase having high reactivity with ±-fructosyl hexapeptide (±F6P) derived from the ²-chain amino terminus of glycated hemoglobin (HbAlc), which enables satisfactory quantification of ±F6P cleaved from HbAlc. A novel amadoriase is derived from the amadoriase of the genus Coniochaeta by substitution of one or more amino acids at positions selected from the group consisting of positions 62, 63, 102, 106, 110, 113, 355, and 419. Such amadoriase enables rapid, simple, accurate, and satisfactory quantification of ±F6P cleaved from HbAlc. With the use of such amadoriase, a method for measurement of HbAlc by an enzymatic method and a kit for measurement of HbAlc that are based on the same principle as used in the conventional standard method and excellent in terms of the relevance with the standard method.

    摘要翻译： 本发明提供了一种与衍生自糖基化血红蛋白（HbAlc）的2-链氨基末端的± - 果糖基六肽（±F6P）具有高反应性的阿马替利酶，其能够令人满意地定量从HbAlc切割的±F6P。 一种新的阿马道里酶通过在选自位置62,63,102,106,110,113,355和419的位置取代一个或多个氨基酸衍生自Coniochaeta属的阿马道里酶。这种阿马道里酶能够快速 ，从HbAlc切割的±F6P的简单，准确和令人满意的定量。 使用这种阿马道里酶，通过酶法测定HbAlc的方法和用于测量HbAlc的试剂盒，其基于与常规标准方法中使用的相同原理，并且与标准方法的相关性优异。

    MODIFIED AMADORIASE AND METHOD FOR PRODUCING SAME, AGENT FOR IMPROVING SURFACTANT-RESISTANCE OF AMADORIASE AND COMPOSITION FOR MEASURING HbA1c USING SAME
    2.
    发明公开
    MODIFIED AMADORIASE AND METHOD FOR PRODUCING SAME, AGENT FOR IMPROVING SURFACTANT-RESISTANCE OF AMADORIASE AND COMPOSITION FOR MEASURING HbA1c USING SAME 审中-公开
    改性阿马AND METHOD FOR PRODUCING物的活性，以改善表面活性剂抗性阿马和组合物因此测量HBA1

    公开(公告)号：EP3031914A4

    公开(公告)日：2017-01-11

    申请号：EP14834684

    申请日：2014-08-08

    申请人： KIKKOMAN CORP

    发明人： MASAKARI YOSUKE ,  ICHIYANAGI ATSUSHI

    IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N9/06 ,  C12Q1/26 ,  G01N33/72

    CPC分类号： C12Q1/26 ,  C12N9/0032 ,  C12Y105/03 ,  G01N33/723 ,  G01N2333/805 ,  G01N2333/90672

    摘要： Provided is a composition by which glycated hemoglobin can be measured even in the presence of a stronger surfactant than a conventional case. Also provided is a buffer and/or stabilizer which maintains the residual activity of an amadoriase or lowers a reduction of residual activity. The present invention provides a composition for use in measuring glycated hemoglobin containing an amadoriase having substitution of one or more amino acid residues at a position(s) corresponding to an amino acid(s) selected from the group consisting of position 262, position 257, position 249, position 253, position 337, position 340, position 232, position 129, position 132, position 133, position 44, position 256, position 231 and position 81 of an amadoriase derived from the genus Coniochaeta and represented by SEQ ID No: 1 or 3, and having residual activity even in the presence of a surfactant. The present invention also provides a composition and kit for use in measuring glycated hemoglobin, comprising a specific stabilizer and/or a buffer. The present invention can provide an enzyme and a composition for use in measuring glycated hemoglobin, excellent in storage stability even if they are exposed to a surfactant.

    摘要翻译： 提供了通过该糖化血红蛋白甚至可以在表面活性剂比常规情况下更强的存在下测得的组合物。 如此提供的是其保持阿马道里的残余活性或降低的降低残留活性的缓冲液和/或稳定剂。 本发明提供了在测量糖化血红蛋白在阿马含有具有一个或多个氨基酸残基取代在在选自262位选择的氨基酸（S）对应于位置（S），位置257中使用的组合物， 位置249，位置253，位置337，位置340，位置232，位置129，位置132，位置133，位置44，位置256，位置231和从该属锥毛壳衍生和由SEQ ID NO的阿马道里酶的位置81： 1或3，并且具有即使在表面活性剂存在的残余活性。 本发明因此提供了在测量糖化血红蛋白中使用的组合物和试剂盒，包括一个特定的稳定剂和/或缓冲剂。 本发明可以提供酶和测量糖化血红蛋白，具有优异的储存稳定性，即使它们被暴露到表面活性剂中使用的组合物。

    HEMOGLOBIN A1c MEASUREMENT METHOD AND MEASUREMENT KIT
    4.
    发明公开
    HEMOGLOBIN A1c MEASUREMENT METHOD AND MEASUREMENT KIT 审中-公开
    HÄMOGLOBIN-A1C-MESSVERFAHREN UND -MESSKIT

    公开(公告)号：EP3061819A4

    公开(公告)日：2017-08-16

    申请号：EP14856134

    申请日：2014-10-24

    申请人： KIKKOMAN CORP

    发明人： MASAKARI YOSUKE ,  ICHIYANAGI ATSUSHI

    IPC分类号： C12N15/09 ,  C12N9/06 ,  C12Q1/26 ,  C12Q1/28

    CPC分类号： C12Q1/26 ,  C12N9/0032 ,  C12Q1/28 ,  C12Y105/03 ,  C12Y111/01 ,  G01N21/78 ,  G01N33/725 ,  G01N2333/805 ,  G01N2333/90672 ,  G01N2333/908

    摘要： This invention provides an amadoriase that acts on the ²-chain of hemoglobin A1c (HbA1c) and generates hydrogen peroxide, a method for measurement of HbA1c using such amadoriase, and a reagent kit for measurement of HbAlc using such amadoriase. The method for measurement of HbAlc involves the use of an amadoriase that has specific activity of 0.1 U/mg or greater to ±F6P and oxidizes the HbA1c ²-chain amino terminus so as to generate hydrogen peroxide, and the reagent kit for measurement of HbA1c comprises such amadoriase. The method and the kit for measurement of HbAlc enable quantification of HbAlc to be performed rapidly, simply, and accurately.

    摘要翻译： 本发明提供一种作用于血红蛋白A1c（HbA1c）的双链并产生过氧化氢的阿马多里酶，使用这种阿马多里酶测定HbA1c的方法和使用该阿马多里酶测定HbA1c的试剂盒。 测定HbA1c的方法包括使用对F6P具有0.1U / mg或更高比活性的阿马多里酶并氧化HbA1c2链氨基末端以产生过氧化氢，以及用于测量HbA1c的试剂盒 包括这种阿马多里酶。 用于测量HbA1c的方法和试剂盒能够快速，简单且准确地进行HbAlc的定量。

    HbA1c MEASUREMENT METHOD USING AMADORIASE ACTING ON GLYCATED PEPTIDES
    5.
    发明公开
    HbA1c MEASUREMENT METHOD USING AMADORIASE ACTING ON GLYCATED PEPTIDES 审中-公开
    随着对糖基化肽署理阿马A1C测量方法

    公开(公告)号：EP3061829A4

    公开(公告)日：2017-04-19

    申请号：EP14856377

    申请日：2014-10-24

    申请人： KIKKOMAN CORP

    发明人： ICHIYANAGI ATSUSHI

    IPC分类号： C12Q1/26 ,  C12M1/34 ,  C12N9/06 ,  C12N15/09 ,  C12Q1/37

    CPC分类号： G01N33/723 ,  C07K14/37 ,  C12N9/0032 ,  G01N2333/805 ,  G01N2333/90672

    摘要： This invention provides an amadoriase that can react with a wide variety of glycated peptides generated upon hydrolysis of the ² chain of hemoglobin A1c (HbA1c) to generate hydrogen peroxide, a method for measurement of HbAlc using such amadoriase, and a reagent kit for measurement of HbAlc using such amadoriase. Provided is an amadoriase obtained by substitution of one or more amino acids at positions corresponding to amino acids selected from the group consisting of amino acids 62, 63, 102, 106, 110, 113, and 355 in the amino acid sequence of the amadoriase derived from the genus Coniochaeta or the like which amadoriase is capable of oxidizing a wide variety of glycated peptides to generate hydrogen peroxide. Further provided is a method for measurement of HbAlc comprising using such amadorise as well as a reagent kit for measurement of HbAlc comprising such amadoriase. This invention can provide a method for measurement of HbAlc that enables quantification of HbAlc to be performed rapidly, simply, and accurately with the use of a small amount of a protease and a kit used for such measurement. This invention can also provide a method for measurement of HbAlc that enables quantification of HbAlc to be performed rapidly, simply, and accurately, with high sensitivity with the use of a small amount of a protease and a kit used for such measurement.