公开(公告)号：EP1041151A1

公开(公告)日：2000-10-04

申请号：EP98961523.2

申请日：1998-12-24

申请人： KIKKOMAN CORPORATION

发明人： HATTORI, Noriaki Kikkoman Corporation ,  MURAKAMI, Seiji, Kikkoman Corporation

IPC分类号： C12N15/53 ,  C12N9/02 ,  C12N15/63 ,  C12N1/21 ,  C12Q1/26

CPC分类号： C12N9/0069 ,  C12Q1/66

摘要： The present invention relates to luciferase having resistance to a surfactant and a method for measuring intracellular ATP which is characterized in that the luciferase having resistance to a surfactant is used in this method comprising the steps of: a first step wherein ATP is extracted from cells in a sample; a second step wherein light emission is produced by adding a luminescence reagent containing luciferase to the extracted ATP solution; and a third step wherein the light emission is measured.

摘要翻译： 本发明涉及荧光素酶具有与表面活性剂和用于测量细胞内ATP的所有这是在没有具有与表面活性剂抗性的荧光素酶在该方法中，包括以下步骤中使用为特征的方法电阻：worin ATP第一步骤是从细胞中提取 的样品; 第二步骤worin光发射是通过添加发光试剂的萤光素酶含有与ATP萃取溶液制备; 和第三步骤worin发光进行测量。

公开(公告)号：EP1041151B1

公开(公告)日：2007-09-12

申请号：EP98961523.2

申请日：1998-12-24

申请人： KIKKOMAN CORPORATION

发明人： HATTORI, Noriaki Kikkoman Corporation ,  MURAKAMI, Seiji, Kikkoman Corporation

IPC分类号： C12N15/53 ,  C12N9/02 ,  C12N15/63 ,  C12N1/21 ,  C12Q1/26

CPC分类号： C12N9/0069 ,  C12Q1/66

摘要： A luciferase tolerant to surfactants; and a method for assaying intracellular ATP which comprises the first step of extracting ATP from a cell-containing sample in the presence of a surfactant, the second step of adding a luciferase-containing luminescent reagent to the ATP extract thus inducing luminescence and the third step of detecting the luminescence dose, characterized by using the luciferase tolerant to surfactants.

公开(公告)号：EP1048739B1

公开(公告)日：2005-09-07

申请号：EP98956015.6

申请日：1998-12-01

申请人： KIKKOMAN CORPORATION

发明人： HATTORI, Noriaki, Kikkoman Corporation ,  YAJITATE, Keiko, Kikkoman Corporation ,  NAKAJIMA, Motoo, Kikkoman Corporation ,  MURAKAMI, Seiji, Kikkoman Corporation

IPC分类号： C12Q1/66

CPC分类号： G01N33/66 ,  C12Q1/66 ,  C12Q2304/60 ,  G01N2400/16 ,  G01N2400/18

摘要： A method for analyzing intracellular components characterized by involving the following steps; and kits containing (a) an extractant, (b) a branched dextrin or its derivative, and (c) a reagent for analyzing intracellular components: (1) the step of adding the extractant to a sample containing cells to thereby extract intracellular components; (2) the step of adding the branched dextrin or its derivative to the sample containing the extractant; and (3) the step of analyzing the thus extracted intracellular components. The use of the branched dextrin or its derivative makes it possible to prevent the extractant from inhibiting the analysis steps.