摘要:
To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.
摘要:
A method for producing luciferase which comprises culturing in a medium a luciferase-producing microorganism belonging to the genus Escherichia and containing a recombinant DNA having incorporated into a vector DNA a luciferase gene encoding an amino acid sequence shown in Fig. 4, and collecting luciferase from the culture.
摘要:
A luciferase gene derived from Lucida cruciata and a novel recombinant DNA characterized by incorporating a gene coding for luciferase into a vector DNA are disclosed. There is also disclosed a method of producing luciferase which comprises culturing in a medium a microorganism containing a recombinant DNA having inserted a gene coding for luciferase in a vector DNA and belonging to the genus Escherichia capable of producing luciferase and collecting luciferase from the culture.