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1.发明公开GENOME-WIDE CONSTRUCTION OF SCHIZOSACCHAROMYCES POMBE HETEROZYGOUS DELETION MUTANTS CONTAINING GENE-SPECIFIC BARCODES BY THE METHODS OF 4-ROUND SERIAL OR BLOCK PCR, OR TOTAL GENE SYNTHESIS THEREOF 审中-公开全基因组构建的基因特异性条形码CONTAINING粟酒裂殖酵母杂合缺失突变体以4轮的串联或嵌段PCR OR GESAMTGENSYNTHESE THEREOF过程
公开(公告)号：EP2268808A4
公开(公告)日：2011-09-14
申请号：EP08793534
申请日：2008-08-27
发明人： HOE KWANG LAE , KIM DONG UK , WON MI SUN , YOO HYANG SOOK , KIM DONG SUP , PARK HAN OH , CHUNG KYUNG SOOK , JANG YOUNG JOO , NAM MI YOUNG , HAN SANG JO , CHOI SHIN JUNG , BAEK SEUNG TAE , KIM HYONG BAI , HEO KYUNG SUN , LEE HYE MI , LEE MIN HO , PARK JO YOUNG
IPC分类号： C12N15/00
CPC分类号： C12N15/1034 , C12N1/00 , C40B30/00 , C40B40/02 , C40B50/00
摘要： A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.
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