公开(公告)号：EP1686173A1

公开(公告)日：2006-08-02

申请号：EP04773766.3

申请日：2004-10-08

申请人： KYOWA HAKKO KOGYO CO., LTD.

发明人： NAKANO, Ryosuke, c/o Kyowa Hakko Kogyo Co.,Ltd. ,  SATOH, Mitsuo, c/o Kyowa Hakko Kogyo Co.,Ltd. ,  IIDA, Shigeru, c/o Kyowa Hakko Kogyo Co.,Ltd. ,  INOUE, Miho, c/o Kyowa Hakko Kogyo Co.,Ltd. ,  KUSUNOKI, Machi, c/o Kyowa Hakko Kogyo Co.,Ltd. ,  KINOSHITA, Satoko, c/o Kyowa Hakko Kogyo Co.,Ltd. ,  OHNUKI, Naoko, c/o Kyowa Hakko Kogyo Co.,Ltd.

IPC分类号： C12N5/10 ,  C12N15/13 ,  C12P21/08

CPC分类号： C12N5/0018 ,  C12N2500/92 ,  C12N2500/95 ,  C12N2510/00 ,  C12N2510/02

摘要： Development of a host cell capable of producing a glycoprotein composition such as an antibody composition which is useful in development of medicaments is desired.
The present invention provides a cell in which a genomic gene encoding an enzyme relating to a sugar chain modification in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α-bond in a complex type N-glycoside-linked sugar chain is knocked out, wherein the cell is naturalized in a serum-free medium and a process for producing a glycoprotein composition using the cell.

摘要翻译： 需要开发能够产生可用于开发药物的糖蛋白组合物如抗体组合物的宿主细胞。 本发明提供一种细胞，其中编码与糖链修饰有关的酶的基因组基因，其中岩藻糖的1位与还原末端的N-乙酰葡糖胺的6-位在复合型N中结合 糖苷连接的糖链被敲除，其中细胞在无血清培养基中归巢，以及使用该细胞产生糖蛋白组合物的方法。