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    NUCLEIC ACID ANALYSIS METHOD
    1.
    发明公开
    NUCLEIC ACID ANALYSIS METHOD 审中-公开
    VERFAHRENFÜRNUKLEINSÄUREANALYSE

    公开(公告)号：EP2740804A1

    公开(公告)日：2014-06-11

    申请号：EP12822928.3

    申请日：2012-07-27

    申请人： Kabushiki Kaisha Toshiba

    发明人： NAKAMURA, Naoko ,  HASHIMOTO, Koji ,  HASHIMOTO, Michie ,  GEMMA, Nobuhiro ,  NIKAIDO, Masaru

    IPC分类号： C12Q1/68 ,  C12N15/09

    CPC分类号： C12Q1/6837 ,  C12Q1/6853 ,  C12Q2525/301

    摘要： Embodiments relate to a method of analyzing nucleic acid. The nucleic acid analysis method includes subjecting a sample to an amplifying reaction using the first primer and the second primer, reacting an amplification product with a nucleic acid probe, measuring presence/absence of a hybridization and/or a quantity thereof, and determining presence/absence of a target nucleic acid in the sample and/or a quantity of target nucleic acid present. The amplification product obtained with the first primer and/or second primer forms a first stem-and-loop structural body. With the detection of the presence/absence of the hybridization between a nucleic acid probe complementary to a sequence of the loop structure and the amplification product, and/or the amount thereof present, the nucleic acid analysis is carried out on the sample.

    摘要翻译： 实施方案涉及分析核酸的方法。 核酸分析方法包括使用第一引物和第二引物对扩增反应进行扩增反应，使扩增产物与核酸探针反应，测定杂交的存在/不存在和/或其量，并确定存在/ 不存在样品中的靶核酸和/或存在的目标核酸的量。 用第一底漆和/或第二底漆获得的扩增产物形成第一茎 - 环结构体。 通过检测与环结构序列互补的核酸探针和扩增产物之间的杂交存在/不存在和/或其存在量，对样品进行核酸分析。

    NUCLEIC ACID DETECTION CASSETTE AND NUCLEIC ACID DETECTION APPARATUS
    2.
    发明公开
    NUCLEIC ACID DETECTION CASSETTE AND NUCLEIC ACID DETECTION APPARATUS 审中-公开
    NUKLEINSÄUREDETEKTIONSKASSETTEUNDNUKLEINSÄUREDETEKTIONSVORRICHTUNG

    公开(公告)号：EP1997888A1

    公开(公告)日：2008-12-03

    申请号：EP07739538.2

    申请日：2007-03-23

    申请人： Kabushiki Kaisha Toshiba

    发明人： HONGO, Sadato ,  OKADA, Jun ,  OOKI, Kenji ,  GEMMA, Nobuhiro

    IPC分类号： C12N15/09 ,  C12M1/00 ,  C12Q1/68 ,  G01N27/28 ,  G01N27/327 ,  G01N35/08 ,  G01N37/00

    CPC分类号： G01N35/1095 ,  G01N33/5308 ,  G01N33/54366

    摘要： A nucleic acid cassette includes a main body in which first and second channels are formed. A sample containing a target nucleic acid is input in a sample chamber of the second channel via an injection port. In the cassette, a detecting part is incorporated which includes a detection chamber communicated to the first and second channels and a nucleic detection unit. The first channel has a first reagent reservoir storing a first reagent stored and a second reagent reservoir storing a second reagent. The first and second reagents reservoirs are separated by a gaseous body in the channel. The sample and the first and second reagents are supplied to the detecting part due to an action of a pump part which is communicated to the first and second channels. Accordingly, it is possible to prevent a non-detection target nucleic monocle from being mixed and a nucleic acid sample from being leaked to the outside.

    摘要翻译： 核酸盒包括其中形成有第一和第二通道的主体。 含有靶核酸的样品通过注射口输入到第二通道的样品室中。 在盒中，包括检测部，其包括与第一和第二通道连通的检测室和核酸检测单元。 第一通道具有存储第一试剂储存器的第一试剂储存器和存储第二试剂的第二试剂储存器。 第一和第二试剂容器在通道中被气体体分开。 由于与第一和第二通道连通的泵部的动作，将样品和第一试剂和第二试剂供给到检测部。 因此，可以防止未检测到的靶核酸单核心体被混合，并且核酸样品泄漏到外部。