公开(公告)号：EP3059320A1

公开(公告)日：2016-08-24

申请号：EP14853421.7

申请日：2014-10-14

申请人： Kaneka Corporation

发明人： KANAYA, Kento ,  KAWAI, Yoshikazu ,  OKUBO, Takahiro ,  MORIO, Ken-ichiro

IPC分类号： C12P19/14 ,  B01J20/24 ,  B01J20/30 ,  C07K1/22 ,  C08B15/10 ,  G01N30/88

CPC分类号： C07K1/22 ,  B01D15/00 ,  B01J20/24 ,  B01J20/267 ,  B01J20/28016 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3085 ,  B01J20/3212 ,  B01J20/3274 ,  B01J2220/54 ,  B01J2220/82 ,  C02F1/286 ,  C08J9/00 ,  C08J9/26 ,  C08J2201/026 ,  C08J2201/046 ,  C08J2301/02 ,  C12P19/14 ,  G01N2030/525

摘要： The objective of the present invention is to provide to a method for easily producing high-performance porous cellulose beads having high mechanical strength. Also, the objective of the present invention is to provide an adsorbent produced from the high-performance porous cellulose beads. According to the present invention, high-performance porous cellulose beads can be easily produced from porous cellulose beads, and an adsorbent having high strength and high adsorption amount can be easily produced from the high-performance porous cellulose beads.

摘要翻译： 本发明的目的是提供一种用于容易地生产具有高机械强度的高性能多孔纤维素珠粒的方法。 此外，本发明的目的是提供由高性能多孔纤维素珠粒生产的吸附剂。 根据本发明，可以容易地从多孔纤维素珠制备高性能多孔纤维素珠，并且可以容易地从高性能多孔纤维素珠生产具有高强度和高吸附量的吸附剂。

公开(公告)号：EP2165758A1

公开(公告)日：2010-03-24

申请号：EP08764904.2

申请日：2008-05-30

申请人： Kaneka Corporation

发明人： KAWAI, Yoshikazu ,  KAWAHARA, Naomi

IPC分类号： B01J20/24 ,  A61M1/18 ,  C07K1/22 ,  C07K17/02 ,  G01N30/88

CPC分类号： C07K1/16 ,  B01D15/00 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3274 ,  C07K1/22

摘要： The present invention relates to a porous base matrix having formyl group, which base matrix has a structure represented by the formula (2) as a spacer, which structure is obtained by cleaving a group represented by the formula (1):


wherein, R 1 represents a group forming a five-membered ring or a six-membered ring with -CH (OH) -CH (OH) -; a method for producing the porous base matrix; and an adsorbent obtained by binding a ligand on the porous base matrix. By the present invention, a high-intensity base matrix for an adsorbent and an adsorbent are provided. The amount of a ligand leaked from the adsorbend is small.

摘要翻译： 本发明涉及一种多孔基底基质具有甲酰基，其中基本矩阵具有由式（2）作为间隔物，其中结构是由切割表示的基团由式（1）中得到表示的结构：worin，R 1 代表基团形成五元环或具有-CH（OH）-CH一个六元环（OH） - ; 用于生产多孔基矩阵的方法; 和在吸附剂上通过多孔基底基质结合的配体得到。 通过本发明，提供了用于在吸附剂和吸附剂，以高强度基矩阵。 从吸附物泄漏的配体的量是小的。

公开(公告)号：EP3050902B1

公开(公告)日：2018-11-21

申请号：EP14849556.7

申请日：2014-09-26

申请人： Kaneka Corporation

发明人： OKUBO, Takahiro ,  KAWAI, Yoshikazu ,  HIRANO, Masaru ,  KONOIKE, Fuminori ,  KARASUGI, Keiichi ,  HONDA, Tatsuya

IPC分类号： B01J20/24 ,  B01J20/28 ,  B01J20/26 ,  B01J20/285 ,  B01J20/286 ,  B01J20/30 ,  B01J20/32 ,  B01D15/08 ,  B01D15/38 ,  C07K1/22 ,  G01N30/88 ,  C08B15/00 ,  C08B15/08 ,  C08B15/10 ,  C08B16/00 ,  C08B37/12

CPC分类号： C07K1/22 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28016 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3071 ,  B01J20/3085 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3274 ,  B01J20/3293 ,  B01J2220/52 ,  C07K16/00 ,  C08B1/08 ,  C08B3/06 ,  C08B15/005 ,  C08B15/08 ,  C08B15/10 ,  C08B16/00 ,  C08J3/16 ,  C08J9/00 ,  C08J2301/02 ,  C08J2301/04 ,  C08J2301/12 ,  C08L1/02 ,  C08L1/04 ,  C08L1/12 ,  C08L2205/18

摘要： A process for producing porous cellulose beads of the present invention is characterized by comprising the steps of: a) mixing an alkali aqueous solution and cellulose to prepare cellulose micro dispersion at low temperature, b) adding water to the cellulose micro dispersion to prepare cellulose slurry, and d) bringing the cellulose slurry into contact with coagulation solvent. A carrier for ligand immobilization of the present invention is characterized by being by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate % = 1 ˆ’ V 2 / V 1 × 100 (wherein, V 1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V 2 indicates the gel volume of polysaccharide porous beads after shrinkage).

公开(公告)号：EP2626381A1

公开(公告)日：2013-08-14

申请号：EP11823691.8

申请日：2011-09-12

申请人： Kaneka Corporation

发明人： HIRANO, Masaru ,  SUZUKI, Yasuyuki ,  KANAYA, Kento ,  KANDA, Akihisa ,  SUZUKI, Takuma ,  KAWAI, Yoshikazu ,  MORIO, Ken-ichiro

IPC分类号： C08J9/28 ,  C08J3/16

CPC分类号： C08B1/003 ,  B01D15/3809 ,  B01J20/262 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/285 ,  B01J20/3212 ,  B01J20/3274 ,  C07K1/22 ,  C08J3/16 ,  C08J9/28 ,  C08J2201/0482 ,  C08J2207/12 ,  C08J2301/02 ,  Y10T428/2982

摘要： The objective of the present invention is to provide a method for easily producing a porous cellulose particle which can be used as an adsorbent for various substances in a safe manner without using a highly toxic solvent such as a calcium thiocyanate solution. In addition, the objective of the present invention is to provide a porous particle produced by the production method, an adsorbent which contains the porous particle and by which a highly pure protein can be efficiently purified in a safe manner, and a method for purifying a protein by using the adsorbent. The method for producing a porous particle according to the present invention is characterized in comprising the steps of preparing a solution containing cellulose and an ionic liquid; preparing a dispersion by dispersing the obtained cellulose solution into a liquid, wherein the liquid is not compatible with the ionic liquid; and coagulating the dispersion by bringing into contact with an alcohol or an alcohol aqueous solution in order in order to obtain the porous particle.

摘要翻译： 本发明的目的是提供一种容易地生产多孔纤维素颗粒的方法，其可以用于安全地用作各种物质的吸附剂，而不使用高毒性溶剂如硫氰酸钙溶液。 此外，本发明的目的是提供一种通过该制造方法制造的多孔质粒子，含有多孔质粒子的吸附剂，能够高效纯化高纯度蛋白质的方法， 蛋白质。 本发明的多孔质粒的制造方法的特征在于，包括以下工序：制造含有纤维素和离子性液体的溶液; 通过将获得的纤维素溶液分散到液体中制备分散体，其中液体与离子液体不相容; 并通过与醇或醇水溶液接触来依次凝固分散体，以获得多孔颗粒。

公开(公告)号：EP4386082A1

公开(公告)日：2024-06-19

申请号：EP22855868.0

申请日：2022-08-08

申请人： Kaneka Corporation ,  RIKEN

发明人： KAMBAYASHI, Sho ,  KAWAI, Yoshikazu ,  HAYASHI, Yohei ,  TAKASAKI, Mami

IPC分类号： C12N5/074 ,  C12N5/0735

CPC分类号： C12N5/06

摘要： The death of some pluripotent stem cells in suspension culture of pluripotent stem cells is avoided. When performing the suspension culture of the pluripotent stem cells, a liquid medium that does not substantially contain a PKCβ inhibitor is used for a single-cell state and a liquid medium containing a PKCβ inhibitor and a WNT inhibitor is used after a cell mass is formed.

公开(公告)号：EP4183867A1

公开(公告)日：2023-05-24

申请号：EP21843534.5

申请日：2021-07-13

申请人： Kaneka Corporation

发明人： KAWAI, Yoshikazu

IPC分类号： C12N5/071 ,  C12M3/00

摘要： A large amount of cardiomyocyte spheroids is obtained from dissociated cardiomyocytes in a simple manner, in a short time, without using a special culture medium component, and without using a complicatedly-shaped container or a minute container. The problems can be solved by a method for producing cardiomyocyte spheroids, the method including allowing dissociated cardiomyocytes to flow under suspension conditions in a non-annular container to aggregate the cells. In addition, the problems can also be solved by a method for producing cardiomyocyte spheroids, in which the dissociated cardiomyocytes have a history of stabilization culture after thawing.

公开(公告)号：EP3459631A1

公开(公告)日：2019-03-27

申请号：EP18199393.2

申请日：2014-09-26

申请人： Kaneka Corporation

发明人： OKUBO, Takahiro ,  KAWAI, Yoshikazu ,  HIRANO, Masaru ,  KONOIKE, Fuminori ,  KARASUGI, Keiichi ,  HONDA, Tatsuya

IPC分类号： B01J20/24 ,  B01J20/26 ,  B01J20/286 ,  B01J20/30 ,  B01J20/32 ,  B01D15/38 ,  C07K1/22

摘要： A process for producing porous cellulose beads of the present invention is characterized by comprising the steps of:
a) mixing an alkali aqueous solution and cellulose to prepare cellulose micro dispersion at low temperature,
b) adding water to the cellulose micro dispersion to prepare cellulose slurry, and
d) bringing the cellulose slurry into contact with coagulation solvent.
A carrier for ligand immobilization of the present invention is characterized by being by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate % = 1 − V 2 / V 1 × 100 (wherein, V 1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V 2 indicates the gel volume of polysaccharide porous beads after shrinkage).

公开(公告)号：EP3059252B1

公开(公告)日：2018-12-05

申请号：EP14853802.8

申请日：2014-10-14

申请人： Kaneka Corporation

发明人： HAYASHI, Asuka ,  KAWAI, Yoshikazu ,  MORIO, Ken-ichiro

IPC分类号： B01J20/24 ,  B01J20/285 ,  B01J20/286 ,  B01J20/30 ,  B01J20/32 ,  C07K1/22 ,  B01J2/06 ,  C08B1/10 ,  C08B15/10 ,  C08J9/28 ,  G01N30/88 ,  C07K1/14 ,  C07K17/12 ,  C08J9/16

CPC分类号： B01J20/24 ,  B01J20/285 ,  B01J20/286 ,  B01J20/305 ,  B01J20/3085 ,  B01J20/3212 ,  B01J20/3217 ,  B01J20/3274 ,  B01J20/3293 ,  C07K1/145 ,  C07K1/22 ,  C07K17/12 ,  C08B15/10 ,  C08J9/16 ,  C08J9/28 ,  C08J2201/026 ,  C08J2201/0547 ,  C08J2301/00

摘要： The objective of the present invention is to provide a method for easily and efficiently producing cellulose beads which have pore shape suitable for an adsorbent and of which adsorption performance is excellent without using highly toxic and highly corrosive auxiliary raw material and without industrially disadvantageous cumbersome step. The method for producing porous cellulose beads according to the present invention is characterized in comprising (a) the step of preparing a fine cellulose dispersion by mixing a low temperature alkaline aqueous solution and cellulose, (b) the step of preparing a mixed liquid by adding a crosslinking agent to the fine cellulose dispersion, (c) the step of preparing an emulsion by dispersing the mixed liquid in a dispersion medium, (d) the step of contacting the emulsion with a coagulating solvent.

公开(公告)号：EP3284778A1

公开(公告)日：2018-02-21

申请号：EP16780059.8

申请日：2016-04-13

申请人： Kaneka Corporation

发明人： KAWAI, Yoshikazu ,  HAYASHI, Asuka ,  HIRANO, Masaru ,  KAWAHATA, Yosuke

IPC分类号： C08J9/28 ,  B01D15/08 ,  B01J20/24 ,  B01J20/30 ,  G01N30/88

CPC分类号： B01J20/24 ,  B01D15/08 ,  B01D15/3809 ,  B01J20/28016 ,  B01J20/285 ,  B01J20/30 ,  B01J20/3085 ,  B01J2220/52 ,  C08J9/28 ,  G01N30/482 ,  G01N30/88

摘要： The objective of the present invention is to provide a method for easily and efficiently producing cellulose beads which have pore shape suitable for an adsorbent and of which adsorption performance is excellent without using highly toxic and highly corrosive auxiliary raw material and without an industrially disadvantageous cumbersome step. The method for producing porous cellulose beads according to the present invention is characterized in comprising (a) the step of mixing a low temperature alkaline aqueous solution and cellulose to prepare a fine cellulose dispersion, (b) the step of adding a crosslinking agent to the fine cellulose dispersion to prepare a mixed liquid, (c) the step of preparing an emulsion by dispersing the mixed liquid in a dispersion medium, and (d) the step of contacting the emulsion with a coagulating solvent.

摘要翻译： 本发明的目的在于提供一种不使用高毒性且高腐蚀性的辅助原料，且不存在工业上不利的麻烦的工序的，容易且高效地制造具有适合于吸附剂的孔形状且吸附性能优异的纤维素珠的方法 。 本发明的多孔性纤维素珠的制造方法的特征在于，包含：（a）将低温的碱性水溶液和纤维素混合而制备微细纤维素分散液的工序;（b）向所述 （c）将该混合液分散在分散介质中而制备乳液的工序;（d）使该乳液与凝固溶剂接触的工序。

公开(公告)号：EP2684897A1

公开(公告)日：2014-01-15

申请号：EP12754262.9

申请日：2012-03-06

申请人： Kaneka Corporation

发明人： KAWAI, Yoshikazu ,  SUZUKI, Yasuyuki ,  MORIO, Kenichiro ,  HIRANO, Masaru

IPC分类号： C08B1/08 ,  B01J2/06 ,  B01J20/24 ,  B01J20/30 ,  C08B15/10 ,  G01N30/88

CPC分类号： C08B15/10 ,  B01J20/267 ,  B01J20/28078 ,  B01J20/285 ,  B01J20/3085 ,  C08B15/00 ,  C08J9/28 ,  C08J2201/0546 ,  C08J2301/02 ,  C08L1/02

摘要： The objective of the present invention is to provide a convenient method for producing porous cellulose beads having high mechanical strength without using an auxiliary material which is highly toxic and corrosive and without a cumbersome and industrially adverse step. The method for producing porous cellulose beads according to the present invention is characterized in comprising the steps of mixing a cold alkaline aqueous solution and cellulose to prepare a cellulose dispersion and bringing the cellulose dispersion into contact with a coagulating solvent.

摘要翻译： 本发明的目的是提供一种方便的制造机械强度高的多孔纤维素珠粒的方法，而不使用高毒性和腐蚀性的辅助材料，而且不存在麻烦和工业上不利的步骤。 本发明的多孔性纤维素珠粒的制造方法的特征在于，包括将冷的碱性水溶液和纤维素混合而制备纤维素分散液，使该纤维素分散液与凝固溶剂接触的工序。