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1.发明公开
公开(公告)号：EP3327029A1
公开(公告)日：2018-05-30
申请号：EP16827817.4
申请日：2016-07-21
申请人： Kaneka Corporation
发明人： NISHIHACHIJYO, Masakatsu , NAKANO, Yoshiyuki , KONOIKE, Fuminori , TAKANO, Masayuki , YOSHIDA, Shinichi , MINAKUCHI, Kazunobu
IPC分类号： C07K14/195 , C07K1/22 , C07K17/00 , C07K19/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12P21/08
CPC分类号： C07K14/31 , C07K1/22 , C07K14/195 , C07K17/00 , C07K19/00 , C07K2319/30 , C12N5/10 , C12N15/09
摘要： The present invention provides a Protein A ligand that has a reduced antibody-binding capacity in an acidic pH range when the ligand is immobilized on a carrier to prepare an affinity separation matrix. The present invention provides a protein containing an amino acid sequence derived from any of the E, D, A, B, and C domains of Protein A of SEQ ID NOs: 1 to 5 in which a hydrophobic amino acid residue in the Fc binding site is substituted by a different hydrophobic amino acid residue or polar uncharged amino acid residue, wherein the protein has a reduced antibody-binding capacity in an acidic pH range as compared to before the substitution.
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2.发明公开
公开(公告)号：EP3459631A1
公开(公告)日：2019-03-27
申请号：EP18199393.2
申请日：2014-09-26
申请人： Kaneka Corporation
发明人： OKUBO, Takahiro , KAWAI, Yoshikazu , HIRANO, Masaru , KONOIKE, Fuminori , KARASUGI, Keiichi , HONDA, Tatsuya
摘要： A process for producing porous cellulose beads of the present invention is characterized by comprising the steps of:
a) mixing an alkali aqueous solution and cellulose to prepare cellulose micro dispersion at low temperature,
b) adding water to the cellulose micro dispersion to prepare cellulose slurry, and
d) bringing the cellulose slurry into contact with coagulation solvent.
A carrier for ligand immobilization of the present invention is characterized by being by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate % = 1 − V 2 / V 1 × 100 (wherein, V 1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V 2 indicates the gel volume of polysaccharide porous beads after shrinkage).-
3.发明公开METHOD FOR PRODUCING POROUS CELLULOSE BEADS USING ALKALI AQUEOUS SOLUTION, CARRIER FOR LIGAND IMMOBILIZATION, AND ADSORBENT 审中-公开用于生产多孔纤维素珠使用碱水溶液，载体配体和吸附的固定化
公开(公告)号：EP3050902A1
公开(公告)日：2016-08-03
申请号：EP14849556.7
申请日：2014-09-26
申请人： Kaneka Corporation
发明人： OKUBO, Takahiro , KAWAI, Yoshikazu , HIRANO, Masaru , KONOIKE, Fuminori , KARASUGI, Keiichi , HONDA, Tatsuya
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/24 , B01J20/267 , B01J20/28004 , B01J20/28016 , B01J20/285 , B01J20/286 , B01J20/3071 , B01J20/3085 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , C07K16/00 , C08B1/08 , C08B3/06 , C08B15/005 , C08B15/08 , C08B15/10 , C08B16/00 , C08J3/16 , C08J9/00 , C08J2301/02 , C08J2301/04 , C08J2301/12 , C08L1/02 , C08L1/04 , C08L1/12 , C08L2205/18
摘要： A process for producing porous cellulose beads of the present invention is characterized by comprising the steps of:
a) mixing an alkali aqueous solution and cellulose to prepare cellulose micro dispersion at low temperature,
b) adding water to the cellulose micro dispersion to prepare cellulose slurry, and
d) bringing the cellulose slurry into contact with coagulation solvent.
A carrier for ligand immobilization of the present invention is characterized by being by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate % = 1 − V 2 / V 1 × 100 (wherein, V 1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V 2 indicates the gel volume of polysaccharide porous beads after shrinkage).摘要翻译： 一种用于制造本发明的多孔纤维素珠的方法是通过包括以下步骤为特征：a）碱性wässrige溶液和纤维素的混合，以在低温下制备纤维素微分散体，b）添加水至纤维素微分散，以制备纤维素浆料 ，以及d）使所述纤维素浆料与凝固溶剂接触。 收缩率（％）= 1“V 2 /：用于本发明的配体固定化载体是通过由下式定义的收缩率收缩多糖多孔珠粒不小于10％是，和交联多糖多孔珠粒为特征的 V 1×100（worin，V 1表示收缩前的多糖多孔珠粒凝胶体积，和V 2表示收缩后的的多糖多孔珠粒凝胶体积）。
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公开(公告)号：EP2894171A1
公开(公告)日：2015-07-15
申请号：EP13834932.9
申请日：2013-09-09
申请人： Kaneka Corporation
发明人： KAWAI, Yoshikazu , MORIO, Ken-ichiro , KONOIKE, Fuminori , WATANABE, Kana , SUZUKI, Yasuyuki , HIRANO, Masaru
CPC分类号： A61M1/3679 , A61K9/1652 , C08B15/10 , C08B16/00 , C08J3/126 , C08J3/16 , C08J2301/02 , C08J2405/02 , C08J2489/00 , C08L1/02 , C08L5/02 , C08L89/00
摘要： The objective of the present invention is to obtain an adsorbent having high adsorption capacity and high strength comprising porous cellulose beads obtained without using an auxiliary material which is highly toxic and corrosive and without a cumbersome and industrially adverse step. The present invention is characterized by immobilizing a ligand onto porous cellulose beads obtained by mixing a cold alkaline aqueous solution and cellulose powder as a raw material to prepare a cellulose dispersion and bringing the cellulose dispersion into contact with a coagulating solvent.
摘要翻译： 本发明的目的是获得具有高吸附能力和高强度的吸附剂，其包含不使用高毒性和腐蚀性且无笨重和工业上不利的步骤的辅助材料而获得的多孔纤维素珠粒。 本发明的特征在于将配体固定在通过混合冷碱水溶液和纤维素粉末作为原料而获得的多孔纤维素珠粒上，以制备纤维素分散体并使纤维素分散体与凝固溶剂接触。
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公开(公告)号：EP4206329A1
公开(公告)日：2023-07-05
申请号：EP21861155.6
申请日：2021-08-03
申请人： KANEKA CORPORATION
摘要： The objective of the present invention is to provide a method for purifying a useful substance with effectively suppressing the occurrence of precipitate after an impurity is adsorbed to be removed on the specific adsorbent from a useful substance-containing liquid. The method for purifying a useful substance from a useful substance-containing liquid according to the first present invention is characterized in comprising the steps of contacting the useful substance-containing liquid with a hardly-soluble magnesium compound, removing the hardly-soluble magnesium compound from the useful substance-containing liquid contacted with the hardly-soluble magnesium compound to obtain a treated liquid, and adjusting pH of the treated liquid to 8.0 or less. The method for purifying a useful substance from a useful substance-containing liquid according to the second present invention is characterized in comprising the steps of contacting the useful substance-containing liquid with a hardly-soluble magnesium compound, and contacting the useful substance-containing liquid with an aluminum compound.
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公开(公告)号：EP3845548A1
公开(公告)日：2021-07-07
申请号：EP19854100.5
申请日：2019-08-23
申请人： KANEKA CORPORATION
摘要： The objective of the present invention is to provide a method capable of efficiently removing an impurity from an aqueous solution or a suspension comprising an antibody or an antibody-like molecule and the impurity. The method for purifying an antibody or an antibody-like molecule according to the present invention is characterized in treating an aqueous solution or a suspension comprising the antibody or the antibody-like molecule and an impurity with a water-insoluble inorganic compound, wherein the water-insoluble inorganic compound comprises one or more elements selected from magnesium, calcium and aluminum.
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公开(公告)号：EP3050902B1
公开(公告)日：2018-11-21
申请号：EP14849556.7
申请日：2014-09-26
申请人： Kaneka Corporation
发明人： OKUBO, Takahiro , KAWAI, Yoshikazu , HIRANO, Masaru , KONOIKE, Fuminori , KARASUGI, Keiichi , HONDA, Tatsuya
IPC分类号： B01J20/24 , B01J20/28 , B01J20/26 , B01J20/285 , B01J20/286 , B01J20/30 , B01J20/32 , B01D15/08 , B01D15/38 , C07K1/22 , G01N30/88 , C08B15/00 , C08B15/08 , C08B15/10 , C08B16/00 , C08B37/12
CPC分类号： C07K1/22 , B01D15/3809 , B01J20/24 , B01J20/267 , B01J20/28004 , B01J20/28016 , B01J20/285 , B01J20/286 , B01J20/3071 , B01J20/3085 , B01J20/3212 , B01J20/3219 , B01J20/3274 , B01J20/3293 , B01J2220/52 , C07K16/00 , C08B1/08 , C08B3/06 , C08B15/005 , C08B15/08 , C08B15/10 , C08B16/00 , C08J3/16 , C08J9/00 , C08J2301/02 , C08J2301/04 , C08J2301/12 , C08L1/02 , C08L1/04 , C08L1/12 , C08L2205/18
摘要： A process for producing porous cellulose beads of the present invention is characterized by comprising the steps of: a) mixing an alkali aqueous solution and cellulose to prepare cellulose micro dispersion at low temperature, b) adding water to the cellulose micro dispersion to prepare cellulose slurry, and d) bringing the cellulose slurry into contact with coagulation solvent. A carrier for ligand immobilization of the present invention is characterized by being by shrinking polysaccharide porous beads not less than 10% by a shrinkage rate defined by the following formula, and crosslinking the polysaccharide porous beads: Shrinkage rate % = 1 ˆ’ V 2 / V 1 × 100 (wherein, V 1 indicates the gel volume of polysaccharide porous beads before shrinkage, and V 2 indicates the gel volume of polysaccharide porous beads after shrinkage).
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