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    MUTANT POLYHYDROXYALKANOIC ACID SYNTHASE, GENE AND TRANSFORMANT THEREOF, AND METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID
    3.
    发明公开
    MUTANT POLYHYDROXYALKANOIC ACID SYNTHASE, GENE AND TRANSFORMANT THEREOF, AND METHOD FOR PRODUCING POLYHYDROXYALKANOIC ACID 审中-公开

    公开(公告)号：EP3741858A1

    公开(公告)日：2020-11-25

    申请号：EP19740819.8

    申请日：2019-01-10

    申请人： Kaneka Corporation

    发明人： KOBAYASHI, Shingo ,  YOSHIDA, Shinichi ,  SATO, Shunsuke ,  TAOKA, Naoaki

    IPC分类号： C12N15/60 ,  C12N1/21 ,  C12N9/88 ,  C12P7/62

    摘要： Provided is a mutant PHA synthetase which enables production of a PHA copolymer with a high or low 3HH ratio while maintaining PHA productivity. The mutant PHA synthetase is a mutant polyhydroxyalkanoate synthetase having an amino acid sequence having 85% or more sequence identity with the amino acid sequence of SEQ ID NO: 1 and comprising any one or more of the following mutations (a) to (c): mutation (a): mutation of substitution of serine at 389th position from N-terminus of the amino acid sequence of SEQ ID NO: 1 with an amino acid other than serine; mutation (b): mutation of substitution of leucine at 436th position from the N-terminus of the amino acid sequence of SEQ ID NO: 1 with an amino acid other than leucine; and mutation (c): mutation of deletion of 11 or more and 19 or less amino acid residues from C-terminus of the amino acid sequence of SEQ ID NO: 1.

    MICROORGANISM HAVING MULTIPLE GENES ENCODING PHA SYNTHASE AND METHOD FOR PRODUCING PHA USING SAME
    6.
    发明公开
    MICROORGANISM HAVING MULTIPLE GENES ENCODING PHA SYNTHASE AND METHOD FOR PRODUCING PHA USING SAME 审中-公开
    具有多菌对PHA合成酶基因编码和这样就产生PHA方法

    公开(公告)号：EP3124615A1

    公开(公告)日：2017-02-01

    申请号：EP15769649.3

    申请日：2015-03-27

    申请人： Kaneka Corporation

    发明人： KOBAYASHI, Shingo ,  AOKI, Rina ,  MINAMI, Tetsuya ,  ARIKAWA, Hisashi ,  MATSUMOTO, Keiji

    IPC分类号： C12P7/62 ,  C08G63/06 ,  C12N1/21 ,  C08L101/16 ,  C12N15/09

    CPC分类号： C12P7/625 ,  C08G63/06 ,  C08L101/16 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/09 ,  C12Y203/01 ,  C12Y402/01017

    摘要： A PHA copolymer which is slowly crystallized is improved in crystallization speed to improve the melt workability of the PHA copolymer in working such as injection molding, film molding, blow molding, fiber spinning, extrusion foaming or bead foaming, thereby improving the resultant articles in productivity. A method for the improvement is a method for producing a PHA mixture, including the step of culturing a microorganism having both of a gene encoding a PHA synthase that synthesizes a copolymer PHA (A) and that is derived from the genus Aeromonas, and a gene encoding a PHA synthase that synthesizes a PHA (B) different in melting point from the copolymer PHA (A) by 10°C or more to produce, in a cell of the microorganism, two or more PHAs different in melting point from one another by 10°C or more simultaneously.

    摘要翻译： 甲PHA共聚物的所有其缓慢结晶在结晶化速度提高，以改善工作的PHA共聚物的熔体可加工性：诸如注射成型，薄膜成型，吹塑成型，纤维纺丝，挤出发泡或珠粒发泡，从而提高生产率所得制品 ， 一种用于改进的方法是用于生产PHA混合物，其中包括培养具有两种基因的编码PHA合酶做合成的共聚物的PHA（A）的微生物的步骤的方法，也被从气单胞菌属，和一个基因的 编码PHA合酶没来合成PHA（B）在从共聚物PHA（A）熔点相差10℃以上，以产生，在所述微生物中，两个或更多的PHA在熔点由彼此不同的小区 10℃以上的同时。