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    5-SUBSTITUTED HYDANTOIN RACEMASE, DNA ENCODING THE SAME, RECOMBINANT DNA, TRANSFORMED CELL, AND PROCESS FOR PRODUCTION OF OPTICALLY ACTIVE N-CARBAMYLAMINO ACID OR OPTICALLY ACTIVE AMINO ACID
    2.
    发明公开
    5-SUBSTITUTED HYDANTOIN RACEMASE, DNA ENCODING THE SAME, RECOMBINANT DNA, TRANSFORMED CELL, AND PROCESS FOR PRODUCTION OF OPTICALLY ACTIVE N-CARBAMYLAMINO ACID OR OPTICALLY ACTIVE AMINO ACID 审中-公开
    5-取代的乙内酰脲消旋酶编码DNA，重组DNA，转化细胞及其制造方法光学活性N-CARBAMYLAMINOSÄUREOR光学活性氨基

    公开(公告)号：EP1852510A1

    公开(公告)日：2007-11-07

    申请号：EP06712435.4

    申请日：2006-01-26

    申请人： Kaneka Corporation

    发明人： NISHI, Kenichi ,  YANAGISAWA, Satohiro ,  NANBA, Hirokazu ,  UEDA, Makoto ,  NORO, Naoto

    IPC分类号： C12N15/61 ,  C12N1/21 ,  C12N9/90 ,  C12P17/10 ,  C12P41/00

    CPC分类号： C12P13/04 ,  C12N9/90 ,  C12P41/009

    摘要： Disclosed are a novel hydantoin racemase and a process for producing an optically active N-carbamylamino acid or an optically active amino acid using the hydantoin racemase. A novel hydantoin racemase isolated and purified from Bacillus sp. Strain KNK519HR; a gene encoding the hydantoin racemase; a recombinant plasmid having the gene introduced therein; a transformant having the hydantoin racemase gene introduced therein; and a process for producing an optically active N-carbamylamino acid or an optically active amino acid characterized in that a 5-substituted hydantoin compound is treated in the presence of hydantoinase and N-carbamylamino acid amidohydolase as well as the hydantoin racemase.

    摘要翻译： 本发明公开了一种新颖的乙内酰脲消旋酶和用于在光学活性N- carbamylamino酸或使用乙内酰脲消旋光学活性的氨基酸的生产的方法。 一种新颖的乙内酰脲消旋酶分离和来自芽孢杆菌属的纯化。 应变KNK519HR; 编码乙内酰脲消旋酶的基因; 具有该基因的重组质粒引入其中; 具有乙内酰脲消旋酶基因的转化体引入其中; 并在光学活性N- carbamylamino酸或其特征在于光学活性的氨基酸的生产过程做了5 substituiertem乙内酰脲化合物在乙内酰脲和N- carbamylamino酸的存在下处理amidohydolase以及乙内酰脲消旋酶。