公开(公告)号：EP4310178A1

公开(公告)日：2024-01-24

申请号：EP22771538.0

申请日：2022-03-18

申请人： Keio University

发明人： ISHII, Hiroko ,  OKANO, Hideyuki ,  KONDO, Takahiro ,  SATO, Yuta

IPC分类号： C12N5/079 ,  C12N15/12 ,  C12N15/864 ,  C12Q1/06 ,  G01N33/15

摘要： A method for producing a brain organoid having an aggregated tau protein including a step (a) of culturing pluripotent stem cells in the presence of a SMAD inhibitor to form an embryoid body, a step (b) of embedding the embryoid body in an extracellular matrix and three-dimensionally culturing the embryoid body in the presence of a SMAD inhibitor and a GSK3β inhibitor to form an organoid that includes neural precursor cells, a step (c) of extracting the organoid from the extracellular matrix and suspension-culturing the organoid in the presence of LIF to form a brain organoid, a step (d) of forcing the brain organoid to express a mutant MAPT gene, and a step (e) of further suspension-culturing the brain organoid after step (d) to obtain a brain organoid having an aggregated tau protein.