公开(公告)号：EP1465984A1

公开(公告)日：2004-10-13

申请号：EP03704437.7

申请日：2003-01-16

申请人： Kobenhavns Universitet

发明人： PETERSEN, Lone, Ronnov-Jessen ,  PETERSEN, Ole, William ,  GODJONSSON, Thorarinn ,  VILLADSEN, René ,  BISSELL, Mona, J.

IPC分类号： C12N5/08 ,  A61K35/12 ,  A61K48/00 ,  G01N33/50

CPC分类号： C12N5/0631 ,  A61K35/12 ,  C12N2503/02 ,  C12N2510/00 ,  C12N2510/04 ,  G01N33/5011 ,  G01N33/5017 ,  G01N2333/4742

摘要： A method for isolating cells which have a suprabasal position and expresses epithelial specific antigen but no sialomucin is provided. The isolated cells are shown to share many of the properties expected of a mammary gland stem cell. Three permanent cell lines that are capable of proliferating and capable of differentiating into cells of mammary gland luminal epithelial and myoepithelial cell lineages were established. Such cells form elaborate branching structures resembling uncultured terminal duct lobular units both by morphology and marker expression both in vitro and in vivo. Evidence is provided that these keratin K19 expressing cells most probably is the cells in which breast cancer arises. Thus they constitute a model not only for the developing breast, but also for the development of breast cancer. Also disclosed are the uses of such isolated cells or the cell lines as a model system of the mammary gland for pharmacological studies and their uses in tissue repair or transplantation.

摘要翻译： 提供了一种分离具有上基础位置并表达上皮特异性抗原但不含唾液酸粘蛋白的细胞的方法。 分离的细胞被证明具有乳腺干细胞预期的许多性质。 建立了能够增殖并能分化成乳腺腔上皮细胞和肌上皮细胞谱系细胞的三种永久性细胞系。 这样的细胞通过体外和体内的形态学和标志物表达形成类似于未培养的末端管小叶单位的精细分枝结构。 证据表明这些角蛋白K19表达细胞最可能是乳腺癌发生的细胞。 因此，它们不仅是发展中的乳房，而且也是乳腺癌发展的模型。 还公开了这种分离的细胞或细胞系作为乳腺的模型系统用于药理学研究及其在组织修复或移植中的用途的用途。

公开(公告)号：EP1414954A2

公开(公告)日：2004-05-06

申请号：EP02758178.4

申请日：2002-07-09

申请人： Kobenhavns Universitet

发明人： PETERSEN, Ole, William ,  NIELSEN, Helga, Lind ,  PETERSEN, Lone, Ronnov-Jessen

IPC分类号： C12N9/24 ,  C12N9/00 ,  C12N15/11 ,  C12N15/12 ,  C12N15/62 ,  C12Q1/68 ,  C07K14/47 ,  C07K16/18 ,  A61K38/43 ,  A61K38/17

CPC分类号： C07K14/47 ,  A61K35/12 ,  A61K38/00 ,  A61K2039/505 ,  A61K2039/5156 ,  C07K2319/00 ,  C12N9/2402

摘要： The present invention describes a novel gene, EPSTI1 for epithelial stromal interaction 1 (breast), which is upregulated upon direct interaction between tumour cells and stromal cells in the tumour environment of the breast. The full-length EPSTI1 cDNA was isolated and characterized with respect to nucleotide sequence, chromosomal organization and localization, i.e. the nucleotide sequence encoding the EPSTI1 polypeptide, the EPSTI1 polypeptide itself is disclosed herein. Furthermore, the present invention discloses the use of said gene for production of pure EPSTI1-protein. Finally the use of the EPSTI1 gene as a tool or diagnosis and prognosis of cancer, especially as a specific toll to detect metastatic cancer and invasive cancer is disclosed.

摘要翻译： 本发明描述了一种在直接相互作用肿瘤细胞和基质细胞之间在乳腺肿瘤环境中上调的新基因，EPSTI1上皮基质相互作用1（乳腺），全部。 该全长cDNA EPSTI1什么分离的和相对于核苷酸序列，染色体定位和组织，即特征的 编码多肽EPSTI1，所述EPSTI1多肽本身在盘游离缺失的核苷酸序列。 进一步地，本发明盘松动生产纯EPSTI1蛋白的使用所述基因的。 最后使用EPSTI1基因作为工具或诊断和预后癌肿，爱特别是作为一个特定的通行费，以检测转移性癌症和浸润性癌是游离缺失盘。