公开(公告)号：EP3281987A1

公开(公告)日：2018-02-14

申请号：EP17184830.2

申请日：2017-08-04

申请人： Konica Minolta, Inc.

发明人： Ishii, Hironori ,  Aimiya, Takuji ,  Takanashi, Kensaku ,  Nakayama, Shin

IPC分类号： C09B67/08 ,  C08F2/44 ,  C09B67/02 ,  G01N33/53 ,  G01N33/58

CPC分类号： G01N33/582 ,  C08F2/22 ,  C08F2/44 ,  C08J7/047 ,  C08J2325/14 ,  C08J2461/24 ,  C08J2461/26 ,  C08J2461/28 ,  C09B67/0005 ,  C09B67/0013 ,  C09B67/0097 ,  G01N33/53 ,  G01N33/54346 ,  G01N33/587 ,  C08F212/08 ,  C08F220/44 ,  C08F220/20 ,  C08F2220/287

摘要： The present invention provides a method of producing core/shell-type fluorescent dye-containing nanoparticles for immunohistochemical staining or live cell imaging, the method including: the step 1 of polymerizing monomers for thermoplastic resin synthesis in the presence of a fluorescent dye and thereby preparing core particles composed of a thermoplastic resin containing the fluorescent dye; and the step 2 of coating the core particles each with a shell layer composed of a thermosetting resin. By the method of producing fluorescent dye-containing nanoparticles according to the present invention, fluorescent dye-containing nanoparticles having a high brightness, whose dye does not elutes into water, physiological saline, culture medium and the like, can be produced, and the fluorescent dye-containing nanoparticles can be effectively utilized in immunohistochemical staining and live cell imaging.

摘要翻译： 本发明提供一种制备用于免疫组织化学染色或活细胞成像的包含核/壳型荧光染料的纳米颗粒的方法，所述方法包括：步骤1：在荧光染料存在下聚合用于热塑性树脂合成的单体，由此制备 由含有荧光染料的热塑性树脂构成的芯颗粒; 以及将核心颗粒各自涂覆由热固性树脂构成的壳层的步骤2。 通过本发明的含有荧光色素的纳米粒子的制造方法，可以制造染料不会溶出到水中的高亮度的含有荧光色素的纳米粒子，生理盐水，培养基等， 含染料的纳米粒子可以有效地用于免疫组织化学染色和活细胞成像。