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    A METHOD FOR CONTROLLING THE MAIN COMPLEX N-GLYCAN STRUCTURES AND THE ACIDIC VARIANTS AND VARIABILITY IN BIOPROCESSES PRODUCING RECOMBINANT PROTEINS
    2.
    发明公开
    A METHOD FOR CONTROLLING THE MAIN COMPLEX N-GLYCAN STRUCTURES AND THE ACIDIC VARIANTS AND VARIABILITY IN BIOPROCESSES PRODUCING RECOMBINANT PROTEINS 审中-公开
    方法用于控制主复合N-聚糖结构和酸奶变异与变异性生物过程产生重组蛋白

    公开(公告)号：EP2697251A1

    公开(公告)日：2014-02-19

    申请号：EP12713737.0

    申请日：2012-04-12

    申请人： LEK Pharmaceuticals d.d.

    发明人： KONCILJA, Matja ,  SPUDIC, Vatroslav ,  STOJKOVIC, Sasa ,  TISU, Matja

    IPC分类号： C07K14/435 ,  C12N5/00 ,  C07K14/43

    CPC分类号： C07K16/2887 ,  C07K16/22 ,  C07K16/241 ,  C07K16/2863 ,  C12N1/38 ,  C12P21/02

    摘要： The present invention relates to a method of controlling quality and quantity of posttranslational modification of a recombinantly produced polypeptide/protein (glycoprotein), wherein the posttranslational modification affects the glycosylation profile and/or the acidic variants profile, as manifested in CEX profiles, wherein the polypeptide/protein (glycoprotein) production is in eukaryotic host cells, the method comprising the following steps: a) cultivating the eukaryotic cells in a suitable medium under conditions which allow the expression of the polypeptide/protein, wherein the content of the dissolved CO 2 (pCO 2 ) in the medium is at a first value during the initial growth phase of the eukaryotic cells, allowing the eukaryotic cells to grow, and b) increasing or decreasing the content of the dissolved CO 2 (pCO 2 ) in the medium during the production phase of the eukaryotic cells to a second value.

    摘要翻译： 本发明涉及一种控制重组产生的多肽/蛋白质（糖蛋白）的翻译后修饰的质量和数量，worin翻译后修饰的方法会影响糖基化特征和/或所述酸性变体轮廓，如在CEX轮廓表现出来，worin的 多肽/蛋白质（糖蛋白）的生产是在真核宿主细胞，所述方法包括以下步骤：a）培养在合适的培养基中的真核细胞的条件，其允许所述多肽/蛋白质的表达下，worin溶解的含量的CO 2 （PCO 2）在介质中是在过程中的真核细胞的初始生长阶段的第一值，允许所述真核细胞生长，和b）增加或期间在培养基中降低的溶解的CO 2（二氧化碳分压）的含量 所述真核细胞的生产阶段到第二值。