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    Process for controlling contamination of nucleic acid amplification reactions
    1.
    发明公开
    Process for controlling contamination of nucleic acid amplification reactions 失效
    Verfahren zur Beherrschung der Verunreinigung vonNukleinsäure-Amplifizierungsreaktionen。

    公开(公告)号：EP0522884A1

    公开(公告)日：1993-01-13

    申请号：EP92306396.0

    申请日：1992-07-13

    申请人： LIFE TECHNOLOGIES INC.

    发明人： Hartley, James L. ,  Berninger, Mark

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6848 ,  C12Q2531/137 ,  C12Q2525/101 ,  C12Q2521/531 ,  C12Q2525/121 ,  C12Q2521/319

    摘要： A method of incorporating an exo-sample nucleotide (e.g. deoxyuridine in DNA) into amplified product strands resulting from a nucleic acid amplification process is disclosed. Once the product strands have been obtained and analysed (e.g. by hybridisation, Southern blot, etc.), the exo-sample strands can be selectively destroyed by acting on the incorporated exo-sample nucleotide.
    Two procedures are described. In the first procedure the exo-sample nucleotide is incorporated by conducting the amplification reaction in the presence of an excess of exo-sample nucleotide triphosphate.
    In the second procedure the exo-sample nucleotide is incorporated by conducting the amplification reaction in the presence of an oligonucleotide which has, as part of its sequence, one or more exo-sample nucleotides.

    摘要翻译： 公开了将外切样品核苷酸（例如DNA中脱氧尿苷）掺入由核酸扩增过程产生的扩增产物链中的方法。 一旦获得并分析了产物链（例如通过杂交，Southern印迹等），可以通过作用于合并的外切核苷酸来选择性地破坏外部样品链。 描述两个步骤。 在第一个步骤中，通过在过量的外切核苷酸三磷酸的存在下进行扩增反应来引入外切核苷酸。 在第二程序中，通过在寡核苷酸的存在下进行扩增反应来引入外部样品核苷酸，所述寡核苷酸作为其序列的一部分，具有一个或多个外切核苷酸。

    Amplification of nucleic acid sequences using random sequence oligonucleotides as primers
    2.
    发明公开
    Amplification of nucleic acid sequences using random sequence oligonucleotides as primers 失效
    使用随机序列的寡核苷酸作为引物的核酸序列的扩增。

    公开(公告)号：EP0395398A2

    公开(公告)日：1990-10-31

    申请号：EP90304496.4

    申请日：1990-04-26

    申请人： LIFE TECHNOLOGIES INC.

    发明人： Hartley, James L.

    IPC分类号： C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/708 ,  C12Q1/6844 ,  C12Q1/6858 ,  Y10S435/81 ,  Y10T436/143333 ,  C12Q2537/143 ,  C12Q2525/179

    摘要： A process for substantially amplifying template nucleic acid present in a sample is described, wherein the amplification may be performed without the need for prior knowledge of specific sequences. The process comprises apposition of random oligonucleotide primers to the template nucleic acid under conditions such that extension products of the primers are synthesised which are complementary to the template nucleic acid.

    摘要翻译： 一种用于基本上扩增模板样品中存在的核酸方法描述worin扩增可能而不需要特定序列的现有知识来执行。 该过程检查并的引物延伸产物合成了与模板核酸互补的条件下包括随机寡核苷酸引物与模板核酸并置。

    Process for controlling contamination of nucleic acid amplification reactions
    5.
    发明公开
    Process for controlling contamination of nucleic acid amplification reactions 失效
    控制核酸放大反应污染的方法

    公开(公告)号：EP0401037A3

    公开(公告)日：1991-09-18

    申请号：EP90306001.0

    申请日：1990-06-01

    申请人： LIFE TECHNOLOGIES INC.

    发明人： Hartley, James L.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/708 ,  C12Q1/6848 ,  C12Q2531/113 ,  C12Q2525/101

    摘要： An amplification procedure is performed on a first sample in which one or more of the four normal ribonucleoside triphosphates(rNTPs) or deoxyribonucleoside triphosphates (dNTPs) is replaced with an exo-sample nucleotide that is not present in nucleic acid to be amplified. After amplification, any contaminating amplified product that may be remaining is subjected to a physical, chemical, enzymatic, or biological treatment which renders nucleic acid containing the exo-sample nucleotide substantially unamplifiable. The treatment may be done as a separate step or it may be done in the presence of a second sample containing nucleic acid sequences to be amplified. The amplified nucleic acid sequences derived from the first sample which contaminate the second sample are not further substantially amplified during amplification of nucleic acid sequences of the second sample.

    Process for controlling contamination of nucleic acid amplification reactions
    8.
    发明公开
    Process for controlling contamination of nucleic acid amplification reactions 失效
    Verfahren zur Beherrschung von Kontamination derNukleinsäure-Amplifikation。

    公开(公告)号：EP0401037A2

    公开(公告)日：1990-12-05

    申请号：EP90306001.0

    申请日：1990-06-01

    申请人： LIFE TECHNOLOGIES INC.

    发明人： Hartley, James L.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/708 ,  C12Q1/6848 ,  C12Q2531/113 ,  C12Q2525/101

    摘要： An amplification procedure is performed on a first sample in which one or more of the four normal ribonucleoside triphosphates(rNTPs) or deoxyribonucleoside triphosphates (dNTPs) is replaced with an exo-sample nucleotide that is not present in nucleic acid to be amplified. After amplification, any contaminating amplified product that may be remaining is subjected to a physical, chemical, enzymatic, or biological treatment which renders nucleic acid containing the exo-sample nucleotide substantially unamplifiable. The treatment may be done as a separate step or it may be done in the presence of a second sample containing nucleic acid sequences to be amplified. The amplified nucleic acid sequences derived from the first sample which contaminate the second sample are not further substantially amplified during amplification of nucleic acid sequences of the second sample.

    摘要翻译： 对其中四种正常核糖核苷三磷酸（rNTP）或脱氧核糖核苷三磷酸（dNTP）中的一种或多种被不存在于待扩增的核酸中的外切核苷酸替代的第一样品进行扩增程序。 扩增后，可能残留的任何污染的扩增产物进行物理，化学，酶学或生物处理，使含有外切核苷酸的核酸基本上不可扩增。 治疗可以作为单独的步骤进行，或者可以在含有待扩增的核酸序列的第二个样品的存在下进行。 衍生自污染第二样品的第一样品的扩增的核酸序列在第二样品的核酸序列的扩增期间不被进一步基本上扩增。