公开(公告)号：EP1637037A3

公开(公告)日：2010-06-02

申请号：EP05026614.7

申请日：1993-02-12

申请人： LIFECELL CORPORATION

发明人： Livesey, Stephen A. ,  Del Campo, Anthony A. ,  Nag, Abhijit ,  Nichols, Ken B. ,  Griffey, Edward S. ,  Coleman, Christopher

IPC分类号： A61L27/36 ,  A01N1/00

CPC分类号： A61L27/3625 ,  A01N1/00 ,  A01N1/02 ,  A01N1/0226 ,  A61K35/18 ,  A61K39/12 ,  A61K39/13 ,  A61L27/3604 ,  A61L27/362 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3695 ,  A61L2430/40 ,  C12N1/04 ,  C12N2770/32634 ,  F25C1/142 ,  F26B5/065

摘要： A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage and rehydration under conditions that preclude functionally significant damage and reconstitution with viable cells.

公开(公告)号：EP0475409B1

公开(公告)日：1998-04-15

申请号：EP91115480.5

申请日：1991-09-12

申请人： LIFECELL CORPORATION

发明人： Livesey, Stephen ,  Del Campo, Anthony A. ,  Nag, Abhijit ,  Nichols, Ken B. ,  Piunno, Carmen ,  Ross, David

IPC分类号： A01N1/02 ,  F26B5/06

CPC分类号： A01N1/0221 ,  A01N1/02 ,  A01N1/0257 ,  A61K35/18 ,  A61K39/12 ,  A61K39/13 ,  A61L2430/40 ,  C12N1/04 ,  C12N2770/32634 ,  F25C1/142 ,  F26B5/065

摘要： This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in a appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.

公开(公告)号：EP0475409A3

公开(公告)日：1993-09-01

申请号：EP91115480.5

申请日：1991-09-12

申请人： LIFECELL CORPORATION

发明人： Livesey, Stephen ,  Del Campo, Anthony A. ,  Nag, Abhijit ,  Nichols, Ken B. ,  Piunno, Carmen ,  Ross, David

IPC分类号： A01N1/02 ,  F26B5/06

CPC分类号： A01N1/0221 ,  A01N1/02 ,  A01N1/0257 ,  A61K35/18 ,  A61K39/12 ,  A61K39/13 ,  A61L2430/40 ,  C12N1/04 ,  C12N2770/32634 ,  F25C1/142 ,  F26B5/065

摘要： This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in a appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.

公开(公告)号：EP1637037A2

公开(公告)日：2006-03-22

申请号：EP05026614.7

申请日：1993-02-12

申请人： LIFECELL CORPORATION

发明人： Livesey, Stephen A. ,  Del Campo, Anthony A. ,  Nag, Abhijit ,  Nichols, Ken B. ,  Griffey, Edward S. ,  Coleman, Christopher

IPC分类号： A01N1/00 ,  A61L27/36

CPC分类号： A61L27/3625 ,  A01N1/00 ,  A01N1/02 ,  A01N1/0226 ,  A61K35/18 ,  A61K39/12 ,  A61K39/13 ,  A61L27/3604 ,  A61L27/362 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3695 ,  A61L2430/40 ,  C12N1/04 ,  C12N2770/32634 ,  F25C1/142 ,  F26B5/065

摘要： A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage and rehydration under conditions that preclude functionally significant damage and reconstitution with viable cells.

摘要翻译： 一种用于处理和用于移植无细胞的基于胶原的组织基质的保存方法游离缺失盘。 该方法包括：处理生物组织中的稳定溶液降低采购损坏的步骤，与处理液处理以除去细胞，治疗与随后的条件下冷冻，干燥，储存和再水合冷冻保护剂溶液没有排除功能显著损伤和重构 用活细胞。

公开(公告)号：EP0475409A2

公开(公告)日：1992-03-18

申请号：EP91115480.5

申请日：1991-09-12

申请人： LIFECELL CORPORATION

发明人： Livesey, Stephen ,  Del Campo, Anthony A. ,  Nag, Abhijit ,  Nichols, Ken B. ,  Piunno, Carmen ,  Ross, David

IPC分类号： A01N1/02 ,  F26B5/06

CPC分类号： A01N1/0221 ,  A01N1/02 ,  A01N1/0257 ,  A61K35/18 ,  A61K39/12 ,  A61K39/13 ,  A61L2430/40 ,  C12N1/04 ,  C12N2770/32634 ,  F25C1/142 ,  F26B5/065

摘要： This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in a appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.

摘要翻译： 本发明涉及的方法，装置和解决方案的时间生物学过长冷冻保存微观生物材料。 该方法包括处理中的悬浮液的生物材料，在适当的缓冲液，与冷冻保护剂的冷冻保护剂或组合，其提高了样品的玻璃化转变温度范围。 一种或多种干燥保护剂可被添加到所述cryosolution。 然后将cryosolution被雾化并与新的装置快速冷却，通过分子蒸馏干燥，储存，然后在其使用之前的缓冲器再水化。 这些解决方案包括冷冻保护剂的新混合物。

公开(公告)号：EP0564786B1

公开(公告)日：2005-12-07

申请号：EP93102264.4

申请日：1993-02-12

申请人： LIFECELL CORPORATION

发明人： Livesey, Stephen A. ,  Del Campo, Anthony A. ,  Nag, Abhijit ,  Nichols, Ken B. ,  Griffey, Edward S. ,  Coleman, Christopher

IPC分类号： A01N1/02 ,  A61L27/00

CPC分类号： A61L27/3625 ,  A01N1/00 ,  A01N1/02 ,  A01N1/0226 ,  A61K35/18 ,  A61K39/12 ,  A61K39/13 ,  A61L27/3604 ,  A61L27/362 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3695 ,  A61L2430/40 ,  C12N1/04 ,  C12N2770/32634 ,  F25C1/142 ,  F26B5/065

摘要： A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage and rehydration under conditions that preclude functionally significant damage and reconstitution with viable cells.

公开(公告)号：EP0564786A3

公开(公告)日：1994-07-06

申请号：EP93102264.4

申请日：1993-02-12

申请人： LIFECELL CORPORATION

发明人： Livesey, Stephen A. ,  Del Campo, Anthony A. ,  Nag, Abhijit ,  Nichols, Ken B. ,  Griffey, Edward S. ,  Coleman, Christopher

IPC分类号： A61L27/00 ,  A01N1/00

CPC分类号： A61L27/3625 ,  A01N1/00 ,  A01N1/02 ,  A01N1/0226 ,  A61K35/18 ,  A61K39/12 ,  A61K39/13 ,  A61L27/3604 ,  A61L27/362 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3695 ,  A61L2430/40 ,  C12N1/04 ,  C12N2770/32634 ,  F25C1/142 ,  F26B5/065

摘要： A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage and rehydration under conditions that preclude functionally significant damage and reconstitution with viable cells.

公开(公告)号：EP0564786A2

公开(公告)日：1993-10-13

申请号：EP93102264.4

申请日：1993-02-12

申请人： LIFECELL CORPORATION

发明人： Livesey, Stephen A. ,  Del Campo, Anthony A. ,  Nag, Abhijit ,  Nichols, Ken B. ,  Griffey, Edward S. ,  Coleman, Christopher

IPC分类号： A61L27/00 ,  A01N1/00

CPC分类号： A61L27/3625 ,  A01N1/00 ,  A01N1/02 ,  A01N1/0226 ,  A61K35/18 ,  A61K39/12 ,  A61K39/13 ,  A61L27/3604 ,  A61L27/362 ,  A61L27/3683 ,  A61L27/3687 ,  A61L27/3695 ,  A61L2430/40 ,  C12N1/04 ,  C12N2770/32634 ,  F25C1/142 ,  F26B5/065

摘要： A method for processing and preserving an acellular collagen-based tissue matrix for transplantation is disclosed. The method includes the steps of processing biological tissues with a stabilizing solution to reduce procurement damage, treatment with a processing solution to remove cells, treatment with a cryoprotectant solution followed by freezing, drying, storage and rehydration under conditions that preclude functionally significant damage and reconstitution with viable cells.

摘要翻译： 一种用于处理和用于移植无细胞的基于胶原的组织基质的保存方法游离缺失盘。 该方法包括：处理生物组织中的稳定溶液降低采购损坏的步骤，与处理液处理以除去细胞，治疗与随后的条件下冷冻，干燥，储存和再水合冷冻保护剂溶液没有排除功能显著损伤和重构 用活细胞。