公开(公告)号：EP1666606A4

公开(公告)日：2006-11-29

申请号：EP04772285

申请日：2004-08-20

申请人： LOCOMOGENE INC ,  EISAI CO LTD

发明人： NAKAJIMA TOSHIHIRO ,  AMANO TETSUYA ,  ZHANG LEI ,  IKEDA RIE ,  YAMASAKI SATOSHI ,  YAGISHITA NAOKO ,  YOKOHAMA HIROMITSU ,  KOGUSHI MOTOJI

IPC分类号： C12Q1/25 ,  A61K38/00 ,  A61K38/16 ,  A61P29/00 ,  C12Q1/26

CPC分类号： A61K38/16 ,  A61K38/005 ,  C12Q1/25 ,  C12Q1/26 ,  G01N2333/90245 ,  G01N2500/00

摘要： It is intended to provide a method for evaluating an effect of controlling the activity of proline 4-hydroxyolase; a screening method with the use of the above evaluation method; and a method of screening a compound which is useful in treating or preventing fibrosis or rheumatoid arthritis. A method for detecting an effect of controlling the ubiquitination activity of proline 4-hydroxyolase alpha subunit (P4HA1) by synoviolin; and a method of screening a substance having an effect of controlling the ubiquitination activity of P4HA1 of synoviolin based on the above method. A compound found out by this screening is useful in treating or preventing diseases caused by disorders in the activity of proline 4-hydroxylase such as fibrosis and rheumatoid arthritis.

公开(公告)号：EP1352961A4

公开(公告)日：2004-06-16

申请号：EP01272293

申请日：2001-12-21

申请人： LOCOMOGENE INC

发明人： NAKAJIMA TOSHIHIRO ,  AMANO TETSUYA

IPC分类号： A01K67/027 ,  A61K38/00 ,  C07K14/47 ,  C07K14/705 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N15/12 ,  C12N15/85 ,  G01N33/564 ,  G01N33/574 ,  C12N15/52 ,  A61K31/713 ,  A61K38/53 ,  C07K16/40 ,  C12N5/10 ,  C12N9/00 ,  C12N15/63 ,  C12N15/90 ,  C12Q1/68 ,  G01N33/50

CPC分类号： C12N15/8509 ,  A01K67/0275 ,  A01K67/0276 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2217/072 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/0325 ,  A61K38/00 ,  C12N9/104 ,  C12N9/93 ,  C12Y203/02 ,  C12Y603/02 ,  G01N33/564 ,  G01N33/574 ,  G01N2500/00

摘要： The present invention discloses a novel protein called Synoviolin and a gene that encodes it. This protein is expressed specifically by synovial tissue and also accompanies the presence of an auto-antibody that recognizes this protein in rheumatoid arthritis (RA) patients. The protein according to the present invention and its antibody can be expected to be used as specific diagnostic markers for RA. In addition, the gene or protein according to the present invention may be used to permit the screening of drugs to treat RA. Moreover, the present invention provides synoviolin gene transgenic animals. The transgenic animals according to the present invention can be used as RA model animals in the development of pharmaceuticals to treat RA.

摘要翻译： 公开了一种新的蛋白质滑膜增生因子和编码它的基因。 该蛋白质在滑膜组织中特异性表达，并且与识别类风湿性关节炎（RA）患者中的该蛋白质的自身抗体相关联。 预计该蛋白质或其抗体可用作RA特异性诊断标记物。 此外，预计通过使用上述基因或蛋白质可以筛选RA治疗。 还提供了滑膜增生因子基因的转基因动物。 该转基因动物可用作开发RA治疗药物的RA模型动物。

公开(公告)号：EP1780221A4

公开(公告)日：2007-10-03

申请号：EP05758020

申请日：2005-07-04

申请人： LOCOMOGENE INC

发明人： NAKAJIMA TOSHIHIRO ,  YAMASAKI SATOSHI ,  ZHANG LEI ,  AMANO TETSUYA

IPC分类号： C07K16/40 ,  A61K39/395 ,  A61P19/02 ,  A61P29/00 ,  A61P35/00 ,  C12N5/10 ,  C12N9/99 ,  C12N15/02 ,  C12P21/08 ,  C12Q1/02 ,  G01N33/53

CPC分类号： C12Q1/25 ,  A61K2039/505 ,  C07K16/40 ,  C07K2317/76

摘要： An antibody, or fragment thereof, against synoviolin for providing a monoclonal antibody capable of recognizing a part of synoviolin, which antibody against synoviolin is capable of inhibiting the self-ubiquitinization of synoviolin.

摘要翻译： 抗滑膜生长因子的抗体或其片段用于提供能够识别滑膜增生因子的一部分的单克隆抗体，该滑膜生长因子抗体能够抑制滑膜增生因子的自我遍在蛋白化。

公开(公告)号：EP1669452A4

公开(公告)日：2007-06-27

申请号：EP04772380

申请日：2004-08-23

申请人： LOCOMOGENE INC

发明人： NAKAJIMA TOSHIHIRO ,  AMANO TETSUYA ,  TSUCHIMOCHI KANEYUKI ,  YAMASAKI SATOSHI ,  YAGISHITA NAOKO

IPC分类号： C12N15/12 ,  A61K35/76 ,  A61K48/00 ,  A61P29/00 ,  C07K14/47 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10

CPC分类号： C07K14/47

摘要： Promoter of synoviolin gene comprising at least a fragment of 2120th to 2130th nucleotides of the base sequence of SEQ ID NO. 1 or 2 or comprising at least a fragment of 1st to 2201st, 969th to 2201st, 1142nd to 2201st, 1699th to 2201st, 1880th to 2201st, 2002nd to 2201st, 2094th to 2201st or 2118th to 2201st nucleotides of the base sequence of SEQ ID NO. 1 or 2; and a method of regulating the transcriptive activity characterized in that the activity of this promoter is inhibited or enhanced.

公开(公告)号：EP1367123A4

公开(公告)日：2005-10-12

申请号：EP02710489

申请日：2002-02-04

申请人： LOCOMOGENE INC

发明人： NAKAJIMA TOSHIHIRO

IPC分类号： A01K67/027 ,  A61K39/00 ,  A61K45/00 ,  A61M1/34 ,  A61M1/36 ,  A61P25/02 ,  C07K14/47 ,  C07K16/18 ,  C07K19/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/00 ,  C12N15/09 ,  C12N15/12 ,  C12P21/08 ,  C12Q1/04 ,  G01N33/15 ,  G01N33/50 ,  G01N33/53 ,  G01N33/566 ,  G01N33/577 ,  G01N33/68

CPC分类号： G01N33/6896 ,  A01K2217/05 ,  C07K14/47 ,  C07K16/18 ,  G01N2500/00

摘要： It is intended to specify a substance causative of hyperexcitability in Isaacs syndrome to thereby establish efficacious diagnostic and therapeutic methods therefor. A specific cDNA is isolated from a nerve cell-origin cDNA library by an immunoscreening technique with the use of the serum of a patient with Isaacs syndrome. The nucleotide sequence of the cDNA is clarified and a novel protein neurotonin is obtained by cloning based on the same. Then the amino acid sequence structure of neurotonin is determined. This novel protein, which is considered as the key factor in Isaacs syndrome, is useful in studies on peripheral nerve disorders and clinical application.