公开(公告)号：EP3504342A1

公开(公告)日：2019-07-03

申请号：EP17761768.5

申请日：2017-08-25

申请人： Life Technologies Corporation

发明人： PAGANI, Ioanna ,  ZERINGER, Emily ,  LI, Kelly ,  HUANG, Boli

IPC分类号： C12Q1/68 ,  C12Q1/70

公开(公告)号：EP3472349A1

公开(公告)日：2019-04-24

申请号：EP17733322.6

申请日：2017-06-16

申请人： Life Technologies Corporation

发明人： PAGANI, Ioanna ,  BRZOSKA, Pius ,  LI, Kelly ,  PATEL, Sunali ,  HUANG, Boli ,  VARMA, Kamini ,  PURI, Nitin ,  DIAMOND, Evan

IPC分类号： C12Q1/68

公开(公告)号：EP2279194A1

公开(公告)日：2011-02-02

申请号：EP09739272.4

申请日：2009-01-16

申请人： Life Technologies

发明人： MENCHEN, Steven, M. ,  ROSENBLUM, Barnett ,  KENNEY, Paul ,  KHAN, Shoeb, I. ,  MA, Zhaochun ,  CHEN, Jer-kang ,  LAM, Joseph ,  HUANG, Boli

IPC分类号： C07H19/073 ,  C07H21/04 ,  C12Q1/68 ,  C07H19/173

CPC分类号： C07H21/04 ,  C07H19/073 ,  C07H19/173 ,  C12Q1/6874 ,  C12Q2523/107 ,  C12Q2525/186 ,  C12Q2525/197

摘要： Nucleotide analogs that can sustain the enzymatic synthesis of double-stranded nucleic acid from a nucleic template are described. The nucleotide analogs include: (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine, uracil and their analogs; (ii) a label attached to the base or analog of the base via a cleavable linker; (iii) a deoxyribose; and (iv) one or more phosphate groups. The linker and/or the label inhibits template directed polymerase incorporation of a further nucleotide substrate onto an extended primer strand. In addition, cleavage of the linker leaves a residue attached to the base which is not present in the natural nucleotide and which does not inhibit extension of the primer strand. The nucleotide analogs can therefore be used as reversible terminators in sequencing by synthesis methods without blocking the 3' hydroxyl group. Methods of sequencing DNA using the substrates are also described.