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公开(公告)号：EP3327440A1
公开(公告)日：2018-05-30
申请号：EP17191391.6
申请日：2013-02-13
发明人： VLASSOV, Alexander , LI, Mu , ZERINGER, Emily , CONRAD, Richard
摘要： Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
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公开(公告)号：EP3327440B1
公开(公告)日：2020-01-08
申请号：EP17191391.6
申请日：2013-02-13
发明人： VLASSOV, Alexander , LI, Mu , ZERINGER, Emily , CONRAD, Richard
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公开(公告)号：EP3504342A1
公开(公告)日：2019-07-03
申请号：EP17761768.5
申请日：2017-08-25
发明人： PAGANI, Ioanna , ZERINGER, Emily , LI, Kelly , HUANG, Boli
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4.发明授权ALKYLENE GLYCOLS AND POLYMERS AND COPOLYMERS THEREOF FOR DIRECT ISOLATION OF NUCLEIC ACID FROM EMBEDDED SAMPLES 有权二醇和聚合物和共聚物及其用于从包埋的样品直接分离核酸
公开(公告)号：EP2646551B1
公开(公告)日：2017-06-07
申请号：EP11797080.6
申请日：2011-11-30
CPC分类号： C12N15/1003 , C12Q1/6804 , C12Q2527/125 , C12Q2565/518
摘要： Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mn of 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffmization step prior to isol- ating nucleic acids from paraffm-embedded samples, for example.
摘要翻译： 直接提供分离核酸从包埋的生物样品的方法。 乳化的摘要是在热塑性表蛋白酶的存在而产生，并且添加剂从亚烷基二醇，聚（亚烷基二醇），或具有在76平均M n 2900的嵌段共聚物，或者其盐或衍生物或它们的组合中选择 ， 核酸从乳化消化直接分离。 方法消除了使用有机溶剂：例如在隔离石蜡包埋的样品核酸，例如之前的脱石蜡工序二甲苯。
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公开(公告)号：EP2839278B1
公开(公告)日：2017-09-27
申请号：EP13706853.2
申请日：2013-02-13
发明人： VLASSOV, Alexander , LI, Mu , ZERINGER, Emily , CONRAD, Richard
IPC分类号： G01N33/50
CPC分类号： G01N1/34 , C12Q1/6806 , G01N33/5005 , G01N33/5076 , G01N33/92
摘要： Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
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公开(公告)号：EP2839278A1
公开(公告)日：2015-02-25
申请号：EP13706853.2
申请日：2013-02-13
发明人： VLASSOV, Alexander , LI, Mu , ZERINGER, Emily , CONRAD, Richard
IPC分类号： G01N33/50
CPC分类号： G01N1/34 , C12Q1/6806 , G01N33/5005 , G01N33/5076 , G01N33/92
摘要： Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.
摘要翻译： 公开了用于从生物流体和组织分离外来体的方法，组合物和试剂盒。 使用不含体积聚合物来从生物样品中沉淀外来体，从而通过低速（台式）离心或过滤允许外来体分离。 还描述了沉淀后进一步分离外泌体。
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7.发明公开ALKYLENE GLYCOLS AND POLYMERS AND COPOLYMERS THEREOF FOR DIRECT ISOLATION OF NUCLEIC ACID FROM EMBEDDED SAMPLES 有权二醇和聚合物和共聚物及其用于从包埋的样品直接分离核酸
公开(公告)号：EP2646551A1
公开(公告)日：2013-10-09
申请号：EP11797080.6
申请日：2011-11-30
CPC分类号： C12N15/1003 , C12Q1/6804 , C12Q2527/125 , C12Q2565/518
摘要： Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mn of 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffmization step prior to isol- ating nucleic acids from paraffm-embedded samples, for example.
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公开(公告)号：EP1654361B1
公开(公告)日：2012-04-18
申请号：EP04820001.8
申请日：2004-07-26
IPC分类号： C12N15/10
CPC分类号： C12Q1/6806 , C12N15/1003 , C12N15/1006 , C12N15/1096 , C12Q1/686
摘要： The present invention provides improved methods and compositions for RNA isolation. In particular embodiments the present invention concerns the use of methods and compositions for the isolation of full-length RNA from fixed tissue samples. The present invention provides methods for digesting and extracting RNA from a fixed tissue sample.
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