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公开(公告)号：EP3317426A1
公开(公告)日：2018-05-09
申请号：EP16741745.0
申请日：2016-07-01
发明人： FONNUM, Geir , MENCHEN, Steven M. , LUI, Alfred , XIANG, Guobing , LARSEN, Synne
IPC分类号： C12Q1/68 , G01N33/543
CPC分类号： C12Q1/6874 , C08J7/14 , C08J2333/26 , C12Q1/6853 , C12Q1/6869 , C12Q1/6876 , C12Q2525/197 , C12Q2527/125 , C12Q2565/607 , C12Q2565/629
摘要： A polymer substrate, such as a polymer coating or a polymer hydrogel network, includes carboxyl moieties that can be used as conjugation sites to which receptor or analyte molecules can be attached. In an example, the polymer substrate includes a polyacrylamide polymer network having alkanoic acid moieties or derivatives thereof, which can react with carboxyl activating compounds to provide an activated alkanoate moieties on the polyacrylamide network Amine-terminated nucleic acids can react with the activated alkanoate moieties to capture the nucleic acid to the polymer network through an alkylamide moiety.
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公开(公告)号：EP3317314B1
公开(公告)日：2020-01-08
申请号：EP16741201.4
申请日：2016-07-01
发明人： FONNUM, Geir , MENCHEN, Steven, M. , GOKMEN, Talha, M. , LUNDBERG, Pontus , THWAR, Prasanna Krishnan , LUI, Alfred , LU, Lily , HINZ, Wolfgang , HUSABOE, Lene , BREIVOLD, Elisabeth , MOLTEBERG, Astrid, Evenroed , LARSEN, Synne
IPC分类号： C08F220/58 , C08F8/12
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公开(公告)号：EP3702383A1
公开(公告)日：2020-09-02
申请号：EP20150423.0
申请日：2016-07-01
发明人： FONNUM, Geir , MENCHEN, Steven M. , GOKMEN, Talha M. , LUNDBERG, Pontus , THWAR, Prasanna Krishnan , LUI, Alfred , LU, Lily , HINZ, Wolfgang , HUSABOE, Lene , BREIVOLD, Elisabeth , MOLTEBERG, Astrid Evenroed , LARSEN, Synne
IPC分类号： C08F220/58 , C08F8/12
摘要： A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.
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公开(公告)号：EP3317426B1
公开(公告)日：2020-01-15
申请号：EP16741745.0
申请日：2016-07-01
发明人： FONNUM, Geir , MENCHEN, Steven M. , LUI, Alfred , XIANG, Guobing , LARSEN, Synne
IPC分类号： C12Q1/68 , G01N33/543
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公开(公告)号：EP3317314A1
公开(公告)日：2018-05-09
申请号：EP16741201.4
申请日：2016-07-01
发明人： FONNUM, Geir , MENCHEN, Steven, M. , GOKMEN, Talha, M. , LUNDBERG, Pontus , THWAR, Prasanna Krishnan , LUI, Alfred , LU, Lily , HINZ, Wolfgang , HUSABOE, Lene , BREIVOLD, Elisabeth , MOLTEBERG, Astrid, Evenroed , LARSEN, Synne
IPC分类号： C08F220/58 , C08F8/12
摘要： A polymer substrate, such as a polymer particle, is formed from a carboxyl functional monomer. In an example, the carboxyl functional monomer has a protection group in place of the OH of the carboxyl group. Once the monomer is polymerized, such a protection group can be removed, providing a polymer network with carboxyl functional sites. Such sites can be used to attach other functionality to the polymer substrate.
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公开(公告)号：EP3320115B1
公开(公告)日：2020-09-02
申请号：EP16742101.5
申请日：2016-07-05
发明人： MENCHEN, Steven M. , XIANG, Guobing , THWAR, Prasanna Krishnan , FREDIANI, Jeffrey , WONG, Allen , LUI, Alfred , LU, Lily
IPC分类号： C12Q1/6834 , C12Q1/6874
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公开(公告)号：EP3626831A1
公开(公告)日：2020-03-25
申请号：EP19190463.0
申请日：2013-02-08
发明人： MENCHEN, Steven , BLANCHARD, Alan , ANDRUZZI, Luisa , KHAN, Shaheer , GREMYACHINSKIY, Dmitriy , LUI, Alfred , STOLARCZYK, Craig , SOKOLSKY, Tanya
IPC分类号： C12Q1/68
摘要： A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.
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公开(公告)号：EP3228715A2
公开(公告)日：2017-10-11
申请号：EP17160771.6
申请日：2013-02-08
发明人： MENCHEN, Steven , BLANCHARD, Alan , ANDRUZZI, Luisa , KHAN, Shaheer , GREMYACHINSKIY, Dmitriy , LUI, Alfred , STOLARCZYK, Craig , SOKOLSKY, Tanya
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C08F8/40 , C12Q1/6834 , C12Q2523/101 , C12Q2563/149 , C12Q2565/119
摘要： A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.
摘要翻译： 缀合的衬底的方法包括：交换与亲脂性抗衡离子的生物分子相关的抗衡离子，以形成生物分子复合物，在非水溶剂中的生物分子复合物分散，并在非水溶剂的存在下偶联，所述生物分子复合物到基底上 。 底物或固体支持物是聚合物颗粒，要固定在颗粒上的生物分子是寡核苷酸，亲脂性抗衡离子是四丁基铵离子，非水溶剂是N-甲基吡咯烷酮（NMP）。 寡核苷酸包被的颗粒用于核酸分离或核酸测序反应。
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公开(公告)号：EP2812446B1
公开(公告)日：2017-05-10
申请号：EP13705357.5
申请日：2013-02-08
发明人： MENCHEN, Steven , BLANCHARD, Alan , ANDRUZZI, Luisa , KHAN, Shaheer , GREMYACHINSKIY, Dmitriy , LUI, Alfred , STOLARCZYK, Craig , SOKOLSKY, Tanya
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C08F8/40 , C12Q1/6834 , C12Q2523/101 , C12Q2563/149 , C12Q2565/119
摘要： A method of conjugating a substrate includes exchanging a counter ion associated with a biomolecule with a lipophilic counter ion to form a biomolecule complex, dispersing the biomolecule complex in a nonaqueous solvent, and coupling the biomolecule complex to a substrate in the presence of the nonaqueous solvent. The substrate or solid support is a polymeric particle, the biomolecule to be immobilized on the particle is an oligonucleotide, the lipophilic counter ion is a tetrabutylammonium ion and the nonaqueous solvent is N- methylpyrrolidone (NMP). The oligonucleotide coated particles are used in nucleic acid isolation or in nucleic acid sequencing reactions.
摘要翻译： 缀合基质的方法包括将与生物分子相关的抗衡离子与亲脂性反离子交换以形成生物分子复合物，将生物分子复合物分散在非水溶剂中，并在非水溶剂存在下将生物分子复合物偶联到底物 。
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公开(公告)号：EP3320115A1
公开(公告)日：2018-05-16
申请号：EP16742101.5
申请日：2016-07-05
发明人： MENCHEN, Steven M. , XIANG, Guobing , THWAR, Prasanna Krishnan , FREDIANI, Jeffrey , WONG, Allen , LUI, Alfred , LU, Lily
IPC分类号： C12Q1/68
摘要： A hydrogel network includes a hydrogel polymer having a coupling site, an oligonucleotide conjugated at a terminal end to the hydrogel polymer at the coupling site, and a functional moiety coupled between the terminal end of the oligonucleotide and the coupling site. Such a hydrogel network can be formed by a method including activating a coupling site of a substrate and binding a linker moiety coupled to a terminal end of an oligonucleotide to the activated coupling site, a functional moiety coupled between the terminal end of the oligonucleotide and the linker moiety.
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