公开(公告)号：EP1476549B1

公开(公告)日：2008-06-04

申请号：EP03703010.3

申请日：2003-02-25

申请人： Lifeind EHF.

发明人： JONSSON, Jon Johannes ,  THORMAR, Hans Guttormur ,  GUNNARSSON, Gudmundur H. ,  GUDMUNDSSON, Bjarki

IPC分类号： C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/101

摘要： Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that linear nucleic acid fragments of equal length but having different conformation are separated; said first and second electrophoresis conditions are different, such that in one of said dimensions electrophoresis allows separation of linear nucleic acid fragments based on conformation and length, and in the other of said dimensions electrophoresis allows separation of the sample fragments based substantially on length. Said difference is preferably established with a chemical agent capable of reducing conformational differences between linear nucleic acids fragments.

公开(公告)号：EP1791955B1

公开(公告)日：2011-02-16

申请号：EP05781069.9

申请日：2005-09-02

申请人： Lifeind EHF.

发明人： GUNNARSSON, Gudmundur H. ,  THORMAR, Hans Guttormur ,  GUDMUNDSSON, Bjarki ,  JONSSON, Jon Johannes

IPC分类号： C12N15/10 ,  G01N27/447 ,  G06F19/00

CPC分类号： C12N15/101 ,  C12N15/10 ,  G01N27/44726 ,  G01N27/44747 ,  G01N27/44773

摘要： A method is provided for separating single- and double-stranded nucleic acid molecules based on their strandness and length. The method is based on novel two-dimensional gel electrophoresis techniques comprises loading a sample of nucleic acid molecules in a gel electrophoresis apparatus and electrophoresing in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that populations of single- and double-stranded nucleic acids are separated, said first and second electrophoresis conditions being different, such that in one dimension electrophoresis allows separation of the sample molecules based on strandness and length, and in the other dimension electrophoresis allows separation based substantially on length, wherein said difference is established with a chemical agent and/or physical parameter affecting the strandness-dependent electrophoresis migration rate of nucleic acids

公开(公告)号：EP1791955A2

公开(公告)日：2007-06-06

申请号：EP05781069.9

申请日：2005-09-02

申请人： Lifeind EHF.

发明人： GUNNARSSON, Gudmundur H. ,  THORMAR, Hans Guttormur ,  GUDMUNDSSON, Bjarki ,  JONSSON, Jon Johannes

IPC分类号： C12N15/10 ,  G01N27/447 ,  G06F19/00

CPC分类号： C12N15/101 ,  C12N15/10 ,  G01N27/44726 ,  G01N27/44747 ,  G01N27/44773

摘要： A method is provided for separating single- and double-stranded nucleic acid molecules based on their strandness and length. The method is based on novel two-dimensional gel electrophoresis techniques comprises loading a sample of nucleic acid molecules in a gel electrophoresis apparatus and electrophoresing in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that populations of single- and double-stranded nucleic acids are separated, said first and second electrophoresis conditions being different, such that in one dimension electrophoresis allows separation of the sample molecules based on strandness and length, and in the other dimension electrophoresis allows separation based substantially on length, wherein said difference is established with a chemical agent and/or physical parameter affecting the strandness-dependent electrophoresis migration rate of nucleic acids

公开(公告)号：EP1476549A1

公开(公告)日：2004-11-17

申请号：EP03703010.3

申请日：2003-02-25

申请人： Lifeind EHF.

发明人： JONSSON, Jon Johannes ,  THORMAR, Hans Guttormur ,  GUNNARSSON, Gudmundur H. ,  GUDMUNDSSON, Bjarki

IPC分类号： C12N15/10 ,  C12Q1/68

CPC分类号： C12N15/101

摘要： Methods are provided for separating linear nucleic acid fragments based on their conformation. The methods are based on novel two-dimensional gel electrophoresis techniques. In one aspect the method comprises electrophoresing a sample of nucleic acids in a first dimension said sample through a gel matrix under a first set of pre-determined electrophoresis conditions; electrophoresing said gel matrix in a second dimension under a second set of electrophoresis conditions, such that linear nucleic acid fragments of equal length but having different conformation are separated; said first and second electrophoresis conditions are different, such that in one of said dimensions electrophoresis allows separation of linear nucleic acid fragments based on conformation and length, and in the other of said dimensions electrophoresis allows separation of the sample fragments based substantially on length. Said difference is preferably established with a chemical agent capable of reducing conformational differences between linear nucleic acids fragments.