公开(公告)号：EP2917731A4

公开(公告)日：2016-06-01

申请号：EP13854034

申请日：2013-11-08

申请人： MACFARLANE BURNET INST FOR MEDICAL RES & PUBLIC HEALTH LTD

发明人： ANDERSON DAVID ANDREW ,  GARCIA MARY LOUISE ,  BARNES NADINE CARMEL ,  HANAFIAH KHAYRIYYAH MOHD ,  LANDAY ALAN LEE

IPC分类号： G01N33/53 ,  C12N15/12

CPC分类号： C07K14/70503 ,  C07K2319/00 ,  G01N33/56983 ,  G01N33/6854 ,  G01N2333/08 ,  G01N2333/10 ,  G01N2333/16 ,  G01N2333/70535 ,  G01N2469/20 ,  G01N2800/06

摘要： The specification describes an antibody capture process comprising (i) obtaining a biological sample comprising antibodies, (ii) contacting the biological sample with recombinant pIgR or a dIgA-binding variant, wherein the pIgR or variant binds dIgA and forms a pIgR-dIgA complex. The process may further comprise (iii) directly or indirectly assessing the level of the pIgR-dIgA complex or the level of a complex between pIgR-dIgA and an antigen of interest. There is also an antibody capture process for determining gut wall integrity in a test subject, wherein the level or ratio of SIgA to dIgA is compared to a corresponding level or ratio from a control subject. The specification provides kits embodying the process and recombinant pIgR when used for, or for use, in capturing or detecting dIgA and/or IgM.

摘要翻译： 本说明书描述了抗体捕获方法，其包括（i）获得包含抗体的生物样品，（ii）使生物样品与重组pIgR或dIgA结合变体接触，其中pIgR或变体结合dIgA并形成pIgR-dIgA复合物。 该方法可进一步包括（iii）直接或间接评估pIgR-dIgA复合物的水平或pIgR-dIgA与感兴趣的抗原之间的复合物的水平。 还有一种用于确定测试对象中的肠壁完整性的抗体捕获方法，其中将SIgA与dIgA的水平或比例与来自对照受试者的相应水平或比例进行比较。 本说明书提供了用于捕获或检测dIgA和/或IgM或用于捕获或检测dIgA和/或IgM的方法和实施该方法的重组pIgR的试剂盒。