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1.发明授权INHIBITING TRANSFORMATION OF CELLS HAVING ELEVATED PURINE METABOLIC ENZYME ACTIVITY 失效抑制细胞转化具有增强的嘌呤代谢酶的活性
公开(公告)号：EP0458901B1
公开(公告)日：1997-12-17
申请号：EP90904105.5
申请日：1990-02-14
申请人： MASSACHUSETTS INSTITUTE OF TECHNOLOGY , HARVARD UNIVERSITY , THE GENERAL HOSPITAL CORPORATION , AMIRA, INC.
发明人： KADDURAH-DAOUK, Rima , DAOUK, Ghaleb , SCHIMMEL, Paul, R. , KINGSTON, Robert , LILLIE, James, W. , GREEN, Michael , PUTNEY, Scott, D.
CPC分类号： C12N15/1137 , A61K31/00 , A61K31/195 , A61K31/415 , A61K31/505 , A61K31/66 , A61K38/00 , A61K48/00 , C07K14/82 , C12N9/12 , C12N9/1223 , C12Q1/50 , C12Y207/0102 , C12Y207/03002 , C12Y207/04003 , C12Y406/01001 , G01N33/573 , G01N33/57484 , G01N2333/01
摘要： A method of inhibiting growth rate, transformation or metastasis of mammalian cells, particularly epithelial cells, in which activity of at least one enzyme which participates in purine metabolism and regulation of nucleotide levels is elevated. In particular, a method of inhibiting transformation of mammalian cells by a DNA tumor virus, a DNA tumor virus factor or other factor which has an equivalent effect on cells.
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2.发明公开INHIBITING TRANSFORMATION OF CELLS HAVING ELEVATED PURINE METABOLIC ENZYME ACTIVITY 失效抑制细胞转化具有增强的嘌呤代谢酶的活性。
公开(公告)号：EP0458901A1
公开(公告)日：1991-12-04
申请号：EP90904105.0
申请日：1990-02-14
发明人： KADDURAH-DAOUK, Rima , DAOUK, Ghaleb , SCHIMMEL, Paul, R. , KINGSTON, Robert , LILLIE, James, W. , GREEN, Michael , PUTNEY, Scott, D.
IPC分类号： A61K38 , A61K31 , A61K45 , A61K48 , A61P31 , A61P35 , C07D233 , C07D303 , C07H19 , C07K14 , C12N9 , C12N15 , C12Q1 , G01N33
CPC分类号： C12N15/1137 , A61K31/00 , A61K31/195 , A61K31/415 , A61K31/505 , A61K31/66 , A61K38/00 , A61K48/00 , C07K14/82 , C12N9/12 , C12N9/1223 , C12Q1/50 , C12Y207/0102 , C12Y207/03002 , C12Y207/04003 , C12Y406/01001 , G01N33/573 , G01N33/57484 , G01N2333/01
摘要： Procédé permettant d'inhiber le taux de croissance, la transformation ou métastase de cellules de mammifères, notamment de cellules épithéliales, où l'activité d'un enzyme au moins, participant dans les taux de métabolisme de la purine et de régulation des nucléotides, est élevée. On décrit en particulier un procédé permettant d'inhiber la transformation de cellules de mammifère par un virus oncogène de l'ADN, un facteur de virus oncogène de l'ADN, ou par d'autres facteurs ayant un effet semblable sur les cellules.
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3.发明公开ISOLATION OF FACTORS THAT ASSOCIATE DIRECTLY OR INDIRECTLY WITH NON-CODING RNAS 有权隔离直接与非编码RNA，间接的关联因素的
公开(公告)号：EP2744902A1
公开(公告)日：2014-06-25
申请号：EP12825262.4
申请日：2012-08-20
IPC分类号： C12N15/113 , C12Q1/68 , G01N33/553
CPC分类号： C12Q1/6834 , C12Q1/6809 , C12Q1/6811 , C12Q2523/101 , C12Q2525/207 , C12Q2565/133 , C12Q2522/101 , C12Q2563/131 , C12Q2523/303 , C12Q2563/149
摘要： Methods and assays are provided for isolating factors including polypeptides, ribonucleic acids (RNAs) and polypeptide complexes that are associated with a target nucleic acid sequence. The target nucleic acid sequence may be comprised within chromatin. The methods are suitable for identification and characterisation of factors including non-coding RNAs (ncRNAs) that associate with specified genomic loci.
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4.发明公开ISOLATION OF PROTEIN FACTORS THAT ASSOCIATE DIRECTLY OR INDIRECTLY WITH NUCLEIC ACIDS 有权明示或暗示的关联蛋白因子WITH NUCLEIC的隔震
公开(公告)号：EP2191016A1
公开(公告)日：2010-06-02
申请号：EP08788385.6
申请日：2008-08-20
发明人： KINGSTON, Robert , DEJARDIN, Jerome
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C07K1/14 , C12Q1/6834 , C12Q2563/149 , C12Q2563/131 , C12Q2525/113
摘要： Methods for isolating polypeptides and polypeptide complexes that are associated with a target nucleic acid sequence are provided. The methods comprise the steps of obtaining a sample that comprises a target nucleic acid sequence and one or more polypeptides or proteins associated with that target nucleic acid sequence; contacting the sample with at least one oligonucleotide probe that comprises a sequence that is complimentary to and capable of hybridising with at least a portion of the target nucleic acid sequence, wherein the oligonucleotide probe comprises at least one locked nucleic acid (LNA) nucleotide and wherein the oligonucleotide probe further comprises at least one affinity label (e.g. biotin); allowing the at least one oligonucleotide probe and the target nucleic acid sequence to hybridise with each other so as to form a probe-target hybrid; isolating the probe-target hybrid from the sample by immobilizing the probe-target hybrid through a molecule that binds to the at least one affinity label (e.g. using streptavidin-coated magnetic beads); and eluting the one or more polypeptides that are associated with the target nucleic acid sequence. Probes (e.g. with long spacer) for use in the methods of screening are also provided.
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5.发明授权ISOLATION OF PROTEIN FACTORS THAT ASSOCIATE DIRECTLY OR INDIRECTLY WITH NUCLEIC ACIDS 有权明示或暗示的关联蛋白因子WITH NUCLEIC的隔震
公开(公告)号：EP2191016B1
公开(公告)日：2015-03-04
申请号：EP08788385.6
申请日：2008-08-20
发明人： KINGSTON, Robert , DEJARDIN, Jerome
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6876 , C07K1/14 , C12Q1/6834 , C12Q2563/149 , C12Q2563/131 , C12Q2525/113
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公开(公告)号：EP2744902B1
公开(公告)日：2018-06-06
申请号：EP12825262.4
申请日：2012-08-20
IPC分类号： C12N15/113 , C12Q1/68 , G01N33/553
CPC分类号： C12Q1/6834 , C12Q1/6809 , C12Q1/6811 , C12Q1/6813 , C12Q1/6876 , C12Q2523/101 , C12Q2525/207 , C12Q2565/133 , C12Q2522/101 , C12Q2563/131 , C12Q2523/303 , C12Q2563/149
摘要： Methods and assays are provided for isolating factors including polypeptides, ribonucleic acids (RNAs) and polypeptide complexes that are associated with a target nucleic acid sequence. The target nucleic acid sequence may be comprised within chromatin. The methods are suitable for identification and characterisation of factors including non-coding RNAs (ncRNAs) that associate with specified genomic loci.
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