公开(公告)号：EP1631577A4

公开(公告)日：2008-07-23

申请号：EP03741837

申请日：2003-05-28

Applicant: MAVEN TECHNOLOGIES LLC

Inventor： RASSMAN WILLIAM ,  RALIN DAVID ,  ZHOU FEIMENG ,  HAN SHUBO

IPC: C07H21/04 ,  C12M3/00 ,  C12Q1/68 ,  G01J4/00 ,  G01N21/00 ,  G01N21/17 ,  G01N21/21 ,  G01N21/55 ,  G01N21/75 ,  G01N33/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/557

CPC classification number: G01N21/21 ,  C07H21/04 ,  G01N21/552 ,  G01N33/54373 ,  G01N2021/757

Abstract: A probe-target reaction is made more recognizable by the provision of a mass-enhancing and/or evanescent-field-perturbing amplifier element which reacts uniquely with and binds to the probe-target pair to provide increased mass. Where the probe-target pair is hybridized dsDNA, a suitable mass­ enhancing amplifier is anti-double stranded DNA mouse IgM. In examples with sufficient sequence pairs in the probe-target combination, a sequence-specific minor-groove-binding polyamide can be used that carries biotin which can be amplified by streptavidin in a suitable carrier. In a preferred embodiment, a plurality of probes are immobilized at the sites of a microarray, each probe being specific to a different target. Optics utilizing total internal reflection are described for observing perturbation of the evanescent field.