公开(公告)号：EP1631577A4

公开(公告)日：2008-07-23

申请号：EP03741837

申请日：2003-05-28

Applicant: MAVEN TECHNOLOGIES LLC

Inventor： RASSMAN WILLIAM ,  RALIN DAVID ,  ZHOU FEIMENG ,  HAN SHUBO

IPC: C07H21/04 ,  C12M3/00 ,  C12Q1/68 ,  G01J4/00 ,  G01N21/00 ,  G01N21/17 ,  G01N21/21 ,  G01N21/55 ,  G01N21/75 ,  G01N33/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/557

CPC classification number: G01N21/21 ,  C07H21/04 ,  G01N21/552 ,  G01N33/54373 ,  G01N2021/757

Abstract: A probe-target reaction is made more recognizable by the provision of a mass-enhancing and/or evanescent-field-perturbing amplifier element which reacts uniquely with and binds to the probe-target pair to provide increased mass. Where the probe-target pair is hybridized dsDNA, a suitable mass­ enhancing amplifier is anti-double stranded DNA mouse IgM. In examples with sufficient sequence pairs in the probe-target combination, a sequence-specific minor-groove-binding polyamide can be used that carries biotin which can be amplified by streptavidin in a suitable carrier. In a preferred embodiment, a plurality of probes are immobilized at the sites of a microarray, each probe being specific to a different target. Optics utilizing total internal reflection are described for observing perturbation of the evanescent field.

公开(公告)号：EP1747432A4

公开(公告)日：2010-06-02

申请号：EP05746623

申请日：2005-05-10

Applicant: MAVEN TECHNOLOGIES LLC

Inventor： RASSMAN WILLIAM ,  RALIN DAVID ,  LIEBERMAN ROBERT A ,  KEMPEN LOTHAR U ,  SHAPIRO HERBERT

IPC: G01J4/00 ,  G01N21/01 ,  G01N21/21 ,  G01N21/25 ,  G01N21/55 ,  G01N33/53

CPC classification number: G01N21/211 ,  G01N21/21 ,  G01N21/253 ,  G01N21/552 ,  G01N2021/212 ,  Y10S436/805

公开(公告)号：EP1747433A4

公开(公告)日：2011-04-06

申请号：EP05749961

申请日：2005-05-10

Applicant: MAVEN TECHNOLOGIES LLC

Inventor： RASSMAN WILLIAM ,  RALIN DAVID ,  LIEBERMAN ROBERT A ,  KEMPEN LOTHAR U ,  SHAPIRO HERBERT

IPC: G01N21/25 ,  G01J4/00 ,  G01N21/21 ,  G01N21/55 ,  G01N33/543

CPC classification number: G01N21/253 ,  G01N21/21 ,  G01N21/211 ,  G01N21/552 ,  G01N33/54373 ,  G01N2021/212

公开(公告)号：EP1470401A4

公开(公告)日：2007-08-29

申请号：EP03702096

申请日：2003-01-10

Applicant: MAVEN TECHNOLOGIES LLC

Inventor： LIEBERMAN ROBERT A ,  RASSMAN WILLIAM ,  KEMPEN LOTHAR U ,  RALIN DAVID

IPC: G01N21/05 ,  G01N21/17 ,  G01J4/00 ,  G01N21/21 ,  G01N21/27 ,  G01N21/55

CPC classification number: G01N21/21 ,  G01N21/552 ,  G01N21/553

Abstract: Apparatus for acquiring an image of a specimen comprising a cassette (300, 302a, 302b) having an optical portion (312) holding a specimen array on a TIR surface (314) and being removably matable to a processing portion having a polarized light beam source and a processing polarization sensitive portion to image the spatially distributed charges in polarization of the specimen array. In one form the array optical portion comprises a transparent slide (312) having a bottom surface with first and second gratings located to direct polarized light to the TIR surface (314) and to direct light reflected by that (TIR) surface to an imager, respectively. The apparatus may include a flow cell (316) integral with the optical portion (312) as well as means for selecting the direction and wavelength of the polarized light.