公开(公告)号：EP1395275A2

公开(公告)日：2004-03-10

申请号：EP02729238.2

申请日：2002-05-17

申请人： Merck & Co., Inc.

发明人： BRUCE, James, E. ,  TANG, Xiaoting ,  GARSKY, Victor ,  BRADY, Stephen, F. ,  LI, Yueming ,  CHEN-DODSON, Elizabeth ,  SARDANA, Mohinder ,  SHAFER, Jules, A.

IPC分类号： A61K38/04 ,  A61K38/08

CPC分类号： C12Q1/37 ,  C07K7/06 ,  C07K14/4711 ,  C12P21/06 ,  G01N33/6896 ,  G01N2500/00

摘要： The present invention provides synthetic β-secretase peptide substrates useful in various assays for measuring β-secretase activity. Antibodies that recognize the synthetic substrates and uses of the antibodies in various assays are disclosed. The herein disclosed peptide substrates are hydrolyzed at rates substantially faster than the attendant Swedish mutant APP from which the substrate sequences are derived.

公开(公告)号：EP0858262B1

公开(公告)日：2002-12-04

申请号：EP96936804.2

申请日：1996-10-21

申请人： MERCK & CO., INC.

发明人： LUMMA, William, C. ,  TUCKER, Thomas, J. ,  WITHERUP, Keith, M. ,  BRADY, Stephen, F. ,  WHITTER, Willie, L. ,  VACCA, Joseph, P. ,  COBURN, Craig ,  SHAFER, Jules, A.

IPC分类号： A01N43/34 ,  A01N43/64 ,  A01N43/82 ,  A61K31/165 ,  C07D207/08

CPC分类号： C07K5/06191 ,  A61K38/00 ,  C07D207/16 ,  C07K5/0222 ,  C07K5/06078 ,  C07K5/06139

公开(公告)号：EP1395275B1

公开(公告)日：2008-11-12

申请号：EP02729238.2

申请日：2002-05-17

申请人： Merck & Co., Inc.

发明人： BRUCE, James, E. ,  TANG, Xiaoting ,  GARSKY, Victor ,  BRADY, Stephen, F. ,  LI, Yueming ,  CHEN-DODSON, Elizabeth ,  SARDANA, Mohinder ,  SHAFER, Jules, A.

IPC分类号： A61K38/04 ,  A61K38/08 ,  C07K14/47 ,  C07K16/18 ,  A61K39/395 ,  C12Q1/37 ,  G01N33/573 ,  G01N33/577

CPC分类号： C12Q1/37 ,  C07K7/06 ,  C07K14/4711 ,  C12P21/06 ,  G01N33/6896 ,  G01N2500/00

公开(公告)号：EP0833900A1

公开(公告)日：1998-04-08

申请号：EP96916677.0

申请日：1996-05-28

申请人： MERCK & CO., INC.

发明人： REGISTER, R., Bruce ,  SHAFER, Jules, A.

IPC分类号： C12N9 ,  C12N15

CPC分类号： C12N9/503 ,  C12N15/102

摘要： A method for introducing a mutation into a desired site in Herpes Simplex Virus Type 1 uses a set of starting vectors, where each starting vector has a fragment of the substantially complete HSV-1 genome and also DNA which overlaps with a sequential genomic fragment contained in other starting vectors, so that upon co-transfection of a host cell, replication of viral DNA, and recombination, a mutated replicable virus is formed. The starting vector containing a gene which is to be mutated is replaced by replacement vectors. The replacement vectors contain a copy of the mutated gene and overlapping DNA, and genomic DNA which was present in the starting vector. A host cell is transformed with the replacement vectors and the remaining starting vectors under conditions allowing replication of viral DNA and recombination to form a replicating mutated virus. In preferred embodiments, the protease gene is mutated.

公开(公告)号：EP0858262A1

公开(公告)日：1998-08-19

申请号：EP96936804.0

申请日：1996-10-21

申请人： MERCK & CO., INC.

发明人： LUMMA, William, C. ,  TUCKER, Thomas, J. ,  WITHERUP, Keith, M. ,  BRADY, Stephen, F. ,  WHITTER, Willie, L. ,  VACCA, Joseph, P. ,  COBURN, Craig ,  SHAFER, Jules, A.

IPC分类号： A01N43 ,  C07D207 ,  C07K5 ,  A61K38

CPC分类号： C07K5/06191 ,  A61K38/00 ,  C07D207/16 ,  C07K5/0222 ,  C07K5/06078 ,  C07K5/06139

摘要： A compound which inhibits human thrombin and where has the structure (I) such as (II).

摘要翻译： 抑制人凝血酶的化合物，其中具有结构（I）如（II）的化合物。