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    SINGLE TUBE BEAD-BASED DNA CO-BARCODING FOR ACCURATE AND COST-EFFECTIVE SEQUENCING, HAPLOTYPING, AND ASSEMBLY
    2.
    发明公开
    SINGLE TUBE BEAD-BASED DNA CO-BARCODING FOR ACCURATE AND COST-EFFECTIVE SEQUENCING, HAPLOTYPING, AND ASSEMBLY 审中-公开

    公开(公告)号：EP4245861A2

    公开(公告)日：2023-09-20

    申请号：EP23168214.7

    申请日：2019-05-07

    申请人： MGI Tech Co., Ltd. ,  BGI Shenzhen

    发明人： DRMANAC, Radoje T. ,  PETERS, Brock A. ,  WANG, Ou

    IPC分类号： C12Q1/6806

    摘要： Methods and compositions for preparing a nucleic acid sequencing library are described including (a)transposing an insertion sequence into first fragments of the target nucleic acid, wherein the insertion sequence comprises a hybridization sequence, and wherein the transposing produces nicks in the first fragments; (b) combining in a single mixture (i) the first fragments of the target nucleic acid from (a), (ii) a splint oligonucleotide, and (iii) a population of beads, wherein each bead comprises capture oligonucleotides immobilized thereon, and (c) ligating capture oligonucleotides of individual beads to inserted hybridization sequences of individual first fragments.