公开(公告)号：EP1283265A4

公开(公告)日：2004-10-20

申请号：EP01930179

申请日：2001-05-16

申请人： MITSUBISHI PHARMA CORP

发明人： MIURA MASAMI ,  HIROSE MASAAKI ,  MIWA TAEKO ,  IRIE HIROYUKI ,  KUWAE SHINOBU ,  MIYANO KENMI ,  OTANI WATARU ,  OHI HIDEYUKI

IPC分类号： C12N1/19 ,  C12N9/12 ,  C12P21/00 ,  C12N15/52 ,  C07K14/46 ,  C07K16/18 ,  C12P21/02 ,  C12R1/84

CPC分类号： C12N9/1205 ,  C12P21/005

摘要： It is intended to find out a gene participating in the addition of mannose phosphate to a sugar chain of a glycoprotein originating in a yeast belonging to the genus Pichia and provide a means of controlling the same. It is also intended to provide a process for producing a protein with reduction of an acidic sugar chain by using the thus controlled yeast strain belonging to the genus Pichia. Namely, a protein participating in the addition of mannose phosphate to a sugar chain of a glycoprotein; a gene encoding this protein; a variant of this gene; a vector carrying the variant gene; a yeast belonging to the genus Pichia having been transformed by this vector; a process for producing a protein with reduction of an acidic sugar chain by using the transformed yeast strain; and a glycoprotein thus produced.

摘要翻译： 本发明的意图，找出一个基因参与加成磷酸甘露糖的一种糖蛋白的起源的糖链在属于毕赤酵母属的酵母，并且提供控制该的手段。 因此，本发明意图用于生产蛋白质与还原通过使用属于毕赤酵母属如此控制的酵母菌株的酸性糖链的方法。 即，本发明包括参与除了磷酸甘露糖的糖蛋白的糖链的蛋白质; 的基因编码该蛋白质; 此基因的突变体; 携带突变型基因的载体; 属于毕赤酵母属的酵母菌株已经被转化的由该向量; 用于生产具有减少通过使用转化的酵母菌株的酸性糖链的蛋白质的方法; 和糖蛋白如此产生。