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    Process for purifying tryptophan
    1.
    发明公开
    Process for purifying tryptophan 失效
    一种纯化色氨酸过程。

    公开(公告)号：EP0200944A2

    公开(公告)日：1986-11-12

    申请号：EP86104915.3

    申请日：1986-04-10

    申请人： MITSUI TOATSU CHEMICALS, Inc.

    发明人： Miyahara, Shoichiro ,  Matsumoto, Toshio ,  Miyahara, Tooru ,  Nitta, Kazunari

    IPC分类号： C12P13/22

    CPC分类号： C07D209/20 ,  C12P13/227

    摘要： A process for purifying tryptophan so as to contain almost no unreacted indole and a process for recovering unreacted indole with a very high percentage are provided, the purififaction process comprising passing a reaction fluid of tryptophan prepared from indole as raw material by means of an enzyme function, through a porous type, cation exchange resin layer, to cause the ion exchange part of the resin to adsorb tryptophan, while to cause the porous part of the resin to adsorb indole, followed by eluting only the tryptophan with an alkali or acid aqueous solution, and the recovery process comprising subjecting tryptophan to repeated treatments of adsorption onto and elution from the ion exchange part of the resin and regeneration followed by eluting indole adsorbed onto the porous part of the resin with a water-containing organic solvent miscible with water and isolating indole from the resulting elute.

    Process for preparing L-tryptophan
    2.
    发明公开
    Process for preparing L-tryptophan 失效
    制备L-酪氨酸的方法和制备稳定的水性酵素溶液的方法

    公开(公告)号：EP0299715A3

    公开(公告)日：1990-07-04

    申请号：EP88306349.7

    申请日：1988-07-12

    申请人： MITSUI TOATSU CHEMICALS, Inc.

    发明人： Miyahara, Shoichiro ,  Yanaka, Makoto ,  Matsumoto, Toshio ,  Miyahara, Tooru ,  Sakaguchi, Akio ,  Nitta, Kazunari

    IPC分类号： C12P13/22 ,  C12N9/88 ,  C12N9/96

    CPC分类号： C12N9/88 ,  C12N9/00 ,  C12N9/96 ,  C12P13/227

    摘要： A method of preparing L-tryptophan by reacting indole and serine and using an aqueous solution of enzymes which are extracted from cells. After the reaction, the pH value of the reaction mass is adjusted to above 10 by ammonia. The L-tryptophan can be dissolved in the solution in a high concentration, so purification and separation are facilitated and the loss of L-tryptophan minimalized. The preparation of a stable aqueous solution of enzymes is also provided wherein an extracted enzyme solution is heated above 40°C.

    摘要翻译： 一种通过吲哚和丝氨酸反应并使用从细胞中提取的酶水溶液制备L-色氨酸的方法。 反应后，用氨将反应物质的pH值调至10以上。 L-色氨酸可以以高浓度溶解在溶液中，因此促进了纯化和分离，并减少了L-色氨酸的损失。 还提供了稳定的酶水溶液的制备，其中将提取的酶溶液加热至40℃以上。