公开(公告)号：EP1702072A4

公开(公告)日：2007-03-21

申请号：EP03767310

申请日：2003-12-23

申请人： MURDOCH CHILDRENS RES INST

发明人： CHOO ANDY ,  SAFFERY RICHARD ERIC ,  WONG LEE HWA

IPC分类号： C12N15/85 ,  A61K48/00 ,  C12N15/12 ,  C12N15/79 ,  C12N15/82 ,  C12N15/90

CPC分类号： A61K48/005 ,  C12N15/82 ,  C12N15/85 ,  C12N15/8509 ,  C12N2800/30 ,  C12N2830/002 ,  C12N2830/003 ,  C12N2830/15 ,  C12N2830/46

摘要： The present invention provides a target region within a mammalian, avian, plant or other eukaryotic chromosome or an artificial or engineered chromosomal construct which is capable of carrying and expressing a heterologous gene or other genetic molecule of interest. The gene or genetic molecule of interest is expressed in a region of the chromosome which corresponds to or which immediately adjoins or is proximal to a centromeric or neocentromeric region or a functional derivative thereof or a latent, synthetic or hybrid form thereof. A method for facilitating genetic therapy or genetic modification or other applications are also provided including protein production for proteomic therapy in a mammal, avian species or plant or other higher eukaryotes.

公开(公告)号：EP1354055A4

公开(公告)日：2004-07-07

申请号：EP01271443

申请日：2001-12-20

申请人： MURDOCH CHILDRENS RES INST

发明人： CHOO KONG-HONG ANDY ,  WONG LEE HWA ,  SAFFERY RICHARD ERIC

IPC分类号： G01N33/53 ,  A61K31/7088 ,  A61K48/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/68 ,  C12N15/85 ,  C12Q1/68 ,  G01N33/566 ,  C12N15/64 ,  C12N15/79

CPC分类号： C12N15/68 ,  A61K48/00 ,  C12N15/85 ,  C12N2800/204 ,  C12N2800/30

摘要： The present invention is directed generally to a defined or isolated nucleic acid molecule encompassing a neocentreomere or a functional derivative thereof or a latent, synthetic or hybrid form thereof and its use inter alia in developing a range of eukaryotic mini-chromosomes and artificial chromosomes including mammalian (e.g. human) and non-mammalian mini-chromosomes and artificial chromosomes. The present invention further provides a telomere-associated chromosome truncation (TACT) approach to develop mini-chromosomes, but is not limited to this approach. It is directed to any truncation approach that produces a neocentromere-containing mini-chromosome, or any transfection approach using cloned or pre-fabricated DNA that provides neocentromere function in the construction of artificial chromosome. Such mini-chromosomes and artificial chromosomes are useful in a range of genetic therapies.