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    Gene knockout mesophilic and thermophilic organisms, and methods of use thereof
    2.
    发明公开
    Gene knockout mesophilic and thermophilic organisms, and methods of use thereof 审中-公开
    Mesophile und thermophile Knockout-Organismen und Verfahren zu deren Verwendung

    公开(公告)号：EP2511285A2

    公开(公告)日：2012-10-17

    申请号：EP12164671.5

    申请日：2008-05-09

    申请人： Mascoma Corporation

    发明人： Hogsett, David A. ,  Rajgarhia, Vineet B.

    IPC分类号： C07H21/02 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C12P7/10 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/1217 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12N15/74 ,  C12P7/065 ,  C12P2203/00 ,  C12Y101/01002 ,  C12Y101/01027 ,  C12Y101/02003 ,  C12Y203/01008 ,  C12Y207/01017 ,  C12Y207/02001 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17

    摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce ethanol as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non- native gene encodes a first non-native enzyme involved in the metabolic production of ethanol.; Another aspect of the invention relates to a process for converting lignocellulosic biomass to ethanol, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

    摘要翻译： 本发明的一个方面涉及经遗传修饰的嗜热或嗜温微生物，其中第一天然基因部分，基本上或完全缺失，沉默，失活或下调，其中第一天然基因编码参与 有机酸或其盐的代谢产生，从而增加所述嗜热或嗜温微生物产生乙醇作为发酵产物的天然能力。 在某些实施方案中，上述微生物还包含第一非天然基因，其第一非天然基因编码参与乙醇代谢生产的第一非天然酶。 本发明的另一方面涉及将木质纤维素生物质转化为乙醇的方法，包括使木质纤维素生物质与经遗传修饰的嗜热或嗜温微生物接触。