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公开(公告)号：EP2813582B1
公开(公告)日：2017-04-12
申请号：EP14176605.5
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie (EMBL)
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K47/549 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
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2.发明公开
公开(公告)号：EP2348134A1
公开(公告)日：2011-07-27
申请号：EP10180025.8
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie (EMBL)
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K47/549 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
摘要翻译： 双链RNA（dsRNA）通过称为RNA干扰（RNAi）的过程在许多生物体中诱导序列特异性转录后基因沉默。 使用果蝇体外系统，我们证明19-23短小RNA片段是RNAi的序列特异性介质。 短干扰RNA（siRNA）通过来自长dsRNA的RNA酶III类加工反应产生。 与突出的3'末端的化学合成的siRNA双链体介导裂解物中有效的靶RNA切割，并且切割位点位于由引导siRNA跨越的区域的中心附近。 此外，我们提供证据表明dsRNA加工的方向决定了所产生的siRNP复合物是否可以切割有义或反义的靶RNA。
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3.发明公开
公开(公告)号：EP2348133A1
公开(公告)日：2011-07-27
申请号：EP10179952.6
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie (EMBL)
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
摘要翻译： 双链RNA（dsRNA）通过称为RNA干扰（RNAi）的过程在许多生物体中诱导序列特异性转录后基因沉默。 使用果蝇体外系统，我们证明19-23短小RNA片段是RNAi的序列特异性介质。 短干扰RNA（siRNA）通过来自长dsRNA的RNA酶III类加工反应产生。 与突出的3'末端的化学合成的siRNA双链体介导裂解物中有效的靶RNA切割，并且切割位点位于由引导siRNA跨越的区域的中心附近。 此外，我们提供证据表明dsRNA加工的方向决定了所产生的siRNP复合物是否可以切割有义或反义的靶RNA。
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4.发明公开Inducible small interfering RNA (siRNA) expression constructs for targeted gene silencing 有权INDUZIERBARE SIRNA EXPRESSIONSKONSTRUKTE ZUR GEZIELTEN GENABSCHALTUNG
公开(公告)号：EP2314687A1
公开(公告)日：2011-04-27
申请号：EP10179492.3
申请日：2004-01-15
IPC分类号： C12N15/11
CPC分类号： C12N15/111 , A01K2217/05 , A01K2217/075 , A61K38/00 , A61K48/0058 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： The invention relates to vectors for the inducible expression of RNA molecules in eukaryotic, particularly mammalian cells and transgenic animals.
摘要翻译： 本发明涉及用于在真核细胞，特别是哺乳动物细胞和转基因动物中可诱导表达RNA分子的载体。
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公开(公告)号：EP2348134B1
公开(公告)日：2014-04-16
申请号：EP10180025.8
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie (EMBL)
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K47/549 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
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公开(公告)号：EP2351852B1
公开(公告)日：2013-10-02
申请号：EP10179947.6
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie (EMBL)
IPC分类号： C12N15/113 , A61K47/48
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K47/549 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
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7.发明公开
公开(公告)号：EP2351852A1
公开(公告)日：2011-08-03
申请号：EP10179947.6
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , Europäisches Laboratorium für Molekularbiologie (EMBL)
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K47/549 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
摘要翻译： 双链RNA（dsRNA）通过称为RNA干扰（RNAi）的过程在许多生物体中诱导序列特异性转录后基因沉默。 使用果蝇体外系统，我们证明19-23短小RNA片段是RNAi的序列特异性介质。 短干扰RNA（siRNA）通过来自长dsRNA的RNA酶III类加工反应产生。 与突出的3'末端的化学合成的siRNA双链体介导裂解物中有效的靶RNA切割，并且切割位点位于由引导siRNA跨越的区域的中心附近。 此外，我们提供证据表明dsRNA加工的方向决定了所产生的siRNP复合物是否可以切割有义或反义的靶RNA。
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8.发明授权INDUCIBLE SMALL INTERFERING RNA (SIRNA) EXPRESSION CONSTRUCTS FOR TARGETED GENE SILENCING 有权可诱导的小干扰RNA（SIRNA）表达构建靶向基因沉默
公开(公告)号：EP2314687B1
公开(公告)日：2017-12-27
申请号：EP10179492.3
申请日：2004-01-15
IPC分类号： C12N15/11
CPC分类号： C12N15/111 , A01K2217/05 , A01K2217/075 , A61K38/00 , A61K48/0058 , C12N2310/111 , C12N2310/14 , C12N2310/53 , C12N2330/30
摘要： The invention relates to vectors for the inducible expression of RNA molecules in eukaryotic, particularly mammalian cells and transgenic animals.
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公开(公告)号：EP1873259B1
公开(公告)日：2012-01-25
申请号：EP07014533.9
申请日：2001-11-29
申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. , EUROPÄISCHES LABORATORIUM FÜR MOLEKULARBIOLOGIE (EMBL)
IPC分类号： C12N15/113
CPC分类号： C12N15/113 , A01K2217/075 , A61K9/0019 , A61K38/00 , A61K48/00 , C12N15/1079 , C12N15/111 , C12N2310/14 , C12N2310/321 , C12N2310/53 , C12N2330/30 , C12N2310/3521
摘要： Double-stranded RNA (dsRNA) induces sequence-specific post-transcriptional gene silencing in many organisms by a process known as RNA interference (RNAi). Using a Drosophila in vitro system, we demonstrate that 19-23 nt short RNA fragments are the sequence-specific mediators of RNAi. The short interfering RNAs (siRNAs) are generated by an RNase III-like processing reaction from long dsRNA. Chemically synthesized siRNA duplexes with overhanging 3' ends mediate efficient target RNA cleavage in the lysate, and the cleavage site is located near the center of the region spanned by the guiding siRNA. Furthermore, we provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the produced siRNP complex.
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公开(公告)号：EP2314690A1
公开(公告)日：2011-04-27
申请号：EP10178802.4
申请日：2003-07-10
发明人： Tuschl, Thomas , Martinez, Javier , Patkaniowska, Agnieszka , Urlaub, Henning , Lührmann, Reinhard
CPC分类号： C12N15/113 , A61K47/549 , A61K47/557 , C07K14/4705 , C07K19/00 , C12N15/111 , C12N2310/14 , C12N2310/351 , C12N2310/3513 , C12N2320/30 , C12N2320/51 , C12N2330/30
摘要： The present invention relates to sequence and structural features of single-stranded (ss) RNA molecules required to mediate target-specific nucleic acid modifications by RNA-interference (RNAi), such as target mRNA degradation and/or DNA methylation.
摘要翻译： 本发明涉及通过RNA干扰（RNAi）介导靶特异性核酸修饰（例如靶mRNA降解和/或DNA甲基化）所需的单链（ss）RNA分子的序列和结构特征。
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