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    Molecular coding for analysis of composition of macromolecules and molecular complexes
    1.
    发明公开
    Molecular coding for analysis of composition of macromolecules and molecular complexes 审中-公开
    Molekularcodierungfürdie Analyze eine Zusammensetzung ausMakromolekülenund Molekularkomplexe

    公开(公告)号：EP2749653A1

    公开(公告)日：2014-07-02

    申请号：EP12199781.1

    申请日：2012-12-28

    申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

    发明人： Borodina, Tatiana, Dr. ,  Soldatov, Aleksey, Dr. ,  Lehrach, Hans, Prof.

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6869 ,  G01N2458/10 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2563/159 ,  C12Q2563/179

    摘要： The present invention relates to a method for identification of fragments originating from individual macromolecules (MM) or molecular complexes (MC) in a mixture of fragments of different MM or MC using labeling of MM or MC with oligonucleotide markers comprising the following steps:
    a) labeling of MM or MC with oligonucleotide markers wherein each particular MM or MC is labeled with identical oligonucleotide markers and preferentially the different MM or MC are labeled with different oligonucleotide markers and wherein the number of identical oligonucleotide markers is sufficient that after subsequent fragmentation or dissociation of fragments of the MM or the MC each fragment is preferentially labeled with at least one of the oligonucleotide marker;
    b) fragmentation or dissociation of MM or MC, wherein step a) and b) are optionally done in parallel;
    c) mixing labeled fragments of different MM or MC together;
    d) analyzing of fragments and determining the nucleotide sequence of the at least one oligonucleotide marker associated with each fragment;
    e) identification of fragments originating from individual MM or MC of fragments based on the fact that fragments associated with different oligonucleotide markers were part of different MM or MC before said fragmentation.

    摘要翻译： 本发明涉及用MM或MC片段的混合物鉴定不同MM或MC片段的单个大分子（MM）或分子复合物（MC）的片段的方法，所述方法用MM或MC标记寡核苷酸标记物，其包括以下步骤：a） 用寡核苷酸标记物标记MM或MC，其中每个特定的MM或MC用相同的寡核苷酸标记物标记，优选不同的MM或MC用不同的寡核苷酸标记物标记，并且其中相同寡核苷酸标记的数目足以使得在随后的分裂或解离之后 MM或MC每个片段的片段优先用寡核苷酸标记中的至少一个标记; b）MM或MC的分解或解离，其中步骤a）和b）任选并行进行; c）将不同MM或MC的标记片段混合在一起; d）分析片段并测定与每个片段相关的至少一个寡核苷酸标记的核苷酸序列; e）基于与不同寡核苷酸标记相关的片段在所述片段化之前是不同MM或MC的一部分的事实，鉴定源自单个MM或MC片段的片段。