公开(公告)号：EP2980223A3

公开(公告)日：2016-04-20

申请号：EP15178875.9

申请日：2015-07-29

申请人： FEI Company

发明人： Utlaut, Mark ,  Parker, N. William

IPC分类号： C12Q1/68 ,  H01J37/20 ,  G01N33/58 ,  C12N15/10

CPC分类号： C12N15/1065 ,  B01J2219/00605 ,  B01J2219/00623 ,  B01J2219/00722 ,  C12Q1/6806 ,  H01J37/20 ,  H01J2237/2004 ,  H01J2237/2802

摘要： A functionalized specimen support for use in charged particle microscopy is provided that includes a specimen support surface configured to support specimens (e.g. DNA strands 1051) during an interrogation of the specimens with a charged particle microscope, the specimen support surface having functionalized sites 1031, each functionalized site configured to maintain position of a portion of one of the specimens at the functionalized site by way of attachment, attraction, or a combination thereof.

公开(公告)号：EP2544990A1

公开(公告)日：2013-01-16

申请号：EP11753874.4

申请日：2011-03-07

申请人： Brookhaven Science Associates

发明人： GANG, Oleg ,  VAN DER LELIE, Daniel

IPC分类号： B82Y40/00

CPC分类号： C12Q1/6837 ,  B01J2219/00612 ,  B01J2219/00623 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00729 ,  B82B3/0014 ,  B82B3/0047 ,  B82Y30/00 ,  B82Y40/00

摘要： The present invention is directed to nanoscale fabrication of nano-materials with application in electronics, energy conversion, bio-sensing and others. Specifically, the invention is directed to arbitrary, that is periodic and non-periodic, assembly of nano-objects on I D and 2D arrays. The present invention utilizes self-organization properties of nanoscale bio-encoded building blocks, programmability of biomolecular interactions, and simple processing techniques for providing arbitrary by-design fabrication capability. Specifically, the present invention utilizes double stranded DNA attached to a surface and intercalating PNA-DNA hybrids attached to nano-objects to bind the nano-objects to the dsDNA in a site specific manner. The present invention allows for an integration of a large number of nano-components in unified well-defined systems. Accordingly, the present invention is applicable for fabrication of I D and 2D structures of various by-design placements of nano-objects of multiple types, including metal, semiconducting and organic nano-objects.

公开(公告)号：EP2204650A1

公开(公告)日：2010-07-07

申请号：EP08022585.7

申请日：2008-12-31

申请人： DWI an der RWTH Aachen e.V.

发明人： Groll, Jürgen, Dr. ,  Möller, Martin ,  Eberhardt, Matthias, Dr.

IPC分类号： G01N33/543 ,  B01J19/00

CPC分类号： G01N33/54393 ,  B01J2219/00509 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/00623 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00702 ,  G01N33/54373

摘要： The present invention relates to a label-free biosensor system, a method for manufacturing said label-free biosensor system, its use for detecting biochemical reactions and/or bindings, enzymatic reactions, nucleic acid hybridizations, protein-protein interactions and protein-ligand interactions, as well as an assay method for detecting and/or quantifying an analyte of interest in a biological sample which comprises detecting the Recognition Induced Birefringence (RIB) generated in the presence as opposed to the absence of said analyte by bringing said sample into contact with said label-free biosensor system.

摘要翻译： 本发明涉及无标记生物传感器系统，制造所述无标记生物传感器系统的方法，其用于检测生化反应和/或结合的用途，酶反应，核酸杂交，蛋白质 - 蛋白质相互作用和蛋白质 - 配体相互作用 以及用于检测和/或定量生物样品中感兴趣分析物的测定方法，其包括检测在存在时产生的识别诱导双折射（RIB），而不是通过使所述样品与所述分析物接触而不存在所述分析物 所述无标签的生物传感器系统。

公开(公告)号：EP3234103A1

公开(公告)日：2017-10-25

申请号：EP15869471.1

申请日：2015-12-15

申请人： Novellusdx Ltd.

发明人： SHEINMAN, Yehoshua ,  SHARIVKIN, Revital ,  BELENKOVICH, Merav

IPC分类号： C12M1/34 ,  C40B30/04 ,  C12Q1/68

CPC分类号： C12N15/1082 ,  B01J19/0046 ,  B01J2219/00376 ,  B01J2219/00387 ,  B01J2219/00497 ,  B01J2219/00596 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00623 ,  B01J2219/00659 ,  B01J2219/00743 ,  C12N15/87

摘要： The present disclosure provides systems, kits, devices and methods for indirect transfer of multiple sets of nucleic-acid and other molecules to cells as exemplified by indirect transfection of sets of nucleic-acid molecules to viable cells.

摘要翻译： 本公开提供了用于将多组核酸分子和其他分子间接转移至细胞的系统，试剂盒，装置和方法，如通过将核酸分子组间接转染至活细胞所举例说明的。

公开(公告)号：EP2833998A1

公开(公告)日：2015-02-11

申请号：EP13713908.5

申请日：2013-04-03

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： SOLDATOV, Aleksey ,  BORODINA, Tatiana ,  LEHRACH, Hans

IPC分类号： B01J19/00 ,  C12Q1/68

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00623 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/125 ,  C12Q2565/513 ,  C12Q2565/514 ,  C12Q2565/518

摘要： The present invention describes a method for identification of areas of a sample from which nucleic acid molecules originate using labeling of said nucleic acid molecules by two-dimensionally distributed oligonucleotide markers. Further analysis of hybrids between the nucleic acid molecules and the oligonucleotide markers allow identification of the original position of the labelled nucleic acid molecules in the sample.

公开(公告)号：EP2647426A1

公开(公告)日：2013-10-09

申请号：EP12163055.2

申请日：2012-04-03

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： Soldatov, Aleksey, Dr. ,  Borodina, Tatiana, Dr. ,  Lehrach, Hans, Prof.

IPC分类号： B01J19/00 ,  C12Q1/68

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00623 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/125 ,  C12Q2565/513 ,  C12Q2565/514 ,  C12Q2565/518

摘要： The present invention provides methods for preserving information about original spatial distribution of nucleic acid molecules transferred from a sample to another surface or into solution.

摘要翻译： 本发明提供了用于保存有关从样品转移到另一个表面上或溶液的核酸分子的原始空间分布的信息的方法。

公开(公告)号：EP2980223A2

公开(公告)日：2016-02-03

申请号：EP15178875.9

申请日：2015-07-29

申请人： FEI Company

发明人： Utlaut, Mark ,  Parker, N. William

IPC分类号： C12Q1/68 ,  H01J37/20 ,  G01N33/58

CPC分类号： C12N15/1065 ,  B01J2219/00605 ,  B01J2219/00623 ,  B01J2219/00722 ,  C12Q1/6806 ,  H01J37/20 ,  H01J2237/2004 ,  H01J2237/2802

摘要： A functionalized specimen support for use in charged particle microscopy is provided that includes a specimen support surface configured to support specimens (e.g. DNA strands 1051) during an interrogation of the specimens with a charged particle microscope, the specimen support surface having functionalized sites 1031, each functionalized site configured to maintain position of a portion of one of the specimens at the functionalized site by way of attachment, attraction, or a combination thereof.

摘要翻译： 提供了一种用于在带电粒子显微镜使用的官能化的样品支持并包括被配置为与带电粒子显微镜试样的询问期间，支持样品（例如DNA链1051）的样品支承表面，试样支撑表面具有官能化位点1031，每个 官能化位点被配置成维持所述样本中的一个的一部分的位置处通过附着，吸附，或它们的组合的方式官能化的位点。

公开(公告)号：EP2833997A1

公开(公告)日：2015-02-11

申请号：EP13713907.7

申请日：2013-04-03

申请人： Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V.

发明人： SOLDATOV, Aleksey ,  BORODINA, Tatiana ,  LEHRACH, Hans

IPC分类号： B01J19/00 ,  C12Q1/68

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00533 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00608 ,  B01J2219/00623 ,  B01J2219/00659 ,  B01J2219/00722 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q2565/125 ,  C12Q2565/513 ,  C12Q2565/514 ,  C12Q2565/518

摘要： The present invention provides methods for replication of nucleic acid molecules distributed on a surface or within a layer by transferring them to a target surface covered with oligonucleotides, and fixation of transferred molecules by hybridization to complementary sequences.

摘要翻译： 本发明提供了保存关于从样品转移到另一表面或溶液中的核酸分子的原始空间分布的信息的方法。

公开(公告)号：EP1982184B1

公开(公告)日：2014-03-26

申请号：EP07708571.0

申请日：2007-01-23

申请人： Kim, So Youn

发明人： Kim, So Youn

IPC分类号： G01N33/53 ,  G01N33/543

CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01J2219/0061 ,  B01J2219/00623 ,  B01J2219/00725 ,  B01J2219/00756

公开(公告)号：EP1982184A1

公开(公告)日：2008-10-22

申请号：EP07708571.0

申请日：2007-01-23

申请人： Kim, So Youn

发明人： Kim, So Youn

IPC分类号： G01N33/53

CPC分类号： G01N33/54393 ,  B01J19/0046 ,  B01J2219/0061 ,  B01J2219/00623 ,  B01J2219/00725 ,  B01J2219/00756

摘要： The present invention relates to a method for screening a sol composition for sol-gel biochips, which is used to immobilize a probe on a surface-untreated substrate, also relates to a sol composition screened by said method and a sol-gel biochip comprising said sol composition immobilized thereon. The sol composition screened by the disclosed method can be mixed with a probe, and the sol mixture can be integrated on 96-well plates, which are widely used in existing bioassays, without surface treatment. Also, the biochip can provide a sensitive and specific good analysis results because this immobilization methods of probe maintain the nature of probes without modification.